Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600536 |
Resumo: | Abstract: The purpose of this study was to evaluate the pH, solubility and antimicrobial action of Calcium Hydroxide Paste (CH), Double Antibiotic Paste (metronidazole+ciprofloxacin-DAP), calcium hydroxide added to DAP (CH/DAP) and Triple Antibiotic Paste (metronidazole + ciprofloxacin+minocycline-TAP). pH (n=10) were measured by pHmeter. Root canals of acrylic teeth (n=10) were filled with the above-mentioned intracanal-dressings, immersed in ultrapure water, and solubility was measured by the difference between the initial and final volume (7,15 and 30 days) by using micro-computed tomography. Enterococcus faecalis biofilm was induced on bovine dentin disc surfaces (n=20), and treated with the pastes for 7 days. Percentage bacterial viability was verified by confocal microscope, with LIVE/DEAD dye. CH and CH/DAP presented the highest pH values. Regarding solubility, after 7 days, antibiotic groups presented significant volume loss. CH and CH/DAP showed no statistical difference compared with the Control in antimicrobial action against E. faecalis biofilm. However, TAP and DAP presented a significant percentage reduction in bacterial population. Due to high solubility of the pastes, renewing antibiotic dressings every 7 days, or using the medications for this period in regeneration protocols is recommended. DAP is indicated for killing E. faecalis in biofilm because it has antimicrobial action similar to TAP. Adding Calcium Hydroxide to DAP significantly decreased its antimicrobial action. In spite of its the low solubility and high pH values, the CH paste showed a low level of antimicrobial action against E. faecalis in biofilm. |
id |
FUNORP-1_64c5f62fa1ad17f8ea3b776e65cc6de4 |
---|---|
oai_identifier_str |
oai:scielo:S0103-64402019000600536 |
network_acronym_str |
FUNORP-1 |
network_name_str |
Brazilian Dental Journal |
repository_id_str |
|
spelling |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodonticscalcium hydroxideantibiotic pastesEnterococcus faecalissolubilityhydroxyl ionsAbstract: The purpose of this study was to evaluate the pH, solubility and antimicrobial action of Calcium Hydroxide Paste (CH), Double Antibiotic Paste (metronidazole+ciprofloxacin-DAP), calcium hydroxide added to DAP (CH/DAP) and Triple Antibiotic Paste (metronidazole + ciprofloxacin+minocycline-TAP). pH (n=10) were measured by pHmeter. Root canals of acrylic teeth (n=10) were filled with the above-mentioned intracanal-dressings, immersed in ultrapure water, and solubility was measured by the difference between the initial and final volume (7,15 and 30 days) by using micro-computed tomography. Enterococcus faecalis biofilm was induced on bovine dentin disc surfaces (n=20), and treated with the pastes for 7 days. Percentage bacterial viability was verified by confocal microscope, with LIVE/DEAD dye. CH and CH/DAP presented the highest pH values. Regarding solubility, after 7 days, antibiotic groups presented significant volume loss. CH and CH/DAP showed no statistical difference compared with the Control in antimicrobial action against E. faecalis biofilm. However, TAP and DAP presented a significant percentage reduction in bacterial population. Due to high solubility of the pastes, renewing antibiotic dressings every 7 days, or using the medications for this period in regeneration protocols is recommended. DAP is indicated for killing E. faecalis in biofilm because it has antimicrobial action similar to TAP. Adding Calcium Hydroxide to DAP significantly decreased its antimicrobial action. In spite of its the low solubility and high pH values, the CH paste showed a low level of antimicrobial action against E. faecalis in biofilm.Fundação Odontológica de Ribeirão Preto2019-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600536Brazilian Dental Journal v.30 n.6 2019reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201902613info:eu-repo/semantics/openAccessZancan,Rafaela FernandesCavenago,Bruno CavaliniOda,Denise FerracioliBramante,Clovis MonteiroAndrade,Flaviana Bombarda deDuarte,Marco Antonio Hungaroeng2019-11-25T00:00:00Zoai:scielo:S0103-64402019000600536Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2019-11-25T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
title |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
spellingShingle |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics Zancan,Rafaela Fernandes calcium hydroxide antibiotic pastes Enterococcus faecalis solubility hydroxyl ions |
title_short |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
title_full |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
title_fullStr |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
title_full_unstemmed |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
title_sort |
Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics |
author |
Zancan,Rafaela Fernandes |
author_facet |
Zancan,Rafaela Fernandes Cavenago,Bruno Cavalini Oda,Denise Ferracioli Bramante,Clovis Monteiro Andrade,Flaviana Bombarda de Duarte,Marco Antonio Hungaro |
author_role |
author |
author2 |
Cavenago,Bruno Cavalini Oda,Denise Ferracioli Bramante,Clovis Monteiro Andrade,Flaviana Bombarda de Duarte,Marco Antonio Hungaro |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zancan,Rafaela Fernandes Cavenago,Bruno Cavalini Oda,Denise Ferracioli Bramante,Clovis Monteiro Andrade,Flaviana Bombarda de Duarte,Marco Antonio Hungaro |
dc.subject.por.fl_str_mv |
calcium hydroxide antibiotic pastes Enterococcus faecalis solubility hydroxyl ions |
topic |
calcium hydroxide antibiotic pastes Enterococcus faecalis solubility hydroxyl ions |
description |
Abstract: The purpose of this study was to evaluate the pH, solubility and antimicrobial action of Calcium Hydroxide Paste (CH), Double Antibiotic Paste (metronidazole+ciprofloxacin-DAP), calcium hydroxide added to DAP (CH/DAP) and Triple Antibiotic Paste (metronidazole + ciprofloxacin+minocycline-TAP). pH (n=10) were measured by pHmeter. Root canals of acrylic teeth (n=10) were filled with the above-mentioned intracanal-dressings, immersed in ultrapure water, and solubility was measured by the difference between the initial and final volume (7,15 and 30 days) by using micro-computed tomography. Enterococcus faecalis biofilm was induced on bovine dentin disc surfaces (n=20), and treated with the pastes for 7 days. Percentage bacterial viability was verified by confocal microscope, with LIVE/DEAD dye. CH and CH/DAP presented the highest pH values. Regarding solubility, after 7 days, antibiotic groups presented significant volume loss. CH and CH/DAP showed no statistical difference compared with the Control in antimicrobial action against E. faecalis biofilm. However, TAP and DAP presented a significant percentage reduction in bacterial population. Due to high solubility of the pastes, renewing antibiotic dressings every 7 days, or using the medications for this period in regeneration protocols is recommended. DAP is indicated for killing E. faecalis in biofilm because it has antimicrobial action similar to TAP. Adding Calcium Hydroxide to DAP significantly decreased its antimicrobial action. In spite of its the low solubility and high pH values, the CH paste showed a low level of antimicrobial action against E. faecalis in biofilm. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600536 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402019000600536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201902613 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.30 n.6 2019 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204095738019840 |