Investigation of polymorphisms in the genome of Dengue virus

• Autor(a) principal: Araújo, Roberto Fabian Santos de
• Data de Publicação: 2007
• Outros Autores: Carels, Nicolas, de Melo, Paulo Roberto Santana, Frias Suárez, Diego Gervásio
• Tipo de documento: Artigo
• Idioma: por; eng
• Título da fonte: RECIIS (Online)
• Texto Completo: https://www.reciis.icict.fiocruz.br/index.php/reciis/article/view/927
• topic: Dengue; NS5; polymerase; virulence; polymorphism; Dengue; NS5; polimerase; virulência; polimorfismo

In the present study, we investigated the nucleotide polymorphisms among the four genotypes of the Dengue virus - DENV-1, DENV-2, DENV-3 and DENV-4 - by qualitative and quantitative characterization. We studied the Nucleotide Substitution Density (NSD) along each genome identifying the regions with higher degree of mutation and/or conservation. Then, we calculated the Average Nucleotide Substitution Density (ANSD) for each serotype. We observed that the ANSD of DENV-2 is larger than for DENV-1 by 44.21%, DENV-3 by 85%, and DENV-4 by 163.31%. In contrast to DENV-2 and DENV-4, DENV-1 and DENV-3 showed a similar mutational behavior. The NS5 domain from DENV-2 that corresponds to the RNA-dependent RNA polymerase also has a higher mutation rate compared to that of the other DENVs. This suggests that the polymorphism and the virulence can be correlated in DENV-2, which could contribute to the understanding of the disease evolution.