Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148633 |
Resumo: | Fungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation. |
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Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods.MALDI-MSIBiotecnologiaAspergillus terreusPleurotus pulmonariusMetabolomicsBiotechnologyFungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation.TALLYTA SANTOS TEIXEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; JORGE CANDIDO RODRIGUES NETO, UNIVERSIDADE FEDERAL DE GOIÁS; ELIAS ALVES SILVA, CNPAE; APARECIDO ALMEIDA CONCEIÇÃO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE.TEIXEIRA, T. S.RODRIGUES NETO, J. C.SILVA, E. A.CONCEIÇÃO, A. A.SIQUEIRA, F. G. deABDELNUR, P. V.2023-02-13T13:01:40Z2023-02-13T13:01:40Z2022-11-232023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/114863310.30744/brjac.2179-3425.AR-44-2022enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-02-13T13:01:40Zoai:www.alice.cnptia.embrapa.br:doc/1148633Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-02-13T13:01:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-02-13T13:01:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
title |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
spellingShingle |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. TEIXEIRA, T. S. MALDI-MSI Biotecnologia Aspergillus terreus Pleurotus pulmonarius Metabolomics Biotechnology |
title_short |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
title_full |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
title_fullStr |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
title_full_unstemmed |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
title_sort |
Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. |
author |
TEIXEIRA, T. S. |
author_facet |
TEIXEIRA, T. S. RODRIGUES NETO, J. C. SILVA, E. A. CONCEIÇÃO, A. A. SIQUEIRA, F. G. de ABDELNUR, P. V. |
author_role |
author |
author2 |
RODRIGUES NETO, J. C. SILVA, E. A. CONCEIÇÃO, A. A. SIQUEIRA, F. G. de ABDELNUR, P. V. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
TALLYTA SANTOS TEIXEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; JORGE CANDIDO RODRIGUES NETO, UNIVERSIDADE FEDERAL DE GOIÁS; ELIAS ALVES SILVA, CNPAE; APARECIDO ALMEIDA CONCEIÇÃO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
dc.contributor.author.fl_str_mv |
TEIXEIRA, T. S. RODRIGUES NETO, J. C. SILVA, E. A. CONCEIÇÃO, A. A. SIQUEIRA, F. G. de ABDELNUR, P. V. |
dc.subject.por.fl_str_mv |
MALDI-MSI Biotecnologia Aspergillus terreus Pleurotus pulmonarius Metabolomics Biotechnology |
topic |
MALDI-MSI Biotecnologia Aspergillus terreus Pleurotus pulmonarius Metabolomics Biotechnology |
description |
Fungi can produce many bioactive metabolites, which are enhanced when challenged in co-culture competition. For a better evaluation of these metabolites, Mass Spectrometry Imaging (MSI) can be used to provide complementary information about the metabolite spatial localization. However, some adaptations are required on available methodologies in MSI for applications in microorganisms, particularly on sample preparation, due to the characteristics of each type of cell that has to be analyzed. The imprinting method has been shown to be a robust method when applied to sample preparation, but to our knowledge it has never been tested for microbial MALDI-MSI. Herein we applied both Classic and Imprinting MALDIMSI to compare and analyze metabolites produced by Aspergillus terreus (ATCC® 20542TM) and Pleurotus pulmonarius fungi. For the classic method, the fungi were inoculated for 8 days with PDA medium in a MALDI glass slide. For the imprinting method, fungi were also inoculated for 8 days in a MALDI glass slide and then transferred to a filter paper by manual pressure using a homemade apparatus. Samples were then dehydrated and submitted to HCCA matrix application by sublimation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-23 2023-02-13T13:01:40Z 2023-02-13T13:01:40Z 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148633 10.30744/brjac.2179-3425.AR-44-2022 |
identifier_str_mv |
Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023. 10.30744/brjac.2179-3425.AR-44-2022 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148633 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503539572080640 |