Detalhes bibliográficos
Autor(a) principal: |
Atta,AM |
Data de Publicação: |
2002 |
Outros Autores: |
Sousa-Atta,MLB,
D'Oliveira,A,
Almeida,RP,
Araújo,MI,
Carvalho,EM |
Tipo de documento: |
Artigo
|
Idioma: |
eng |
Título da fonte: |
Memórias do Instituto Oswaldo Cruz |
Texto Completo: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000100017
|
Resumo: |
Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera. |