Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000200109 |
Resumo: | This study used gas chromatography-mass spectrometry (GC-MS) to detect the products formed during the contact of sodium hypochlorite (NaOCl) with bovine pulp and dentin. For analysis of the products formed in the volatile phase, 11 mg of bovine pulp tissue were placed in contact with 0.5%, 2.5% and 5.25% NaOCl until complete tissue dissolution occurred. The solid phase microextraction (SPME) fiber was exposed inside the container through the cover membrane and immediately injected into the GC-MS system. 30 mg of the of dentin were kept in contact with NaOCl, and then the SPME fiber was exposed inside the container through the cover membrane for adsorption of the products and injected into the GC-MS system. The same protocol was used for the aqueous phase. For analysis of the volatile compounds, the final solution was extracted using pure ethyl ether. The suspended particulate phase of the mixture was aspirated, and ether was separated from the aqueous phase of the solution. The ether containing the products that resulted from the chemical interaction of dentin and pulp with the NaOCl was filtered and then injected into the GC-MS system for analysis of the aqueous phase. The aqueous and volatile phases of both dentin and pulp showed the formation of chloroform, hexachloroethane, dichloromethylbenzene and benzaldehyde. In conclusion, organochlorine compounds are generated during the contact of dentin and pulp with NaOCl at concentrations of 0.5%, 2.5% and 5.25%. |
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Brazilian Dental Journal |
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Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulpsodium hypochloriteorganochlorinechloroformhexachloroethanedichloromethylbenzeneThis study used gas chromatography-mass spectrometry (GC-MS) to detect the products formed during the contact of sodium hypochlorite (NaOCl) with bovine pulp and dentin. For analysis of the products formed in the volatile phase, 11 mg of bovine pulp tissue were placed in contact with 0.5%, 2.5% and 5.25% NaOCl until complete tissue dissolution occurred. The solid phase microextraction (SPME) fiber was exposed inside the container through the cover membrane and immediately injected into the GC-MS system. 30 mg of the of dentin were kept in contact with NaOCl, and then the SPME fiber was exposed inside the container through the cover membrane for adsorption of the products and injected into the GC-MS system. The same protocol was used for the aqueous phase. For analysis of the volatile compounds, the final solution was extracted using pure ethyl ether. The suspended particulate phase of the mixture was aspirated, and ether was separated from the aqueous phase of the solution. The ether containing the products that resulted from the chemical interaction of dentin and pulp with the NaOCl was filtered and then injected into the GC-MS system for analysis of the aqueous phase. The aqueous and volatile phases of both dentin and pulp showed the formation of chloroform, hexachloroethane, dichloromethylbenzene and benzaldehyde. In conclusion, organochlorine compounds are generated during the contact of dentin and pulp with NaOCl at concentrations of 0.5%, 2.5% and 5.25%.Fundação Odontológica de Ribeirão Preto2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000200109Brazilian Dental Journal v.25 n.2 2014reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201302404info:eu-repo/semantics/openAccessVarise,Tiago GilioliEstrela,CarlosGuedes,Débora Fernandes CostaSousa-Neto,Manoel DamiãoPécora,Jesus Djalmaeng2014-08-15T00:00:00Zoai:scielo:S0103-64402014000200109Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2014-08-15T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
title |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
spellingShingle |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp Varise,Tiago Gilioli sodium hypochlorite organochlorine chloroform hexachloroethane dichloromethylbenzene |
title_short |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
title_full |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
title_fullStr |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
title_full_unstemmed |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
title_sort |
Detection of Organochlorine Compounds Formed During the Contact of Sodium Hypochlorite with Dentin and Dental Pulp |
author |
Varise,Tiago Gilioli |
author_facet |
Varise,Tiago Gilioli Estrela,Carlos Guedes,Débora Fernandes Costa Sousa-Neto,Manoel Damião Pécora,Jesus Djalma |
author_role |
author |
author2 |
Estrela,Carlos Guedes,Débora Fernandes Costa Sousa-Neto,Manoel Damião Pécora,Jesus Djalma |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Varise,Tiago Gilioli Estrela,Carlos Guedes,Débora Fernandes Costa Sousa-Neto,Manoel Damião Pécora,Jesus Djalma |
dc.subject.por.fl_str_mv |
sodium hypochlorite organochlorine chloroform hexachloroethane dichloromethylbenzene |
topic |
sodium hypochlorite organochlorine chloroform hexachloroethane dichloromethylbenzene |
description |
This study used gas chromatography-mass spectrometry (GC-MS) to detect the products formed during the contact of sodium hypochlorite (NaOCl) with bovine pulp and dentin. For analysis of the products formed in the volatile phase, 11 mg of bovine pulp tissue were placed in contact with 0.5%, 2.5% and 5.25% NaOCl until complete tissue dissolution occurred. The solid phase microextraction (SPME) fiber was exposed inside the container through the cover membrane and immediately injected into the GC-MS system. 30 mg of the of dentin were kept in contact with NaOCl, and then the SPME fiber was exposed inside the container through the cover membrane for adsorption of the products and injected into the GC-MS system. The same protocol was used for the aqueous phase. For analysis of the volatile compounds, the final solution was extracted using pure ethyl ether. The suspended particulate phase of the mixture was aspirated, and ether was separated from the aqueous phase of the solution. The ether containing the products that resulted from the chemical interaction of dentin and pulp with the NaOCl was filtered and then injected into the GC-MS system for analysis of the aqueous phase. The aqueous and volatile phases of both dentin and pulp showed the formation of chloroform, hexachloroethane, dichloromethylbenzene and benzaldehyde. In conclusion, organochlorine compounds are generated during the contact of dentin and pulp with NaOCl at concentrations of 0.5%, 2.5% and 5.25%. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000200109 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402014000200109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201302404 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.25 n.2 2014 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204093059956736 |