Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection

Detalhes bibliográficos
Autor(a) principal: Camandaroba,Edson LP
Data de Publicação: 2006
Outros Autores: Reis,Eliana AG, Reis,Mitermayer G, Andrade,Sonia G
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000600005
Resumo: Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
id FIOCRUZ-4_2678375c63ffdfbdd81fe206bb6a42b9
oai_identifier_str oai:scielo:S0074-02762006000600005
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infectionTrypanosoma cruziColombian strain clonesschizodemeColombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.Instituto Oswaldo Cruz, Ministério da Saúde2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000600005Memórias do Instituto Oswaldo Cruz v.101 n.6 2006reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762006000600005info:eu-repo/semantics/openAccessCamandaroba,Edson LPReis,Eliana AGReis,Mitermayer GAndrade,Sonia Geng2020-04-25T17:49:38Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:13:56.437Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
spellingShingle Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
Camandaroba,Edson LP
Trypanosoma cruzi
Colombian strain clones
schizodeme
title_short Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_full Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_fullStr Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_full_unstemmed Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
title_sort Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
author Camandaroba,Edson LP
author_facet Camandaroba,Edson LP
Reis,Eliana AG
Reis,Mitermayer G
Andrade,Sonia G
author_role author
author2 Reis,Eliana AG
Reis,Mitermayer G
Andrade,Sonia G
author2_role author
author
author
dc.contributor.author.fl_str_mv Camandaroba,Edson LP
Reis,Eliana AG
Reis,Mitermayer G
Andrade,Sonia G
dc.subject.por.fl_str_mv Trypanosoma cruzi
Colombian strain clones
schizodeme
topic Trypanosoma cruzi
Colombian strain clones
schizodeme
dc.description.none.fl_txt_mv Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
description Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000600005
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000600005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762006000600005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.101 n.6 2006
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937697083359232

Registros relacionados