The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins

Detalhes bibliográficos
Autor(a) principal: Ramos,Márcio Viana
Data de Publicação: 1996
Outros Autores: Moreira,Renato de Azevedo, Oliveira,José Tadeu Abreu, Cavada,Benildo Sousa, Rougé,Pierre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600021
Resumo: The carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously reported for other lectins from Diocleinae (tribe Phaseoleae, sub-tribe Diocleinae). Accordingly, both lectins exhibited almost identical hydropathic profiles and their three-dimensional models built up from the atomic coordinates of ConA looked very similar. However, docking experiments of glucose and mannose in their monosaccharide-binding sites, by comparison with the ConA-mannose complex used as a model, revealed conformational changes in side chains of the amino acid residues involved in the binding of monosaccharides. These results fully agree with crystallographic data showing that binding of specific ligands to ConA requires conformational chances of its monosaccharide-binding site.
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spelling The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectinsCanavalia maritimacarbohydrate-binding specificityDiocleinaeDioclea grandifloralectinsmolecular modellingThe carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously reported for other lectins from Diocleinae (tribe Phaseoleae, sub-tribe Diocleinae). Accordingly, both lectins exhibited almost identical hydropathic profiles and their three-dimensional models built up from the atomic coordinates of ConA looked very similar. However, docking experiments of glucose and mannose in their monosaccharide-binding sites, by comparison with the ConA-mannose complex used as a model, revealed conformational changes in side chains of the amino acid residues involved in the binding of monosaccharides. These results fully agree with crystallographic data showing that binding of specific ligands to ConA requires conformational chances of its monosaccharide-binding site.Instituto Oswaldo Cruz, Ministério da Saúde1996-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600021Memórias do Instituto Oswaldo Cruz v.91 n.6 1996reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761996000600021info:eu-repo/semantics/openAccessRamos,Márcio VianaMoreira,Renato de AzevedoOliveira,José Tadeu AbreuCavada,Benildo SousaRougé,Pierreeng2020-04-25T17:47:36Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:14.97Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
title The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
spellingShingle The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
Ramos,Márcio Viana
Canavalia maritima
carbohydrate-binding specificity
Diocleinae
Dioclea grandiflora
lectins
molecular modelling
title_short The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
title_full The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
title_fullStr The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
title_full_unstemmed The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
title_sort The carbohydrate-binding specificity and molecular modelling of Canavalia maritima and Dioclea grandiflora lectins
author Ramos,Márcio Viana
author_facet Ramos,Márcio Viana
Moreira,Renato de Azevedo
Oliveira,José Tadeu Abreu
Cavada,Benildo Sousa
Rougé,Pierre
author_role author
author2 Moreira,Renato de Azevedo
Oliveira,José Tadeu Abreu
Cavada,Benildo Sousa
Rougé,Pierre
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ramos,Márcio Viana
Moreira,Renato de Azevedo
Oliveira,José Tadeu Abreu
Cavada,Benildo Sousa
Rougé,Pierre
dc.subject.por.fl_str_mv Canavalia maritima
carbohydrate-binding specificity
Diocleinae
Dioclea grandiflora
lectins
molecular modelling
topic Canavalia maritima
carbohydrate-binding specificity
Diocleinae
Dioclea grandiflora
lectins
molecular modelling
dc.description.none.fl_txt_mv The carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously reported for other lectins from Diocleinae (tribe Phaseoleae, sub-tribe Diocleinae). Accordingly, both lectins exhibited almost identical hydropathic profiles and their three-dimensional models built up from the atomic coordinates of ConA looked very similar. However, docking experiments of glucose and mannose in their monosaccharide-binding sites, by comparison with the ConA-mannose complex used as a model, revealed conformational changes in side chains of the amino acid residues involved in the binding of monosaccharides. These results fully agree with crystallographic data showing that binding of specific ligands to ConA requires conformational chances of its monosaccharide-binding site.
description The carbohydrate-binding specificity of lectins from the seeds of Canavalia maritima and Dioclea grandiflora was studied by hapten-inhibition of haemagglutination using various sugars and sugar derivatives as inhibitors, including N-acetylneuraminic acid and N-acetylmuramic acid. Despite some discrepancies, both lectins exhibited a very similar carbohydrate-binding specificity as previously reported for other lectins from Diocleinae (tribe Phaseoleae, sub-tribe Diocleinae). Accordingly, both lectins exhibited almost identical hydropathic profiles and their three-dimensional models built up from the atomic coordinates of ConA looked very similar. However, docking experiments of glucose and mannose in their monosaccharide-binding sites, by comparison with the ConA-mannose complex used as a model, revealed conformational changes in side chains of the amino acid residues involved in the binding of monosaccharides. These results fully agree with crystallographic data showing that binding of specific ligands to ConA requires conformational chances of its monosaccharide-binding site.
publishDate 1996
dc.date.none.fl_str_mv 1996-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600021
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761996000600021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761996000600021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.91 n.6 1996
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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