Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle

Detalhes bibliográficos
Autor(a) principal: Ruiz,Patrícia M Gonçalves
Data de Publicação: 2001
Outros Autores: Passos,Lygia MF, Machado,Rosangela Z, Lima,José D, Ribeiro,Múcio FB
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200017
Resumo: A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.
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spelling Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattleBabesia bigeminaELISAIgMA crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.Instituto Oswaldo Cruz, Ministério da Saúde2001-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200017Memórias do Instituto Oswaldo Cruz v.96 n.2 2001reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762001000200017info:eu-repo/semantics/openAccessRuiz,Patrícia M GonçalvesPassos,Lygia MFMachado,Rosangela ZLima,José DRibeiro,Múcio FBeng2020-04-25T17:48:31Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:10:11.271Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
title Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
spellingShingle Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
Ruiz,Patrícia M Gonçalves
Babesia bigemina
ELISA
IgM
title_short Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
title_full Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
title_fullStr Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
title_full_unstemmed Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
title_sort Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
author Ruiz,Patrícia M Gonçalves
author_facet Ruiz,Patrícia M Gonçalves
Passos,Lygia MF
Machado,Rosangela Z
Lima,José D
Ribeiro,Múcio FB
author_role author
author2 Passos,Lygia MF
Machado,Rosangela Z
Lima,José D
Ribeiro,Múcio FB
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ruiz,Patrícia M Gonçalves
Passos,Lygia MF
Machado,Rosangela Z
Lima,José D
Ribeiro,Múcio FB
dc.subject.por.fl_str_mv Babesia bigemina
ELISA
IgM
topic Babesia bigemina
ELISA
IgM
dc.description.none.fl_txt_mv A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.
description A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.
publishDate 2001
dc.date.none.fl_str_mv 2001-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762001000200017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.96 n.2 2001
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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