Subcutaneous tissue reaction and gene expression of inflammatory markers after Biodentine and MTA implantation

• Autor(a) principal: Silva,Raquel Assed Bezerra
• Data de Publicação: 2022
• Outros Autores: Gaton-Hernandez,Patrícia, Pucinelli,Carolina Maschietto, Silva,Francisco Wanderley Garcia de Paula e, Lucisano,Marília Pacífico, Consolaro,Alberto, Sá,Rafaela Cardoso de, Araujo,Lisa Danielly Curcino, Sousa-Neto,Manoel Damião, Silva,Léa Assed Bezerra
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Dental Journal
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000100041
• topic: Pro-Root MTA; Biodentine; Immune-inflammatory response; Subcutaneous connective tissue; Cytokines

Abstract The aim of this study was to evaluate the subcutaneous connective tissue response of isogenic mice exposed to tricalcium silicate (Biodentine) and aggregated mineral trioxide (ProRoot MTA). A total of 120 mice were divided into 4 groups in 3 different experimental periods (7, 21 and 63 days): Biodentine; Pro-Root MTA; zinc oxide-eugenol and; Negative control - Sham. After the experimental periods microscopic descriptive, semi-quantitative and quantitative analysis of the inflammatory process were analyzed on H&E sections and evaluation of the gene expression of Il10, Infg, Il6, Il1r1 and Tnf (qRT-PCR) were performed. The data obtained were analyzed using the chi-square test and two-way analysis of variance (ANOVA) followed by the Bonferroni post-test (5% significance level). Results: In the microscopic analysis, a slight inflammatory infiltrate was observed, with a predominance of sparse macrophages and polymorphonuclear cells, slight tissue fibrosis, regular fibrous capsule and with dystrophic calcifications, in all groups that received the materials (Biodentine and Pro-Root MTA). In parallel, all materials modulated the gene expression of the different cytokines and receptors evaluated. Conclusion: Pro-Root MTA and Biodentine showed a tissue compatibility, mediated inflammation, with increased fibrous tissue and production of pro- and anti-inflammatory cytokines.