Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells

Detalhes bibliográficos
Autor(a) principal: Castro,Solange L. de
Data de Publicação: 1992
Outros Autores: Soeiro,Maria Nazaré C., Meirelles,Maria de Nazareth Leal de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007
Resumo: Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.
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spelling Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cellsTrypanosoma cruziphenothiazinescalcium antagonistsparasite-host cell interactionsPhenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.Instituto Oswaldo Cruz, Ministério da Saúde1992-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007Memórias do Instituto Oswaldo Cruz v.87 n.2 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000200007info:eu-repo/semantics/openAccessCastro,Solange L. deSoeiro,Maria Nazaré C.Meirelles,Maria de Nazareth Leal deeng2020-04-25T17:46:43Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:35.546Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
title Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
spellingShingle Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
Castro,Solange L. de
Trypanosoma cruzi
phenothiazines
calcium antagonists
parasite-host cell interactions
title_short Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
title_full Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
title_fullStr Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
title_full_unstemmed Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
title_sort Trypanosoma cruzi: effect of phenothiazines on the parasite and its interaction with host cells
author Castro,Solange L. de
author_facet Castro,Solange L. de
Soeiro,Maria Nazaré C.
Meirelles,Maria de Nazareth Leal de
author_role author
author2 Soeiro,Maria Nazaré C.
Meirelles,Maria de Nazareth Leal de
author2_role author
author
dc.contributor.author.fl_str_mv Castro,Solange L. de
Soeiro,Maria Nazaré C.
Meirelles,Maria de Nazareth Leal de
dc.subject.por.fl_str_mv Trypanosoma cruzi
phenothiazines
calcium antagonists
parasite-host cell interactions
topic Trypanosoma cruzi
phenothiazines
calcium antagonists
parasite-host cell interactions
dc.description.none.fl_txt_mv Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.
description Phenothiazines were observed to have a direct effect on Trypanosoma cruzi and on its in vitro interaction with host cells. They caused lysis of trypomastigotes (50 uM/24 h) and,to a lesser extent, epimastigote proliferation. Treatment of infected peritoneal macrophages with 12.5 uM chlorpromazine or triflupromazine inhibited the infection; this effect was found to be partially reversible if the drugs were removed after 24 h of treatment. At 60 uM, the drugs caused damage to amastigotes interiorized in heart muscle cells. However, the narrow margin of toxity between anti-trypanossomal activity and damage to host cells mitigates against in vivo investigation at the present time. Possible hypothesis for the mechanism of action of phenothiazines are discussed.
publishDate 1992
dc.date.none.fl_str_mv 1992-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000200007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 n.2 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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