Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.

Detalhes bibliográficos
Autor(a) principal: PEREIRA, J. F.
Data de Publicação: 2013
Outros Autores: ALMEIDA, A. P. M. M., COTA, J., PAMPHILE, J. A., SILVA, G. F. da, ARAÚJO, E. F. de, GRAMACHO, K. P., BROMMONSCHENKEL, S. H., PEREIRA, G. A. G., QUEIROZ, M. V. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
Resumo: Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.
id EMBR_e3cb71ca005ff48386b28ced6d6a001a
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/946075
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.Moniliophthora perniciosaBoto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA.PEREIRA, J. F.ALMEIDA, A. P. M. M.COTA, J.PAMPHILE, J. A.SILVA, G. F. daARAÚJO, E. F. deGRAMACHO, K. P.BROMMONSCHENKEL, S. H.PEREIRA, G. A. G.QUEIROZ, M. V. de2013-01-23T11:11:11Z2013-01-23T11:11:11Z2013-01-2320132013-06-24T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMicrobiology, v. 159, p. 112-125, 2013.http://www.alice.cnptia.embrapa.br/alice/handle/doc/94607510.1099/mic.0.062901-0enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:56:21Zoai:www.alice.cnptia.embrapa.br:doc/946075Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:56:21falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:56:21Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
spellingShingle Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
PEREIRA, J. F.
Moniliophthora perniciosa
title_short Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_full Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_fullStr Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_full_unstemmed Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_sort Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
author PEREIRA, J. F.
author_facet PEREIRA, J. F.
ALMEIDA, A. P. M. M.
COTA, J.
PAMPHILE, J. A.
SILVA, G. F. da
ARAÚJO, E. F. de
GRAMACHO, K. P.
BROMMONSCHENKEL, S. H.
PEREIRA, G. A. G.
QUEIROZ, M. V. de
author_role author
author2 ALMEIDA, A. P. M. M.
COTA, J.
PAMPHILE, J. A.
SILVA, G. F. da
ARAÚJO, E. F. de
GRAMACHO, K. P.
BROMMONSCHENKEL, S. H.
PEREIRA, G. A. G.
QUEIROZ, M. V. de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA.
dc.contributor.author.fl_str_mv PEREIRA, J. F.
ALMEIDA, A. P. M. M.
COTA, J.
PAMPHILE, J. A.
SILVA, G. F. da
ARAÚJO, E. F. de
GRAMACHO, K. P.
BROMMONSCHENKEL, S. H.
PEREIRA, G. A. G.
QUEIROZ, M. V. de
dc.subject.por.fl_str_mv Moniliophthora perniciosa
topic Moniliophthora perniciosa
description Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-23T11:11:11Z
2013-01-23T11:11:11Z
2013-01-23
2013
2013-06-24T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Microbiology, v. 159, p. 112-125, 2013.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
10.1099/mic.0.062901-0
identifier_str_mv Microbiology, v. 159, p. 112-125, 2013.
10.1099/mic.0.062901-0
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503374798848000