Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
Main Author: | |
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Publication Date: | 2011 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Memórias do Instituto Oswaldo Cruz |
Download full: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014 |
Summary: | Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil. |
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Neutralising antibodies for West Nile virus in horses from Brazilian PantanalWest Nile virusPantanalneutralisationequinescaimansBrazilFinancial support/CNPq, FAPERJ, FIOCRUZDespite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.Instituto Oswaldo Cruz, Ministério da Saúde2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014Memórias do Instituto Oswaldo Cruz v.106 n.4 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000400014info:eu-repo/semantics/openAccessPauvolid-Corrêa,AlexMorales,Maria AlejandraLevis,SilvanaFigueiredo,Luis Tadeu MoraesCouto-Lima,DinairCampos,ZilcaNogueira,Marcia FurlanSilva,Edson Elias daNogueira,Rita Maria RibeiroSchatzmayr,Hermann Gonçalveseng2020-04-25T17:51:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:41.834Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
title |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
spellingShingle |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal Pauvolid-Corrêa,Alex West Nile virus Pantanal neutralisation equines caimans BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ |
title_short |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
title_full |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
title_fullStr |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
title_full_unstemmed |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
title_sort |
Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal |
author |
Pauvolid-Corrêa,Alex |
author_facet |
Pauvolid-Corrêa,Alex Morales,Maria Alejandra Levis,Silvana Figueiredo,Luis Tadeu Moraes Couto-Lima,Dinair Campos,Zilca Nogueira,Marcia Furlan Silva,Edson Elias da Nogueira,Rita Maria Ribeiro Schatzmayr,Hermann Gonçalves |
author_role |
author |
author2 |
Morales,Maria Alejandra Levis,Silvana Figueiredo,Luis Tadeu Moraes Couto-Lima,Dinair Campos,Zilca Nogueira,Marcia Furlan Silva,Edson Elias da Nogueira,Rita Maria Ribeiro Schatzmayr,Hermann Gonçalves |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pauvolid-Corrêa,Alex Morales,Maria Alejandra Levis,Silvana Figueiredo,Luis Tadeu Moraes Couto-Lima,Dinair Campos,Zilca Nogueira,Marcia Furlan Silva,Edson Elias da Nogueira,Rita Maria Ribeiro Schatzmayr,Hermann Gonçalves |
dc.subject.por.fl_str_mv |
West Nile virus Pantanal neutralisation equines caimans BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ |
topic |
West Nile virus Pantanal neutralisation equines caimans BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ |
dc.description.none.fl_txt_mv |
Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil. |
description |
Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762011000400014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.106 n.4 2011 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
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Memórias do Instituto Oswaldo Cruz |
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Memórias do Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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FIOCRUZ |
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Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
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