Comparison between DNA obtained from buccal cells of the upper and lower gutter area

Detalhes bibliográficos
Autor(a) principal: Nedel,Fernanda
Data de Publicação: 2009
Outros Autores: Conde,Marcus Cristian Muniz, Oliveira,Isabel Oliveira de, Tarquinio,Sandra Beatriz Chaves, Demarco,Flávio Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402009000400002
Resumo: This study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions. The DNA obtained was qualitatively and quantitatively evaluated by 2 calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8% agarose gel electrophoresis). Data was statistically analyzed by one-way ANOVA (?=0.05). Means and standard derivation (SD) for total DNA yield from the upper and lower gutter area were 12.2 ?g (12.0) and 9.4 ?g (8.5), respectively (p=0.821). There was higher (p<0.05) DNA purity for the upper gutter (1.79; 0.05) when compared to lower gutter area (1.66; 0.10). Regarding to the DNA quality, no differences were observed between the 2 location sites, but all samples showed similar degree of degradation. In conclusion, it would be recommendable that buccal cells for DNA extraction be collected from the upper gutter area in the attempt to increase DNA purity.
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spelling Comparison between DNA obtained from buccal cells of the upper and lower gutter areaDNAmouth mucosaepithelial cellsbuccal cellscytobrushThis study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions. The DNA obtained was qualitatively and quantitatively evaluated by 2 calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8% agarose gel electrophoresis). Data was statistically analyzed by one-way ANOVA (?=0.05). Means and standard derivation (SD) for total DNA yield from the upper and lower gutter area were 12.2 ?g (12.0) and 9.4 ?g (8.5), respectively (p=0.821). There was higher (p<0.05) DNA purity for the upper gutter (1.79; 0.05) when compared to lower gutter area (1.66; 0.10). Regarding to the DNA quality, no differences were observed between the 2 location sites, but all samples showed similar degree of degradation. In conclusion, it would be recommendable that buccal cells for DNA extraction be collected from the upper gutter area in the attempt to increase DNA purity.Fundação Odontológica de Ribeirão Preto2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402009000400002Brazilian Dental Journal v.20 n.4 2009reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402009000400002info:eu-repo/semantics/openAccessNedel,FernandaConde,Marcus Cristian MunizOliveira,Isabel Oliveira deTarquinio,Sandra Beatriz ChavesDemarco,Flávio Fernandoeng2009-12-17T00:00:00Zoai:scielo:S0103-64402009000400002Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2009-12-17T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Comparison between DNA obtained from buccal cells of the upper and lower gutter area
title Comparison between DNA obtained from buccal cells of the upper and lower gutter area
spellingShingle Comparison between DNA obtained from buccal cells of the upper and lower gutter area
Nedel,Fernanda
DNA
mouth mucosa
epithelial cells
buccal cells
cytobrush
title_short Comparison between DNA obtained from buccal cells of the upper and lower gutter area
title_full Comparison between DNA obtained from buccal cells of the upper and lower gutter area
title_fullStr Comparison between DNA obtained from buccal cells of the upper and lower gutter area
title_full_unstemmed Comparison between DNA obtained from buccal cells of the upper and lower gutter area
title_sort Comparison between DNA obtained from buccal cells of the upper and lower gutter area
author Nedel,Fernanda
author_facet Nedel,Fernanda
Conde,Marcus Cristian Muniz
Oliveira,Isabel Oliveira de
Tarquinio,Sandra Beatriz Chaves
Demarco,Flávio Fernando
author_role author
author2 Conde,Marcus Cristian Muniz
Oliveira,Isabel Oliveira de
Tarquinio,Sandra Beatriz Chaves
Demarco,Flávio Fernando
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nedel,Fernanda
Conde,Marcus Cristian Muniz
Oliveira,Isabel Oliveira de
Tarquinio,Sandra Beatriz Chaves
Demarco,Flávio Fernando
dc.subject.por.fl_str_mv DNA
mouth mucosa
epithelial cells
buccal cells
cytobrush
topic DNA
mouth mucosa
epithelial cells
buccal cells
cytobrush
description This study compared quantitatively and qualitatively the DNA extracted from buccal cells collected from the upper or lower gutter areas. Buccal cells were collected from the upper (n=15) and lower gutter (n=15) region from 15 volunteers using a special cytobrush (Gentra), totaling 2 collections from each individual. DNA was extracted from the samples according to the manufacturer's instructions. The DNA obtained was qualitatively and quantitatively evaluated by 2 calibrated blind examiners using spectrophotometry and analysis of DNA bands (0.8% agarose gel electrophoresis). Data was statistically analyzed by one-way ANOVA (?=0.05). Means and standard derivation (SD) for total DNA yield from the upper and lower gutter area were 12.2 ?g (12.0) and 9.4 ?g (8.5), respectively (p=0.821). There was higher (p<0.05) DNA purity for the upper gutter (1.79; 0.05) when compared to lower gutter area (1.66; 0.10). Regarding to the DNA quality, no differences were observed between the 2 location sites, but all samples showed similar degree of degradation. In conclusion, it would be recommendable that buccal cells for DNA extraction be collected from the upper gutter area in the attempt to increase DNA purity.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402009000400002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402009000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402009000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.20 n.4 2009
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
_version_ 1754204090998456320

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