Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses
Main Author: | |
---|---|
Publication Date: | 1994 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Memórias do Instituto Oswaldo Cruz |
Download full: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200011 |
Summary: | Studies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodologyin search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodologyfor detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7 (per cent) for influenzavirus and 60.7 (per cent) for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4 per cent) and NDV (7.2 per cent) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection. |
id |
FIOCRUZ-4_c4dbc21ea46cab8763aa01d10584385f |
---|---|
oai_identifier_str |
oai:scielo:S0074-02761994000200011 |
network_acronym_str |
FIOCRUZ-4 |
network_name_str |
Memórias do Instituto Oswaldo Cruz |
spelling |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruseshemagglutination assaylection neuraminidase assayinfluenza virusesparainfluenza virusesStudies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodologyin search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodologyfor detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7 (per cent) for influenzavirus and 60.7 (per cent) for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4 per cent) and NDV (7.2 per cent) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection.Instituto Oswaldo Cruz, Ministério da Saúde1994-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200011Memórias do Instituto Oswaldo Cruz v.89 n.2 1994reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761994000200011info:eu-repo/semantics/openAccessCouceiro,J. N. S. S.Couceiro,E. S. S.Pinto,A. M. V.Machado,R. D.Cabral,M. C.eng2020-04-25T17:47:15Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:05:56.533Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
title |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
spellingShingle |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses Couceiro,J. N. S. S. hemagglutination assay lection neuraminidase assay influenza viruses parainfluenza viruses |
title_short |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
title_full |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
title_fullStr |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
title_full_unstemmed |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
title_sort |
Comparative evaluation of a simple and sensitive assay for detection of orthomyxo and paramyxoviruses |
author |
Couceiro,J. N. S. S. |
author_facet |
Couceiro,J. N. S. S. Couceiro,E. S. S. Pinto,A. M. V. Machado,R. D. Cabral,M. C. |
author_role |
author |
author2 |
Couceiro,E. S. S. Pinto,A. M. V. Machado,R. D. Cabral,M. C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Couceiro,J. N. S. S. Couceiro,E. S. S. Pinto,A. M. V. Machado,R. D. Cabral,M. C. |
dc.subject.por.fl_str_mv |
hemagglutination assay lection neuraminidase assay influenza viruses parainfluenza viruses |
topic |
hemagglutination assay lection neuraminidase assay influenza viruses parainfluenza viruses |
dc.description.none.fl_txt_mv |
Studies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodologyin search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodologyfor detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7 (per cent) for influenzavirus and 60.7 (per cent) for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4 per cent) and NDV (7.2 per cent) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection. |
description |
Studies were done to evaluate comparatively the traditional HA assay and a more recently introduced lectin-neuraminidase (LN) methodologyin search of a simple and sensitive assay for virus detection during laboratorial diagnosis. The results proved the value of LN assay as a sensitive methodologyfor detection of virus particles, presenting results at least equal to those obtained by HA (hemagglutination) assay, with significant values of accumulated frequencies for LN/HA factors (ratios between LN and HA titers) higher than two. The accumulated values of frequencies for LN/HA factors as high as four were very significant, 72.7 (per cent) for influenzavirus and 60.7 (per cent) for Newcastle disease virus (NDV), moreover accumulated frequencies for LN/HA factors even as high as 32 were due to influenzavirus (45.4 per cent) and NDV (7.2 per cent) samples. After the storage period, most of those concentraded samples that even did not present HA titers could be detected through LN assay, demonstrating a lower threshold for virus detection. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200011 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000200011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761994000200011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.89 n.2 1994 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937662344036352 |