Lactose conversion and the synthesis of galactooligosaccharides in a simultaneous lagged bioprocess using β-galactosidases and probiotic microorganisms

• Autor(a) principal: Martins, André Rosa
• Data de Publicação: 2011
• Outros Autores: Manera, Ana Paula, Monteiro, Ricardo Lemos, Burkert, Janaina Fernandes de Medeiros, Burkert, Carlos André Veiga
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/1826
• topic: Enzyme catalysis; Lactose conversion; Lactic fermentation; GOS; Catálise enzimática; Conversão da lactose; Fermentação lática; GOS

Lactose conversion and the production of galactooligosaccharides (GOS) were evaluated in a simultaneous bioprocess using probiotic microorganisms and the addition of β-galactosidases, lagged or otherwise. The fermentation was carried out using normal yogurt cultures, Streptococcus thermophilus and Lactobacillus bulgaricus, associated with two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus. An enzymatic preparation containing β-galactosidases of distinct origins, obtained from Kluyveromyces lactis and Aspergillus niger was used. The simultaneous bioprocess was evaluated, varying the initial lactose and enzyme concentrations and also the time of addition of the enzymes, with a lag in the enzyme application in relation to the beginning of fermentation. The results indicated a positive impact on lactose conversion, reducing the disaccharide concentration by between 97.7 and 99.7%, as compared to 13.8 and 19.8% in fermentations without enzyme addition, obtaining between 2.7 and 4.2 g.L-1 GOS in the lagged processes carried out with the highest initial lactose concentration and the addition of the lowest enzyme concentration.