Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection

Detalhes bibliográficos
Autor(a) principal: Eickhoff,Christopher S
Data de Publicação: 2010
Outros Autores: Giddings,Olivia K, Yoshida,Nobuko, Hoft,Daniel F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500015
Resumo: The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
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spelling Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infectionTrypanosoma cruzimucosal immunityvaccinesThe potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.Instituto Oswaldo Cruz, Ministério da Saúde2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500015Memórias do Instituto Oswaldo Cruz v.105 n.5 2010reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762010000500015info:eu-repo/semantics/openAccessEickhoff,Christopher SGiddings,Olivia KYoshida,NobukoHoft,Daniel Feng2020-04-25T17:50:50Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:11.75Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
spellingShingle Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
Eickhoff,Christopher S
Trypanosoma cruzi
mucosal immunity
vaccines
title_short Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_full Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_fullStr Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_full_unstemmed Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_sort Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
author Eickhoff,Christopher S
author_facet Eickhoff,Christopher S
Giddings,Olivia K
Yoshida,Nobuko
Hoft,Daniel F
author_role author
author2 Giddings,Olivia K
Yoshida,Nobuko
Hoft,Daniel F
author2_role author
author
author
dc.contributor.author.fl_str_mv Eickhoff,Christopher S
Giddings,Olivia K
Yoshida,Nobuko
Hoft,Daniel F
dc.subject.por.fl_str_mv Trypanosoma cruzi
mucosal immunity
vaccines
topic Trypanosoma cruzi
mucosal immunity
vaccines
dc.description.none.fl_txt_mv The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
description The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500015
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000500015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762010000500015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.105 n.5 2010
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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