Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates

Detalhes bibliográficos
Autor(a) principal: Vilardo,Mônica de Castro Britto
Data de Publicação: 2006
Outros Autores: Thomé,Jacqueline Darc da Silva, Esteves,Wagner Thadeu Cardoso, Filgueiras,Ana Luzia Lauria, Oliveira,Selma Soares de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500003
Resumo: A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
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spelling Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primatesCampylobacteridentificationnon-human primatesA multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.Instituto Oswaldo Cruz, Ministério da Saúde2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500003Memórias do Instituto Oswaldo Cruz v.101 n.5 2006reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762006000500003info:eu-repo/semantics/openAccessVilardo,Mônica de Castro BrittoThomé,Jacqueline Darc da SilvaEsteves,Wagner Thadeu CardosoFilgueiras,Ana Luzia LauriaOliveira,Selma Soares deeng2020-04-25T17:49:36Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:13:53.071Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
spellingShingle Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Vilardo,Mônica de Castro Britto
Campylobacter
identification
non-human primates
title_short Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_full Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_fullStr Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_full_unstemmed Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
title_sort Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
author Vilardo,Mônica de Castro Britto
author_facet Vilardo,Mônica de Castro Britto
Thomé,Jacqueline Darc da Silva
Esteves,Wagner Thadeu Cardoso
Filgueiras,Ana Luzia Lauria
Oliveira,Selma Soares de
author_role author
author2 Thomé,Jacqueline Darc da Silva
Esteves,Wagner Thadeu Cardoso
Filgueiras,Ana Luzia Lauria
Oliveira,Selma Soares de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vilardo,Mônica de Castro Britto
Thomé,Jacqueline Darc da Silva
Esteves,Wagner Thadeu Cardoso
Filgueiras,Ana Luzia Lauria
Oliveira,Selma Soares de
dc.subject.por.fl_str_mv Campylobacter
identification
non-human primates
topic Campylobacter
identification
non-human primates
dc.description.none.fl_txt_mv A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
description A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
publishDate 2006
dc.date.none.fl_str_mv 2006-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500003
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000500003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762006000500003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.101 n.5 2006
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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