Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/489109 |
Resumo: | The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 and 65% of the phenotypic variation for these traits among the isolates, respectively.. |
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Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD.Lagarta do cartuchoBactériaZea MaysThe total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 and 65% of the phenotypic variation for these traits among the isolates, respectively..CLAUDIA TEIXEIRA GUIMARAES, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS.BARRETO, M. R.GUIMARAES, C. T.TEIXEIRA, F. F.PAIVA, E.VALICENTE, F. H.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-04-10T11:11:11Z2006-01-2020052018-05-30T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005.http://www.alice.cnptia.embrapa.br/alice/handle/doc/489109enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:20:35Zoai:www.alice.cnptia.embrapa.br:doc/489109Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:20:35falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:20:35Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
title |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
spellingShingle |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. BARRETO, M. R. Lagarta do cartucho Bactéria Zea Mays |
title_short |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
title_full |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
title_fullStr |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
title_full_unstemmed |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
title_sort |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
author |
BARRETO, M. R. |
author_facet |
BARRETO, M. R. GUIMARAES, C. T. TEIXEIRA, F. F. PAIVA, E. VALICENTE, F. H. |
author_role |
author |
author2 |
GUIMARAES, C. T. TEIXEIRA, F. F. PAIVA, E. VALICENTE, F. H. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS. |
dc.contributor.author.fl_str_mv |
BARRETO, M. R. GUIMARAES, C. T. TEIXEIRA, F. F. PAIVA, E. VALICENTE, F. H. |
dc.subject.por.fl_str_mv |
Lagarta do cartucho Bactéria Zea Mays |
topic |
Lagarta do cartucho Bactéria Zea Mays |
description |
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 and 65% of the phenotypic variation for these traits among the isolates, respectively.. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2006-01-20 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2018-05-30T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Neotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005. http://www.alice.cnptia.embrapa.br/alice/handle/doc/489109 |
identifier_str_mv |
Neotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/489109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503354212155392 |