Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111 |
Resumo: | In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation. |
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Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli.Capsicum frutescens cv StromboliCélula foliarMetabólicos secundáriosProtocolo de diferenciação celularLeaf cellsCallogenesisGrowth curvesDeceleration phaseFase de desaceleraçãoEmbrapa RondôniaPorto Velho (RO)RondôniaAmazônia OcidentalWestern AmazonCultura In VitroCalogêneseCurva de CrescimentoIn vitro cultureCallus formationSecondary metabolitesCell dedifferentiationIn vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation.MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia.SANTOS, M. R. A. dosPAZ, E. S.2020-11-27T09:04:16Z2020-11-27T09:04:16Z2020-11-262020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020.2230-9926http://www.alice.cnptia.embrapa.br/alice/handle/doc/112711110.37118/ijdr.20362.11.2020enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-11-27T09:04:22Zoai:www.alice.cnptia.embrapa.br:doc/1127111Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-11-27T09:04:22falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-11-27T09:04:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
title |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
spellingShingle |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. SANTOS, M. R. A. dos Capsicum frutescens cv Stromboli Célula foliar Metabólicos secundários Protocolo de diferenciação celular Leaf cells Callogenesis Growth curves Deceleration phase Fase de desaceleração Embrapa Rondônia Porto Velho (RO) Rondônia Amazônia Ocidental Western Amazon Cultura In Vitro Calogênese Curva de Crescimento In vitro culture Callus formation Secondary metabolites Cell dedifferentiation |
title_short |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
title_full |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
title_fullStr |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
title_full_unstemmed |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
title_sort |
Dedifferentation of leaf cells and growth pattern of caluses of Capsicum frutescens cv. Stromboli. |
author |
SANTOS, M. R. A. dos |
author_facet |
SANTOS, M. R. A. dos PAZ, E. S. |
author_role |
author |
author2 |
PAZ, E. S. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO; Universidade Federal de Rondonia. |
dc.contributor.author.fl_str_mv |
SANTOS, M. R. A. dos PAZ, E. S. |
dc.subject.por.fl_str_mv |
Capsicum frutescens cv Stromboli Célula foliar Metabólicos secundários Protocolo de diferenciação celular Leaf cells Callogenesis Growth curves Deceleration phase Fase de desaceleração Embrapa Rondônia Porto Velho (RO) Rondônia Amazônia Ocidental Western Amazon Cultura In Vitro Calogênese Curva de Crescimento In vitro culture Callus formation Secondary metabolites Cell dedifferentiation |
topic |
Capsicum frutescens cv Stromboli Célula foliar Metabólicos secundários Protocolo de diferenciação celular Leaf cells Callogenesis Growth curves Deceleration phase Fase de desaceleração Embrapa Rondônia Porto Velho (RO) Rondônia Amazônia Ocidental Western Amazon Cultura In Vitro Calogênese Curva de Crescimento In vitro culture Callus formation Secondary metabolites Cell dedifferentiation |
description |
In vitro cell suspension cultivation systems have been largely reported as safe and standardized methods of production of secondary metabolites with medicinal and agricultural interest. Capsicum frutescensis one of the most widely grown vegetable in the world and its biological activities have been demonstrated against insects, fungi, bacteria and other groups of organisms. The objective of this study was to establish a protocol for dedifferentiation of leaf cells of the cultivar C. frutescens cv. Stromboli and to determine the growth pattern of the calluses with a focus on the deceleration phase, when the callus cells must be subcultured into a liquid medium in order to establish cell suspension cultivations aiming at the production of secondary metabolites. The explants were inoculated into a medium supplemented with BA and 2,4-D in factorial combinations. The percentage of callus induction (%CI), the explant area covered by callus cells (ACCC) and the fresh weight of the calluses were evaluated. The procedures that resulted in higher proliferation of callus cells were repeated in order to determine the growth curve of the calluses. The treatment that resulted in the highest %CI, ACCC and callus weight was 1.5 mg L-1 2,4-D. The calluses produced were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 39th day of cultivation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-27T09:04:16Z 2020-11-27T09:04:16Z 2020-11-26 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020. 2230-9926 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111 10.37118/ijdr.20362.11.2020 |
identifier_str_mv |
International Journal of Development Research, v. 10, n. 11, p. 41853-41856, Nov. 2020. 2230-9926 10.37118/ijdr.20362.11.2020 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503498252943360 |