Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal

Bibliographic Details
Main Author: Pauvolid-Corrêa,Alex
Publication Date: 2011
Other Authors: Morales,Maria Alejandra, Levis,Silvana, Figueiredo,Luis Tadeu Moraes, Couto-Lima,Dinair, Campos,Zilca, Nogueira,Marcia Furlan, Silva,Edson Elias da, Nogueira,Rita Maria Ribeiro, Schatzmayr,Hermann Gonçalves
Format: Article
Language: eng
Source: Memórias do Instituto Oswaldo Cruz
Download full: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014
Summary: Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
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spelling Neutralising antibodies for West Nile virus in horses from Brazilian PantanalWest Nile virusPantanalneutralisationequinescaimansBrazilFinancial support/CNPq, FAPERJ, FIOCRUZDespite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.Instituto Oswaldo Cruz, Ministério da Saúde2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014Memórias do Instituto Oswaldo Cruz v.106 n.4 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000400014info:eu-repo/semantics/openAccessPauvolid-Corrêa,AlexMorales,Maria AlejandraLevis,SilvanaFigueiredo,Luis Tadeu MoraesCouto-Lima,DinairCampos,ZilcaNogueira,Marcia FurlanSilva,Edson Elias daNogueira,Rita Maria RibeiroSchatzmayr,Hermann Gonçalveseng2020-04-25T17:51:01Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:41.834Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
title Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
spellingShingle Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
Pauvolid-Corrêa,Alex
West Nile virus
Pantanal
neutralisation
equines
caimans
BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ
title_short Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
title_full Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
title_fullStr Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
title_full_unstemmed Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
title_sort Neutralising antibodies for West Nile virus in horses from Brazilian Pantanal
author Pauvolid-Corrêa,Alex
author_facet Pauvolid-Corrêa,Alex
Morales,Maria Alejandra
Levis,Silvana
Figueiredo,Luis Tadeu Moraes
Couto-Lima,Dinair
Campos,Zilca
Nogueira,Marcia Furlan
Silva,Edson Elias da
Nogueira,Rita Maria Ribeiro
Schatzmayr,Hermann Gonçalves
author_role author
author2 Morales,Maria Alejandra
Levis,Silvana
Figueiredo,Luis Tadeu Moraes
Couto-Lima,Dinair
Campos,Zilca
Nogueira,Marcia Furlan
Silva,Edson Elias da
Nogueira,Rita Maria Ribeiro
Schatzmayr,Hermann Gonçalves
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pauvolid-Corrêa,Alex
Morales,Maria Alejandra
Levis,Silvana
Figueiredo,Luis Tadeu Moraes
Couto-Lima,Dinair
Campos,Zilca
Nogueira,Marcia Furlan
Silva,Edson Elias da
Nogueira,Rita Maria Ribeiro
Schatzmayr,Hermann Gonçalves
dc.subject.por.fl_str_mv West Nile virus
Pantanal
neutralisation
equines
caimans
BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ
topic West Nile virus
Pantanal
neutralisation
equines
caimans
BrazilFinancial support/CNPq, FAPERJ, FIOCRUZ
dc.description.none.fl_txt_mv Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
description Despite evidence of West Nile virus (WNV) activity in Colombia, Venezuela and Argentina, this virus has not been reported in most South American countries. In February 2009, we commenced an investigation for WNV in mosquitoes, horses and caimans from the Pantanal, Central-West Brazil. The sera of 168 horses and 30 caimans were initially tested using a flaviviruses-specific epitope-blocking enzyme-linked immunosorbent assay (blocking ELISA) for the detection of flavivirus-reactive antibodies. The seropositive samples were further tested using a plaque-reduction neutralisation test (PRNT90) for WNV and its most closely-related flaviviruses that circulate in Brazil to confirm the detection of specific virus-neutralising antibodies. Of the 93 (55.4%) blocking ELISA-seropositive horse serum samples, five (3%) were seropositive for WNV, nine (5.4%) were seropositive for St. Louis encephalitis virus, 18 (10.7%) were seropositive for Ilheus virus, three (1.8%) were seropositive for Cacipacore virus and none were seropositive for Rocio virus using PRNT90, with a criteria of > four-fold antibody titre difference. All caimans were negative for flaviviruses-specific antibodies using the blocking ELISA. No virus genome was detected from caiman blood or mosquito samples. The present study is the first report of confirmed serological evidence of WNV activity in Brazil.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000400014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.4 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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