Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Journal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000600602 |
Resumo: | Mucograft(r) is a resorbing porcine matrix composed of type I and type III collagen, used for soft tissue augmentation in guided tissue bony regeneration procedures. This in vitro study aimed to evaluate the biological behavior of Mucograft(r) in human gingival fibroblasts, as well as the ability of the matrix to induce production of extracellular matrix. Six resorbing Mucograft(r) matrices (MCG) were cut into 3 x 2 mm rectangles and 5 x 5 mm squares and were placed in 96- and 24-well plates, respectively. The control group (CTRL) consisted of cells plated on polystyrene without the MCG. After one, two, three and seven days, cell proliferation and viability were assessed using the Trypan exclusion method and MTT test, respectively. Type III collagen (COL 3A1) and vimentin (VIM) expression were also evaluated at 10 and 14 days, using Western blotting. Statistical analysis, using ANOVA with post hoc Bonferroni test, revealed that human gingival fibroblasts from MCG showed similar results (p>0.05) for proliferation and viability as the cells cultured on CTRL. After 14 days, a significant decrease in COL 3A1 expression (p<0.05) was observed when cultured with the MCG. VIM expression showed no significant difference at any time period (p>0.05). Although no increase in extracellular matrix secretion was observed in this in vitro study, Mucograft(r) presented cellular compatibility, being an option for a scaffold whenever it is required. |
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Brazilian Dental Journal |
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Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Studygingival tissue graftMucograft(r) collagen matrixcell cultureguided tissue regenerationMucograft(r) is a resorbing porcine matrix composed of type I and type III collagen, used for soft tissue augmentation in guided tissue bony regeneration procedures. This in vitro study aimed to evaluate the biological behavior of Mucograft(r) in human gingival fibroblasts, as well as the ability of the matrix to induce production of extracellular matrix. Six resorbing Mucograft(r) matrices (MCG) were cut into 3 x 2 mm rectangles and 5 x 5 mm squares and were placed in 96- and 24-well plates, respectively. The control group (CTRL) consisted of cells plated on polystyrene without the MCG. After one, two, three and seven days, cell proliferation and viability were assessed using the Trypan exclusion method and MTT test, respectively. Type III collagen (COL 3A1) and vimentin (VIM) expression were also evaluated at 10 and 14 days, using Western blotting. Statistical analysis, using ANOVA with post hoc Bonferroni test, revealed that human gingival fibroblasts from MCG showed similar results (p>0.05) for proliferation and viability as the cells cultured on CTRL. After 14 days, a significant decrease in COL 3A1 expression (p<0.05) was observed when cultured with the MCG. VIM expression showed no significant difference at any time period (p>0.05). Although no increase in extracellular matrix secretion was observed in this in vitro study, Mucograft(r) presented cellular compatibility, being an option for a scaffold whenever it is required.Fundação Odontológica de Ribeirão Preto2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000600602Brazilian Dental Journal v.26 n.6 2015reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440201300238info:eu-repo/semantics/openAccessLima,Rafaela S. R. ePeruzzo,Daiane C.Napimoga,Marcelo H.Saba-Chujfi,EduardoSantos-Pereira,Silvio Antonio dosMartinez,Elizabeth F.eng2015-12-22T00:00:00Zoai:scielo:S0103-64402015000600602Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2015-12-22T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
title |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
spellingShingle |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study Lima,Rafaela S. R. e gingival tissue graft Mucograft(r) collagen matrix cell culture guided tissue regeneration |
title_short |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
title_full |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
title_fullStr |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
title_full_unstemmed |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
title_sort |
Evaluation of the Biological Behavior of Mucograft® in Human Gingival Fibroblasts: An In Vitro Study |
author |
Lima,Rafaela S. R. e |
author_facet |
Lima,Rafaela S. R. e Peruzzo,Daiane C. Napimoga,Marcelo H. Saba-Chujfi,Eduardo Santos-Pereira,Silvio Antonio dos Martinez,Elizabeth F. |
author_role |
author |
author2 |
Peruzzo,Daiane C. Napimoga,Marcelo H. Saba-Chujfi,Eduardo Santos-Pereira,Silvio Antonio dos Martinez,Elizabeth F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lima,Rafaela S. R. e Peruzzo,Daiane C. Napimoga,Marcelo H. Saba-Chujfi,Eduardo Santos-Pereira,Silvio Antonio dos Martinez,Elizabeth F. |
dc.subject.por.fl_str_mv |
gingival tissue graft Mucograft(r) collagen matrix cell culture guided tissue regeneration |
topic |
gingival tissue graft Mucograft(r) collagen matrix cell culture guided tissue regeneration |
description |
Mucograft(r) is a resorbing porcine matrix composed of type I and type III collagen, used for soft tissue augmentation in guided tissue bony regeneration procedures. This in vitro study aimed to evaluate the biological behavior of Mucograft(r) in human gingival fibroblasts, as well as the ability of the matrix to induce production of extracellular matrix. Six resorbing Mucograft(r) matrices (MCG) were cut into 3 x 2 mm rectangles and 5 x 5 mm squares and were placed in 96- and 24-well plates, respectively. The control group (CTRL) consisted of cells plated on polystyrene without the MCG. After one, two, three and seven days, cell proliferation and viability were assessed using the Trypan exclusion method and MTT test, respectively. Type III collagen (COL 3A1) and vimentin (VIM) expression were also evaluated at 10 and 14 days, using Western blotting. Statistical analysis, using ANOVA with post hoc Bonferroni test, revealed that human gingival fibroblasts from MCG showed similar results (p>0.05) for proliferation and viability as the cells cultured on CTRL. After 14 days, a significant decrease in COL 3A1 expression (p<0.05) was observed when cultured with the MCG. VIM expression showed no significant difference at any time period (p>0.05). Although no increase in extracellular matrix secretion was observed in this in vitro study, Mucograft(r) presented cellular compatibility, being an option for a scaffold whenever it is required. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000600602 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402015000600602 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440201300238 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.26 n.6 2015 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
_version_ |
1754204093926080512 |