Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

Detalhes bibliográficos
Autor(a) principal: Costa,Marília Teixeira
Data de Publicação: 2012
Outros Autores: Dorta,Miriam Leandro, Ribeiro-Dias,Fátima, Pimenta,Fabiana Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Journal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500014
Resumo: This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.
id FUNORP-1_f27e735adb164c089179f9ff94f7d83d
oai_identifier_str oai:scielo:S0103-64402012000500014
network_acronym_str FUNORP-1
network_name_str Brazilian Dental Journal
repository_id_str
spelling Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutansblack tooth staindental cariesmutans streptococciblack-pigmented bacteriaPCRThis study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.Fundação Odontológica de Ribeirão Preto2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500014Brazilian Dental Journal v.23 n.5 2012reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/S0103-64402012000500014info:eu-repo/semantics/openAccessCosta,Marília TeixeiraDorta,Miriam LeandroRibeiro-Dias,FátimaPimenta,Fabiana Cristinaeng2013-01-08T00:00:00Zoai:scielo:S0103-64402012000500014Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2013-01-08T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
title Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
spellingShingle Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
Costa,Marília Teixeira
black tooth stain
dental caries
mutans streptococci
black-pigmented bacteria
PCR
title_short Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
title_full Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
title_fullStr Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
title_full_unstemmed Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
title_sort Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans
author Costa,Marília Teixeira
author_facet Costa,Marília Teixeira
Dorta,Miriam Leandro
Ribeiro-Dias,Fátima
Pimenta,Fabiana Cristina
author_role author
author2 Dorta,Miriam Leandro
Ribeiro-Dias,Fátima
Pimenta,Fabiana Cristina
author2_role author
author
author
dc.contributor.author.fl_str_mv Costa,Marília Teixeira
Dorta,Miriam Leandro
Ribeiro-Dias,Fátima
Pimenta,Fabiana Cristina
dc.subject.por.fl_str_mv black tooth stain
dental caries
mutans streptococci
black-pigmented bacteria
PCR
topic black tooth stain
dental caries
mutans streptococci
black-pigmented bacteria
PCR
description This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402012000500014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-64402012000500014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.23 n.5 2012
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
_version_ 1754204092260941824

Registros relacionados