Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000600610 |
Resumo: | This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL. |
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Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Modelsdentinhardnessscanning electron microscopyFourier-transformed Raman spectroscopyenergy dispersive x-ray spectroscopyThis study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL.Fundação Odontológica de Ribeirão Preto2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000600610Brazilian Dental Journal v.24 n.6 2013reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302357info:eu-repo/semantics/openAccessPacheco,Larissa FerreiraBanzi,Efani Caroline de FreitasRodrigues,ElianaSoares,Luis Eduardo SilvaPascon,Fernanda MioriCorrer-Sobrinho,LourencoPuppin-Rontani,Regina Mariaeng2014-01-14T00:00:00Zoai:scielo:S0103-64402013000600610Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2014-01-14T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
title |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
spellingShingle |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models Pacheco,Larissa Ferreira dentin hardness scanning electron microscopy Fourier-transformed Raman spectroscopy energy dispersive x-ray spectroscopy |
title_short |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
title_full |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
title_fullStr |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
title_full_unstemmed |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
title_sort |
Molecular and Structural Evaluation of Dentin Caries-Like Lesions Produced by Different Artificial Models |
author |
Pacheco,Larissa Ferreira |
author_facet |
Pacheco,Larissa Ferreira Banzi,Efani Caroline de Freitas Rodrigues,Eliana Soares,Luis Eduardo Silva Pascon,Fernanda Miori Correr-Sobrinho,Lourenco Puppin-Rontani,Regina Maria |
author_role |
author |
author2 |
Banzi,Efani Caroline de Freitas Rodrigues,Eliana Soares,Luis Eduardo Silva Pascon,Fernanda Miori Correr-Sobrinho,Lourenco Puppin-Rontani,Regina Maria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pacheco,Larissa Ferreira Banzi,Efani Caroline de Freitas Rodrigues,Eliana Soares,Luis Eduardo Silva Pascon,Fernanda Miori Correr-Sobrinho,Lourenco Puppin-Rontani,Regina Maria |
dc.subject.por.fl_str_mv |
dentin hardness scanning electron microscopy Fourier-transformed Raman spectroscopy energy dispersive x-ray spectroscopy |
topic |
dentin hardness scanning electron microscopy Fourier-transformed Raman spectroscopy energy dispersive x-ray spectroscopy |
description |
This study evaluated structural and molecular issues of dentin caries-like lesions produced by different artificial models (ACL) compared with natural caries lesions (NCL). One hundred twenty-four sound occlusal dentin blocks and 47 carious blocks were obtained and surface hardness was analyzed (SH1). They were assigned to groups according to ACL: GB: Biological; GC: Chemical; GIS: In situ; GNC: natural caries (control). Blocks from groups 1, 2 and 3 were submitted to caries lesion induction. NCL and ACL blocks were submitted to surface hardness (SH 2), FT-Raman and µEDXRF analysis. All blocks were longitudinally sectioned and one of the halves was submitted to cross-sectional hardness (CSH) and the other to SEM analysis. SH1 and SH2 data were submitted to t test (unpaired and paired, respectively), CSH and SEM data to two-way and one-way ANOVA respectively, and Tukey and t tests, respectively (p<0.05). Data from FT-Raman/µEDXRF were submitted to one-way ANOVA and Dunnett multiple-comparisons test (a=0.05). GB and GNC showed lowest SH2 values that were significantly different from GC and GIS. Regarding CSH, GB and GNC showed no significant difference between them. SEM showed similar caries lesion depth for GB and GNC, being significantly higher than for GC and GIS. µEDXRF showed similar values of calcium and phosphate for GB and GNC; GNC values were significantly different from GIS. No significant difference was found among the groups concerning phosphate, carbonate and CH bonds values. For collagen type I, GC values were significantly different compared to other groups. It may be concluded that caries-like lesions produced by GB were the closest model to NCL. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000600610 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402013000600610 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201302357 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.24 n.6 2013 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204093008576512 |