Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109978 |
Resumo: | Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, |
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Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility.Cell-wall acylationLignocellulosic feedstockSugarcaneBiomassBiofuelsBackground: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane,Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE.SOUZA, W. R. dePACHECO, T. F.DUARTE, K. E.SAMPAIO, B. L.OLIVEIRA, P. A. deMARTINS, P. M.SANTIAGO, T. R.FORMIGHIERI, E. F.VINECKY, F.RIBEIRO, A. P.DIAS, B. B. A.KOBAYASHI, A. K.MITCHELL, R. A. C.RODRIGUES, D. de S.MOLINARI, H. B. C.2019-06-22T00:35:25Z2019-06-22T00:35:25Z2019-06-2120192019-11-18T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109978enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-06-22T00:35:32Zoai:www.alice.cnptia.embrapa.br:doc/1109978Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-06-22T00:35:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-06-22T00:35:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
title |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
spellingShingle |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. SOUZA, W. R. de Cell-wall acylation Lignocellulosic feedstock Sugarcane Biomass Biofuels |
title_short |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
title_full |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
title_fullStr |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
title_full_unstemmed |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
title_sort |
Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. |
author |
SOUZA, W. R. de |
author_facet |
SOUZA, W. R. de PACHECO, T. F. DUARTE, K. E. SAMPAIO, B. L. OLIVEIRA, P. A. de MARTINS, P. M. SANTIAGO, T. R. FORMIGHIERI, E. F. VINECKY, F. RIBEIRO, A. P. DIAS, B. B. A. KOBAYASHI, A. K. MITCHELL, R. A. C. RODRIGUES, D. de S. MOLINARI, H. B. C. |
author_role |
author |
author2 |
PACHECO, T. F. DUARTE, K. E. SAMPAIO, B. L. OLIVEIRA, P. A. de MARTINS, P. M. SANTIAGO, T. R. FORMIGHIERI, E. F. VINECKY, F. RIBEIRO, A. P. DIAS, B. B. A. KOBAYASHI, A. K. MITCHELL, R. A. C. RODRIGUES, D. de S. MOLINARI, H. B. C. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Wagner Rodrigo de Souza, Bolsista da Embrapa Agroenergia; THALYTA FRAGA PACHECO, CNPAE; Karoline Estefani Duarte, Bolsista da Embrapa Agroenergia; Bruno Leite Sampaio, Bolsista da Embrapa Agroenergia; PATRICIA ABRAO DE OLIVEIRA MOLINARI, CNPAE; Polyana Kelly Martins, Bolsista da Embrapa Agroenergia; Thaís Ribeiro Santiago, Bolsista da Embrapa Agroenergia; EDUARDO FERNANDES FORMIGHIERI, CNPAE; Felipe Vinecky; Ana Paula Ribeiro; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; ADILSON KENJI KOBAYASHI, CNPAE; Rowan Andrew Craig Mitchell; DASCIANA DE SOUSA RODRIGUES, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
dc.contributor.author.fl_str_mv |
SOUZA, W. R. de PACHECO, T. F. DUARTE, K. E. SAMPAIO, B. L. OLIVEIRA, P. A. de MARTINS, P. M. SANTIAGO, T. R. FORMIGHIERI, E. F. VINECKY, F. RIBEIRO, A. P. DIAS, B. B. A. KOBAYASHI, A. K. MITCHELL, R. A. C. RODRIGUES, D. de S. MOLINARI, H. B. C. |
dc.subject.por.fl_str_mv |
Cell-wall acylation Lignocellulosic feedstock Sugarcane Biomass Biofuels |
topic |
Cell-wall acylation Lignocellulosic feedstock Sugarcane Biomass Biofuels |
description |
Background: Sugarcane (Saccharum spp.) covers vast areas of land (around 25 million ha worldwide), and its processing is already linked into infrastructure for producing bioethanol in many countries. This makes it an ideal candidate for improving composition of its residues (mostly cell walls), making them more suitable for cellulosic ethanol production. In this paper, we report an approach to improving saccharification of sugarcane straw by RNAi silencing of the recently discovered BAHD01 gene responsible for feruloylation of grass cell walls. Results: We identified six BAHD genes in the sugarcane genome (SacBAHDs) and generated five lines with substantially decreased SacBAHD01 expression. To find optimal conditions for determining saccharification of sugarcane straw, we tried multiple combinations of solvent and temperature pretreatment conditions, devising a predictive model for finding their effects on glucose release. Under optimal conditions, demonstrated by Organosolv pretreatment using 30% ethanol for 240 min, transgenic lines showed increases in saccharification efficiency of up to 24%. The three lines with improved saccharification efficiency had lower cell-wall ferulate content but unchanged monosaccharide and lignin compositions. Conclusions: The silencing of SacBAHD01 gene and subsequent decrease of cell-wall ferulate contents indicate a promising novel biotechnological approach for improving the suitability of sugarcane residues for cellulosic ethanol production. In addition, the Organosolv pretreatment of the genetically modified biomass and the optimal conditions for the enzymatic hydrolysis presented here might be incorporated in the sugarcane industry for bioethanol production. Keywords: Sugarcane, |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-22T00:35:25Z 2019-06-22T00:35:25Z 2019-06-21 2019 2019-11-18T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109978 |
identifier_str_mv |
Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109978 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503476501282816 |