A first look at the N- and O-glycosylation landscape in anuran skin secretions.

Detalhes bibliográficos
Autor(a) principal: BARBOSA, E. A.
Data de Publicação: 2022
Outros Autores: ALVES, G. S. C., COURA, M. de M. A., SILVA, H. de L. e, ROCHA, F. S. da, NUNES, J. B., WATANABE, M. de S., ANDRADE, A. C., BRANDA, G. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150624
https://doi.org/10.1016/j.biochi.2022.01.008
Resumo: Amphibians secrete a complex array of molecules that shape their interactions with coinhabiting microorganisms and macroscopic predators. Glycans are a rapidly evolving and complex class of biomolecules implicated in intrinsic and extrinsic recognition events. Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins. In the present report, LC-MS/MS was used to investigate the diversity of N- and O-linked oligosaccharides in the skin secretion of two South American frogs, Pithecopus azureus and Boana raniceps. Additionally, the enzymes responsible for glycan synthesis pathways were evaluated based on their skin tissue transcriptome. Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins. Paucimannosidic glycans were abundant in the skin secretion of both amphibians; however, hybrid and complex N-glycan structures were detected only in B. raniceps. A good correlation between the structures discovered in glycomic analyses and transcripts encoding enzymes necessary for their synthesis was obtained. Some transcripts such as those of MAN1A2, FUT8, and ST6GALNAC were found solely in B. raniceps. Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein. Overall, our results show the presence of oligosaccharides in amphibians skin secretions and suggest that their diversity is species-specific, paving the way for novel perspectives involving amphibian evolution and ecology.
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spelling A first look at the N- and O-glycosylation landscape in anuran skin secretions.Mass spectrometryTranscriptomicsOligosaccharidesAmphibians secrete a complex array of molecules that shape their interactions with coinhabiting microorganisms and macroscopic predators. Glycans are a rapidly evolving and complex class of biomolecules implicated in intrinsic and extrinsic recognition events. Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins. In the present report, LC-MS/MS was used to investigate the diversity of N- and O-linked oligosaccharides in the skin secretion of two South American frogs, Pithecopus azureus and Boana raniceps. Additionally, the enzymes responsible for glycan synthesis pathways were evaluated based on their skin tissue transcriptome. Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins. Paucimannosidic glycans were abundant in the skin secretion of both amphibians; however, hybrid and complex N-glycan structures were detected only in B. raniceps. A good correlation between the structures discovered in glycomic analyses and transcripts encoding enzymes necessary for their synthesis was obtained. Some transcripts such as those of MAN1A2, FUT8, and ST6GALNAC were found solely in B. raniceps. Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein. Overall, our results show the presence of oligosaccharides in amphibians skin secretions and suggest that their diversity is species-specific, paving the way for novel perspectives involving amphibian evolution and ecology.EDER ALVES BARBOSA, UNIVERSIDADE DE BRASÍLIA; GABRIEL SÉRGIO COSTA ALVES, UNIVERSIDADE DE BRASÍLIA; MARCELO DE MELO ANDRADE COURA, UNIVERSIDADE DE BRASÍLIA; HIGOR DE LIMA E SILVA, UNIVERSIDADE DE BRASÍLIA; FILIPE SOUZA DA ROCHA, UNIVERSIDADE DE BRASÍLIA; JOÃO BUENO NUNES, UNIVERSIDADE DE BRASÍLIA; MATHEUS DE SOUZA WATANABE, UNIVERSIDADE DE BRASÍLIA; ALAN CARVALHO ANDRADE, CNPCa; GUILHERME DOTTO BRANDA, UNIVERSIDADE DE BRASÍLIA.BARBOSA, E. A.ALVES, G. S. C.COURA, M. de M. A.SILVA, H. de L. eROCHA, F. S. daNUNES, J. B.WATANABE, M. de S.ANDRADE, A. C.BRANDA, G. D.2023-01-04T15:01:34Z2023-01-04T15:01:34Z2023-01-042022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiochimie, v. 197, Jun., p.19-37, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150624https://doi.org/10.1016/j.biochi.2022.01.008enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-01-04T15:01:34Zoai:www.alice.cnptia.embrapa.br:doc/1150624Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-01-04T15:01:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-01-04T15:01:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv A first look at the N- and O-glycosylation landscape in anuran skin secretions.
title A first look at the N- and O-glycosylation landscape in anuran skin secretions.
spellingShingle A first look at the N- and O-glycosylation landscape in anuran skin secretions.
BARBOSA, E. A.
Mass spectrometry
Transcriptomics
Oligosaccharides
title_short A first look at the N- and O-glycosylation landscape in anuran skin secretions.
title_full A first look at the N- and O-glycosylation landscape in anuran skin secretions.
title_fullStr A first look at the N- and O-glycosylation landscape in anuran skin secretions.
title_full_unstemmed A first look at the N- and O-glycosylation landscape in anuran skin secretions.
title_sort A first look at the N- and O-glycosylation landscape in anuran skin secretions.
author BARBOSA, E. A.
author_facet BARBOSA, E. A.
ALVES, G. S. C.
COURA, M. de M. A.
SILVA, H. de L. e
ROCHA, F. S. da
NUNES, J. B.
WATANABE, M. de S.
ANDRADE, A. C.
BRANDA, G. D.
author_role author
author2 ALVES, G. S. C.
COURA, M. de M. A.
SILVA, H. de L. e
ROCHA, F. S. da
NUNES, J. B.
WATANABE, M. de S.
ANDRADE, A. C.
BRANDA, G. D.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv EDER ALVES BARBOSA, UNIVERSIDADE DE BRASÍLIA; GABRIEL SÉRGIO COSTA ALVES, UNIVERSIDADE DE BRASÍLIA; MARCELO DE MELO ANDRADE COURA, UNIVERSIDADE DE BRASÍLIA; HIGOR DE LIMA E SILVA, UNIVERSIDADE DE BRASÍLIA; FILIPE SOUZA DA ROCHA, UNIVERSIDADE DE BRASÍLIA; JOÃO BUENO NUNES, UNIVERSIDADE DE BRASÍLIA; MATHEUS DE SOUZA WATANABE, UNIVERSIDADE DE BRASÍLIA; ALAN CARVALHO ANDRADE, CNPCa; GUILHERME DOTTO BRANDA, UNIVERSIDADE DE BRASÍLIA.
dc.contributor.author.fl_str_mv BARBOSA, E. A.
ALVES, G. S. C.
COURA, M. de M. A.
SILVA, H. de L. e
ROCHA, F. S. da
NUNES, J. B.
WATANABE, M. de S.
ANDRADE, A. C.
BRANDA, G. D.
dc.subject.por.fl_str_mv Mass spectrometry
Transcriptomics
Oligosaccharides
topic Mass spectrometry
Transcriptomics
Oligosaccharides
description Amphibians secrete a complex array of molecules that shape their interactions with coinhabiting microorganisms and macroscopic predators. Glycans are a rapidly evolving and complex class of biomolecules implicated in intrinsic and extrinsic recognition events. Despite the numerous studies aiming at the biochemical characterization of anuran skin secretions, little is known about protein-linked oligosaccharides, their synthesis pathways, and their homing secreted glycoproteins. In the present report, LC-MS/MS was used to investigate the diversity of N- and O-linked oligosaccharides in the skin secretion of two South American frogs, Pithecopus azureus and Boana raniceps. Additionally, the enzymes responsible for glycan synthesis pathways were evaluated based on their skin tissue transcriptome. Our analyses allowed the annotation of various N- and O-glycan structures commonly found in vertebrate proteins. Paucimannosidic glycans were abundant in the skin secretion of both amphibians; however, hybrid and complex N-glycan structures were detected only in B. raniceps. A good correlation between the structures discovered in glycomic analyses and transcripts encoding enzymes necessary for their synthesis was obtained. Some transcripts such as those of MAN1A2, FUT8, and ST6GALNAC were found solely in B. raniceps. Finally, secreted N- and O- linked glycoproteins were predicted from the transcriptomic data, indicating that proteases and protease inhibitors are putative sources of the glycans described herein. Overall, our results show the presence of oligosaccharides in amphibians skin secretions and suggest that their diversity is species-specific, paving the way for novel perspectives involving amphibian evolution and ecology.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023-01-04T15:01:34Z
2023-01-04T15:01:34Z
2023-01-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Biochimie, v. 197, Jun., p.19-37, 2022.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150624
https://doi.org/10.1016/j.biochi.2022.01.008
identifier_str_mv Biochimie, v. 197, Jun., p.19-37, 2022.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1150624
https://doi.org/10.1016/j.biochi.2022.01.008
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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