Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319 |
Resumo: | Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions. |
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Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Studyproteomic analysisdental pulppulpitisdental pulp necrosisendodonticsAbstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions.Fundação Odontológica de Ribeirão Preto2020-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319Brazilian Dental Journal v.31 n.3 2020reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202003167info:eu-repo/semantics/openAccessLoureiro,CarolineBuzalaf,Marília Afonso RabeloPessan,Juliano PelimMoraes,Felipe Ricardo Nunes dePelá,Vinícius TaioquiVentura,Talita Mendes OliveiraJacinto,Rogério de Castilhoeng2020-07-09T00:00:00Zoai:scielo:S0103-64402020000300319Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2020-07-09T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
spellingShingle |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study Loureiro,Caroline proteomic analysis dental pulp pulpitis dental pulp necrosis endodontics |
title_short |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_full |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_fullStr |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_full_unstemmed |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
title_sort |
Comparative Analysis of the Proteomic Profile of the Dental Pulp in Different Conditions. A Pilot Study |
author |
Loureiro,Caroline |
author_facet |
Loureiro,Caroline Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho |
author_role |
author |
author2 |
Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Loureiro,Caroline Buzalaf,Marília Afonso Rabelo Pessan,Juliano Pelim Moraes,Felipe Ricardo Nunes de Pelá,Vinícius Taioqui Ventura,Talita Mendes Oliveira Jacinto,Rogério de Castilho |
dc.subject.por.fl_str_mv |
proteomic analysis dental pulp pulpitis dental pulp necrosis endodontics |
topic |
proteomic analysis dental pulp pulpitis dental pulp necrosis endodontics |
description |
Abstract This study aimed to quantitatively compare the difference in protein expression in the progression of pulp pathogenesis, as well as to describe the biological functions of proteins identified in pulp tissue. Samples were obtained from six patients treated at the Araçatuba School of Dentistry and were divided into three groups: normal pulp - from teeth extracted for orthodontic indication; inflamed pulp and necrotic pulp - from patients diagnosed with irreversible pulpitis and chronic apical periodontitis, respectively. After previous proteomic preparation, dental pulp samples were processed for label-free quantitative proteomic analysis in a nanoACQUITY UPLC-Xevo QTof MS system. The difference in expression between the groups was calculated using the Protein Lynx Global Service software using the Monte Carlo algorithm. A total of 465 human proteins were identified in all groups. The most expressed proteins in the inflamed pulp group in relation to the normal pulp group were hemoglobin, peroxiredoxins and immunoglobulins, whereas the less expressed were the tubulins. Expression levels of albumins, immunoglobulins and alpha-2-macroglobulin were higher in the necrotic pulp group than in the inflamed pulp group. As for the qualitative analysis, the most prevalent protein functions in the normal pulp group were metabolic and energetic pathways; in the inflamed pulp group: cellular communication and signal transduction; and regulation and repair of DNA/RNA, while in the necrotic pulp group proteins were associated with the immune response. Thus, proteomic analysis showed quantitative and qualitative differences in protein expression in different types of pulp conditions. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402020000300319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202003167 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.31 n.3 2020 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204095839731712 |