Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400423 |
Resumo: | Abstract The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test. Tests for enterobacteria producing extended spectrumβ-lactamases (ESBL) were performed using agar diffusion. Descriptive statistics was used for data analysis. Two of the isolated strains were submitted to the susceptibility test in biofilm. Of the collected samples, 8% presented Enterobacteria (mean of 6,141 CFU/mL) and no Pseudomona species was isolated. Positive samples were from NB in accommodation set or in the NB nursery. Enterobacter was the most prevalent genus and some strains were resistant to ampicillin, gentamicin and cephalothin. No ESBL strain was detected. Microorganisms in biofilms were resistant to all antibiotics, with concentrations four times higher than MIC. The presence of enterobacteria in the oral cavity of newborns, especially some strains resistant to normally used antibiotics, warns to the need for care to avoid the early colonization of this niche and the occurrence of a possible hospital infection in this age group. |
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Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral CavitynewbornEnterobacteriaceaePseudomonasmultidrug resistancebiofilm.Abstract The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test. Tests for enterobacteria producing extended spectrumβ-lactamases (ESBL) were performed using agar diffusion. Descriptive statistics was used for data analysis. Two of the isolated strains were submitted to the susceptibility test in biofilm. Of the collected samples, 8% presented Enterobacteria (mean of 6,141 CFU/mL) and no Pseudomona species was isolated. Positive samples were from NB in accommodation set or in the NB nursery. Enterobacter was the most prevalent genus and some strains were resistant to ampicillin, gentamicin and cephalothin. No ESBL strain was detected. Microorganisms in biofilms were resistant to all antibiotics, with concentrations four times higher than MIC. The presence of enterobacteria in the oral cavity of newborns, especially some strains resistant to normally used antibiotics, warns to the need for care to avoid the early colonization of this niche and the occurrence of a possible hospital infection in this age group.Fundação Odontológica de Ribeirão Preto2017-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400423Brazilian Dental Journal v.28 n.4 2017reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201601205info:eu-repo/semantics/openAccessFerreira,Priscila Vitor AlvesAmêndola,IsabelaOliveira,Luciane Dias deSilva,Célia Regina Gonçalves eLeão,Mariella Vieira PereiraSantos,Silvana Soléo Ferreira doseng2017-11-08T00:00:00Zoai:scielo:S0103-64402017000400423Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2017-11-08T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
title |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
spellingShingle |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity Ferreira,Priscila Vitor Alves newborn Enterobacteriaceae Pseudomonas multidrug resistance biofilm. |
title_short |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
title_full |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
title_fullStr |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
title_full_unstemmed |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
title_sort |
Prevalence and Sensitivity of Bacilli and Pseudomonas in the Newborn’s Oral Cavity |
author |
Ferreira,Priscila Vitor Alves |
author_facet |
Ferreira,Priscila Vitor Alves Amêndola,Isabela Oliveira,Luciane Dias de Silva,Célia Regina Gonçalves e Leão,Mariella Vieira Pereira Santos,Silvana Soléo Ferreira dos |
author_role |
author |
author2 |
Amêndola,Isabela Oliveira,Luciane Dias de Silva,Célia Regina Gonçalves e Leão,Mariella Vieira Pereira Santos,Silvana Soléo Ferreira dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Priscila Vitor Alves Amêndola,Isabela Oliveira,Luciane Dias de Silva,Célia Regina Gonçalves e Leão,Mariella Vieira Pereira Santos,Silvana Soléo Ferreira dos |
dc.subject.por.fl_str_mv |
newborn Enterobacteriaceae Pseudomonas multidrug resistance biofilm. |
topic |
newborn Enterobacteriaceae Pseudomonas multidrug resistance biofilm. |
description |
Abstract The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test. Tests for enterobacteria producing extended spectrumβ-lactamases (ESBL) were performed using agar diffusion. Descriptive statistics was used for data analysis. Two of the isolated strains were submitted to the susceptibility test in biofilm. Of the collected samples, 8% presented Enterobacteria (mean of 6,141 CFU/mL) and no Pseudomona species was isolated. Positive samples were from NB in accommodation set or in the NB nursery. Enterobacter was the most prevalent genus and some strains were resistant to ampicillin, gentamicin and cephalothin. No ESBL strain was detected. Microorganisms in biofilms were resistant to all antibiotics, with concentrations four times higher than MIC. The presence of enterobacteria in the oral cavity of newborns, especially some strains resistant to normally used antibiotics, warns to the need for care to avoid the early colonization of this niche and the occurrence of a possible hospital infection in this age group. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400423 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402017000400423 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201601205 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.28 n.4 2017 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204094808981504 |