First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051 |
Resumo: | This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management. |
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Memórias do Instituto Oswaldo Cruz |
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First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributesCandida nivariensisBrazilantifungal resistancehydrolytic enzymesadherencebiofilmThis study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management.Instituto Oswaldo Cruz, Ministério da Saúde2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051Memórias do Instituto Oswaldo Cruz v.111 n.1 2016reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-02760150376info:eu-repo/semantics/openAccessFigueiredo-Carvalho,Maria Helena GaldinoRamos,Livia de SouzaBarbedo,Leonardo SilvaChaves,Alessandra Leal da SilvaMuramoto,Ilda AkemiSantos,André Luis Souza dosAlmeida-Paes,RodrigoZancopé-Oliveira,Rosely Mariaeng2020-04-25T17:52:17Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:20:53.791Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
title |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
spellingShingle |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes Figueiredo-Carvalho,Maria Helena Galdino Candida nivariensis Brazil antifungal resistance hydrolytic enzymes adherence biofilm |
title_short |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
title_full |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
title_fullStr |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
title_full_unstemmed |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
title_sort |
First description of Candida nivariensis in Brazil: antifungal susceptibility profile and potential virulence attributes |
author |
Figueiredo-Carvalho,Maria Helena Galdino |
author_facet |
Figueiredo-Carvalho,Maria Helena Galdino Ramos,Livia de Souza Barbedo,Leonardo Silva Chaves,Alessandra Leal da Silva Muramoto,Ilda Akemi Santos,André Luis Souza dos Almeida-Paes,Rodrigo Zancopé-Oliveira,Rosely Maria |
author_role |
author |
author2 |
Ramos,Livia de Souza Barbedo,Leonardo Silva Chaves,Alessandra Leal da Silva Muramoto,Ilda Akemi Santos,André Luis Souza dos Almeida-Paes,Rodrigo Zancopé-Oliveira,Rosely Maria |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Figueiredo-Carvalho,Maria Helena Galdino Ramos,Livia de Souza Barbedo,Leonardo Silva Chaves,Alessandra Leal da Silva Muramoto,Ilda Akemi Santos,André Luis Souza dos Almeida-Paes,Rodrigo Zancopé-Oliveira,Rosely Maria |
dc.subject.por.fl_str_mv |
Candida nivariensis Brazil antifungal resistance hydrolytic enzymes adherence biofilm |
topic |
Candida nivariensis Brazil antifungal resistance hydrolytic enzymes adherence biofilm |
dc.description.none.fl_txt_mv |
This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management. |
description |
This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilm-growing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000100051 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0074-02760150376 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.111 n.1 2016 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
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1669937720263180288 |