In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid

Detalhes bibliográficos
Autor(a) principal: da Silva, Simone
Data de Publicação: 2018
Outros Autores: Pinheiro, Ester Neta, Assunção, Laís Medeiros, Silva, Efigênia Lopes, Rodrigues, Daniele Carvalho, Ferreira, Flávio Freires, Marinho, Vitor Rafael Pereira, Astolfi-Filho, Spartaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Fitos
Texto Completo: https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620
Resumo: Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation.
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spelling In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic AcidPropagação in vitro de Psychotria ipecacuanha (Brot.) Stokes sob a ação de diferentes concentrações de Ácido IndolacéticoPlant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac.Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation.Psychotria ipecacuanha, é uma planta medicinal nativa da América com a maior área de ocorrência no Estado de Mato Grosso - Brasil, críticamente ameaçada devido à sobreexploração das populações naturais. Devido à dificuldades na propagação convencional, o objetivo deste estudo foi a avaliação do efeito de diferentes concentrações do regulador de crescimento ácido indolacético (AIA) na propagação in vitro de P. ipecacuanha. Segmentos nodais foram cultivados em meio de Murashige e Skoog (MS) sem reguladores de crescimento (controle) e suplementado com quatro concentrações (0,05; 0,5; 1,5 e 2,0 mg.L-1) de AIA, em meios semi-sólidos. Após 60 dias de cultivo, segmentos nodais (n=30) cultivados em meio de Murashige & Skoog (MS) suplementado com 0,05 mg.L-1 de AIA produziram, em média, 4,56 segmentos nodais por explante, cujas plântulas foram aclimatizadas com sucesso, sem exibição de qualquer anomalia morfológica ou variação.Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz2018-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/62010.17648/2446-4775.2018.620Revista Fitos; Vol. 12 No. 3 (2018); 263-268Revista Fitos; Vol. 12 Núm. 3 (2018); 263-268Revista Fitos; v. 12 n. 3 (2018); 263-2682446-47751808-9569reponame:Revista Fitosinstname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZenghttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/pdfhttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/htmlCopyright (c) 2018 Revista Fitos Eletrônicainfo:eu-repo/semantics/openAccessda Silva, SimonePinheiro, Ester NetaAssunção, Laís MedeirosSilva, Efigênia LopesRodrigues, Daniele CarvalhoFerreira, Flávio FreiresMarinho, Vitor Rafael PereiraAstolfi-Filho, Spartaco2021-01-29T13:21:14Zoai:ojs.revistafitos.far.fiocruz.br:article/620Revistahttps://revistafitos.far.fiocruz.br/index.php/revista-fitos/PUBhttp://revistafitos.far.fiocruz.br/index.php/revista-fitos/oairevistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br2446-47752446-4775opendoar:2021-01-29T13:21:14Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.none.fl_str_mv In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
Propagação in vitro de Psychotria ipecacuanha (Brot.) Stokes sob a ação de diferentes concentrações de Ácido Indolacético
title In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
spellingShingle In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
da Silva, Simone
Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac.
title_short In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
title_full In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
title_fullStr In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
title_full_unstemmed In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
title_sort In vitro propagation of Psychotria ipecacuanha (Brot.) Stokes under different concentrations of Indoleacetic Acid
author da Silva, Simone
author_facet da Silva, Simone
Pinheiro, Ester Neta
Assunção, Laís Medeiros
Silva, Efigênia Lopes
Rodrigues, Daniele Carvalho
Ferreira, Flávio Freires
Marinho, Vitor Rafael Pereira
Astolfi-Filho, Spartaco
author_role author
author2 Pinheiro, Ester Neta
Assunção, Laís Medeiros
Silva, Efigênia Lopes
Rodrigues, Daniele Carvalho
Ferreira, Flávio Freires
Marinho, Vitor Rafael Pereira
Astolfi-Filho, Spartaco
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv da Silva, Simone
Pinheiro, Ester Neta
Assunção, Laís Medeiros
Silva, Efigênia Lopes
Rodrigues, Daniele Carvalho
Ferreira, Flávio Freires
Marinho, Vitor Rafael Pereira
Astolfi-Filho, Spartaco
dc.subject.por.fl_str_mv Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac.
topic Plant biotechnology. tissue culture. micropropagation. Rubiaceae. ipecac.
description Psychotria ipecacuanha is a medicinal plant that is native to the Americas with the largest area of occurrence in the State of Mato Grosso in Brazil. It is critically endangered due to overexploitation of natural populations. Due to difficulties in conventional propagation, the aim of this study was to evaluate the effect of different concentrations of the growth regulator indoleacetic acid (IAA) on in vitro propagation of this species. Stem node explants were cultured in Murashige and Skoog medium (MS) without growth regulators (control) and supplemented with four concentrations (0.05, 0.5, 1.5 and 2.0 mg.L-1) of indoleacetic acid (IAA) in semisolid media. After 60 days of cultivation, nodal segments (n=30) grown in Murashige & Skoog (MS) medium supplemented with 0.05 mg.L-1 of IAA produced on average 4.56 nodal segments per explant. The seedlings were successfully acclimatized without detection of any morphological anomalies or variation.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620
10.17648/2446-4775.2018.620
url https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620
identifier_str_mv 10.17648/2446-4775.2018.620
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/pdf
https://revistafitos.far.fiocruz.br/index.php/revista-fitos/article/view/620/html
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista Fitos Eletrônica
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista Fitos Eletrônica
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
publisher.none.fl_str_mv Centro de Inovação em Biodiversidade e Saúde - Farmanguinhos/Fiocruz
dc.source.none.fl_str_mv Revista Fitos; Vol. 12 No. 3 (2018); 263-268
Revista Fitos; Vol. 12 Núm. 3 (2018); 263-268
Revista Fitos; v. 12 n. 3 (2018); 263-268
2446-4775
1808-9569
reponame:Revista Fitos
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Revista Fitos
collection Revista Fitos
repository.name.fl_str_mv Revista Fitos - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv revistafitos@far.fiocruz.br || eugenio.telles@far.fiocruz.br
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