Humic acids induce the expression of nitrate transporters in passion-fruit seedlings.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153077 https://doi.org/10.1590/0100-29452023941 |
Resumo: | The management of organic fertilization in passion fruit has been carried out empirically based on the responses of the crops and the experience of farmers. Knowledge of the physiological responses of plants to organic fertilizers is essential to optimize fertilization programs. The objective of this study was to evaluate the differential expression of genes coding for nitrate transporters as well as plasma membrane H+-ATPase in passion fruit seedlings treated with different organic fertilizers in the presence or not of humic acids. The equivalent of one gram of total nitrogen dissolved in water from cattle manure, castor oil cake and urea were added and the seedlings were treated or not with a suspension of humic acids (2 mmol/L). Differential expression of low and high affinity nitrate transporters (PeNRT2.2 and PeNRT1.1 PeNRT2.2e) and H+-ATPase (PeMha) was performed by qRT-PCR. The use of cattle manure increased the differential expression of the high affinity transporter regarding to the control. The application of humic acids amplified the increase observed in the differential transcription of the high-affinity transporter with manure and urea fertilization, with no effect observed with the use of castor oil cake. Humic acids induced transcription of the gene encoding H+-ATPases in all treatments compared to control. The application of biofertilizer based on humic acids in low concentrations directly on the plant can be used with the objective of optimizing the organic fertilization of passion fruit with cattle manure and urea. |
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Humic acids induce the expression of nitrate transporters in passion-fruit seedlings.Physiological responsesNRT genesHATPaseOrganic fertilizersThe management of organic fertilization in passion fruit has been carried out empirically based on the responses of the crops and the experience of farmers. Knowledge of the physiological responses of plants to organic fertilizers is essential to optimize fertilization programs. The objective of this study was to evaluate the differential expression of genes coding for nitrate transporters as well as plasma membrane H+-ATPase in passion fruit seedlings treated with different organic fertilizers in the presence or not of humic acids. The equivalent of one gram of total nitrogen dissolved in water from cattle manure, castor oil cake and urea were added and the seedlings were treated or not with a suspension of humic acids (2 mmol/L). Differential expression of low and high affinity nitrate transporters (PeNRT2.2 and PeNRT1.1 PeNRT2.2e) and H+-ATPase (PeMha) was performed by qRT-PCR. The use of cattle manure increased the differential expression of the high affinity transporter regarding to the control. The application of humic acids amplified the increase observed in the differential transcription of the high-affinity transporter with manure and urea fertilization, with no effect observed with the use of castor oil cake. Humic acids induced transcription of the gene encoding H+-ATPases in all treatments compared to control. The application of biofertilizer based on humic acids in low concentrations directly on the plant can be used with the objective of optimizing the organic fertilization of passion fruit with cattle manure and urea.Título em Português: Ácidos húmicos induzem a expressão dos transportadores de nitrato em mudas de maracujazeiroPAULO FERNANDO MARQUES CAVALCANTI FILHO, UENF; DAIANE CARVALHO BAÍA, UENF; RAFAEL CHAVES RIBEIRO, UENF; RAUL CASTRO CARRIELLO ROSA, CNPAB; LUCIANO PASQUALOTO CANELLAS, UENF.CAVALCANTI FILHO, P. F. M.BAÍA, D. C.RIBEIRO, R. C.ROSA, R. C. C.CANELLAS, L. P.2023-04-11T16:03:50Z2023-04-11T16:03:50Z2023-04-112023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Fruticultura, v. 45, Article number e-941, 2023.1806-9967http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153077https://doi.org/10.1590/0100-29452023941enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-04-11T16:03:50Zoai:www.alice.cnptia.embrapa.br:doc/1153077Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-04-11T16:03:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-04-11T16:03:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
title |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
spellingShingle |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. CAVALCANTI FILHO, P. F. M. Physiological responses NRT genes HATPase Organic fertilizers |
title_short |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
title_full |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
title_fullStr |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
title_full_unstemmed |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
title_sort |
Humic acids induce the expression of nitrate transporters in passion-fruit seedlings. |
author |
CAVALCANTI FILHO, P. F. M. |
author_facet |
CAVALCANTI FILHO, P. F. M. BAÍA, D. C. RIBEIRO, R. C. ROSA, R. C. C. CANELLAS, L. P. |
author_role |
author |
author2 |
BAÍA, D. C. RIBEIRO, R. C. ROSA, R. C. C. CANELLAS, L. P. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
PAULO FERNANDO MARQUES CAVALCANTI FILHO, UENF; DAIANE CARVALHO BAÍA, UENF; RAFAEL CHAVES RIBEIRO, UENF; RAUL CASTRO CARRIELLO ROSA, CNPAB; LUCIANO PASQUALOTO CANELLAS, UENF. |
dc.contributor.author.fl_str_mv |
CAVALCANTI FILHO, P. F. M. BAÍA, D. C. RIBEIRO, R. C. ROSA, R. C. C. CANELLAS, L. P. |
dc.subject.por.fl_str_mv |
Physiological responses NRT genes HATPase Organic fertilizers |
topic |
Physiological responses NRT genes HATPase Organic fertilizers |
description |
The management of organic fertilization in passion fruit has been carried out empirically based on the responses of the crops and the experience of farmers. Knowledge of the physiological responses of plants to organic fertilizers is essential to optimize fertilization programs. The objective of this study was to evaluate the differential expression of genes coding for nitrate transporters as well as plasma membrane H+-ATPase in passion fruit seedlings treated with different organic fertilizers in the presence or not of humic acids. The equivalent of one gram of total nitrogen dissolved in water from cattle manure, castor oil cake and urea were added and the seedlings were treated or not with a suspension of humic acids (2 mmol/L). Differential expression of low and high affinity nitrate transporters (PeNRT2.2 and PeNRT1.1 PeNRT2.2e) and H+-ATPase (PeMha) was performed by qRT-PCR. The use of cattle manure increased the differential expression of the high affinity transporter regarding to the control. The application of humic acids amplified the increase observed in the differential transcription of the high-affinity transporter with manure and urea fertilization, with no effect observed with the use of castor oil cake. Humic acids induced transcription of the gene encoding H+-ATPases in all treatments compared to control. The application of biofertilizer based on humic acids in low concentrations directly on the plant can be used with the objective of optimizing the organic fertilization of passion fruit with cattle manure and urea. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-11T16:03:50Z 2023-04-11T16:03:50Z 2023-04-11 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Fruticultura, v. 45, Article number e-941, 2023. 1806-9967 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153077 https://doi.org/10.1590/0100-29452023941 |
identifier_str_mv |
Revista Brasileira de Fruticultura, v. 45, Article number e-941, 2023. 1806-9967 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153077 https://doi.org/10.1590/0100-29452023941 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503543062790144 |