Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)

Detalhes bibliográficos
Autor(a) principal: Peixoto, Silvio Ricardo Maurano
Data de Publicação: 2002
Outros Autores: Garcia, Nieves Aguado, D'Incao, Fernando, Wasielesky Junior, Wilson Francisco Britto, Cousin, João Carlos Brahm
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/2986
http://dx.doi.org/10.1590/S1519-69842002000500017
Resumo: A preliminary study was done on the age-pigment lipofuscin content in the brains of captive Farfantepenaeus paulensis juveniles (5 months old) and wild adults (estimated age of 12-15 months). Random samples of 6 individuals were obtained from each group (juvenile and adult) for histological analysis. Serial sections (6 mm) of the brains were mounted without staining and observed in an epifluorescent microscope. The fluorescent images of the five most central sections of the olfactory lobe cell mass (OLCM) of each individual were digitized for image analysis. The lipofuscin granule mean diameter was similar in both groups (p > 0.05), however the lipofuscin area fraction (percentage of the OLCM occupied by pigment granules) was significantly higher (p < 0.05) in the adult shrimp. The detection of lipofuscin granules in 5 month old F. paulensis indicates that lipofuscin deposition probably takes place even earlier in the juvenile phase. Our results suggested that the amount of granules in the F. paulensis OLCM is related to age, but further studies are necessary to evaluate the relationship between lipofuscin content and the age of captive F. paulensis.
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spelling Peixoto, Silvio Ricardo MauranoGarcia, Nieves AguadoD'Incao, FernandoWasielesky Junior, Wilson Francisco BrittoCousin, João Carlos Brahm2012-12-11T17:19:50Z2012-12-11T17:19:50Z2002PEIXOTO, Silvio Ricardo Maurano et al. Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda). Brazilian Journal Of Biolgy, Rio de Janeiro, v. 62, n.4B, p. 871-876, 2002. Disponível em: <http://www.scielo.br/pdf/bjb/v62n4b/a17v624b.pdf>. Acesso em 06 dez. 2012.1519-6984http://repositorio.furg.br/handle/1/2986http://dx.doi.org/10.1590/S1519-69842002000500017A preliminary study was done on the age-pigment lipofuscin content in the brains of captive Farfantepenaeus paulensis juveniles (5 months old) and wild adults (estimated age of 12-15 months). Random samples of 6 individuals were obtained from each group (juvenile and adult) for histological analysis. Serial sections (6 mm) of the brains were mounted without staining and observed in an epifluorescent microscope. The fluorescent images of the five most central sections of the olfactory lobe cell mass (OLCM) of each individual were digitized for image analysis. The lipofuscin granule mean diameter was similar in both groups (p > 0.05), however the lipofuscin area fraction (percentage of the OLCM occupied by pigment granules) was significantly higher (p < 0.05) in the adult shrimp. The detection of lipofuscin granules in 5 month old F. paulensis indicates that lipofuscin deposition probably takes place even earlier in the juvenile phase. Our results suggested that the amount of granules in the F. paulensis OLCM is related to age, but further studies are necessary to evaluate the relationship between lipofuscin content and the age of captive F. paulensis.Foi realizada análise preliminar do conteúdo de lipofuscina em cérebros de juvenis de Farfantepenaeus paulensis oriundos de cativeiro (5 meses de idade) e de adultos selvagens (idade estimada de 12-15 meses). Foram coletadas amostras aleatórias de 6 indivíduos de cada grupo (juvenis e adultos) para análises histológicas. Cortes frontais (6 mm) dos cérebros foram montados em lâminas, sem serem corados, e observados em microscópio de epifluorescência. As imagens fluorescentes dos 5 cortes mais centrais da massa de células do lóbulo olfatório (MCLO) de cada indivíduo foram digitalizadas para posterior análise de imagens. O diâmetro médio dos grânulos de lipofuscina foram similares entre os dois grupos (p > 0,05), entretanto, a porcentagem da área de MCLO ocupada por grânulos foi significativamente maior (p < 0,05) nos camarões adultos. A detecção de grânulos de lipofuscina em F. paulensis com 5 meses de idade indica a possibilidade de o início de deposição ocorrer anteriormente em juvenis. Os resultados sugerem que a quantidade de grânulos na MCLO de F. paulensis está relacionada à idade, contudo, futuros estudos são necessários para avaliar a relação entre a quantidade de lipofuscina e a idade de F. paulensis mantidos em cativeiro.engLipofuscinAge-pigmentPenaeidF.PaulensisCipofuscinaPigmentoIdadePeneídeoF. paulensisPreliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)Identificação e quantificação preliminar do pigmento da idade, lipofuscina, em cérebros de farfantepenaeus paulensis (crustacea: decapoda)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/2986/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open accessORIGINALPreliminary.pdfPreliminary.pdfapplication/pdf176178https://repositorio.furg.br/bitstream/1/2986/1/Preliminary.pdfb0e0c9fb028d9756bf7c97adb30b2eebMD51open access1/29862012-12-11 15:19:50.184open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-12-11T17:19:50Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
dc.title.alternative.pt_BR.fl_str_mv Identificação e quantificação preliminar do pigmento da idade, lipofuscina, em cérebros de farfantepenaeus paulensis (crustacea: decapoda)
title Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
spellingShingle Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
Peixoto, Silvio Ricardo Maurano
Lipofuscin
Age-pigment
Penaeid
F.Paulensis
Cipofuscina
Pigmento
Idade
Peneídeo
F. paulensis
title_short Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
title_full Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
title_fullStr Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
title_full_unstemmed Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
title_sort Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda)
author Peixoto, Silvio Ricardo Maurano
author_facet Peixoto, Silvio Ricardo Maurano
Garcia, Nieves Aguado
D'Incao, Fernando
Wasielesky Junior, Wilson Francisco Britto
Cousin, João Carlos Brahm
author_role author
author2 Garcia, Nieves Aguado
D'Incao, Fernando
Wasielesky Junior, Wilson Francisco Britto
Cousin, João Carlos Brahm
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Peixoto, Silvio Ricardo Maurano
Garcia, Nieves Aguado
D'Incao, Fernando
Wasielesky Junior, Wilson Francisco Britto
Cousin, João Carlos Brahm
dc.subject.por.fl_str_mv Lipofuscin
Age-pigment
Penaeid
F.Paulensis
Cipofuscina
Pigmento
Idade
Peneídeo
F. paulensis
topic Lipofuscin
Age-pigment
Penaeid
F.Paulensis
Cipofuscina
Pigmento
Idade
Peneídeo
F. paulensis
description A preliminary study was done on the age-pigment lipofuscin content in the brains of captive Farfantepenaeus paulensis juveniles (5 months old) and wild adults (estimated age of 12-15 months). Random samples of 6 individuals were obtained from each group (juvenile and adult) for histological analysis. Serial sections (6 mm) of the brains were mounted without staining and observed in an epifluorescent microscope. The fluorescent images of the five most central sections of the olfactory lobe cell mass (OLCM) of each individual were digitized for image analysis. The lipofuscin granule mean diameter was similar in both groups (p > 0.05), however the lipofuscin area fraction (percentage of the OLCM occupied by pigment granules) was significantly higher (p < 0.05) in the adult shrimp. The detection of lipofuscin granules in 5 month old F. paulensis indicates that lipofuscin deposition probably takes place even earlier in the juvenile phase. Our results suggested that the amount of granules in the F. paulensis OLCM is related to age, but further studies are necessary to evaluate the relationship between lipofuscin content and the age of captive F. paulensis.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2012-12-11T17:19:50Z
dc.date.available.fl_str_mv 2012-12-11T17:19:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv PEIXOTO, Silvio Ricardo Maurano et al. Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda). Brazilian Journal Of Biolgy, Rio de Janeiro, v. 62, n.4B, p. 871-876, 2002. Disponível em: <http://www.scielo.br/pdf/bjb/v62n4b/a17v624b.pdf>. Acesso em 06 dez. 2012.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/2986
dc.identifier.issn.none.fl_str_mv 1519-6984
dc.identifier.doi.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S1519-69842002000500017
identifier_str_mv PEIXOTO, Silvio Ricardo Maurano et al. Preliminary identification and quantification of the age-pigment lipofuscin in the brain of farfantepenaeus paulensis (crustacea: decapoda). Brazilian Journal Of Biolgy, Rio de Janeiro, v. 62, n.4B, p. 871-876, 2002. Disponível em: <http://www.scielo.br/pdf/bjb/v62n4b/a17v624b.pdf>. Acesso em 06 dez. 2012.
1519-6984
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http://dx.doi.org/10.1590/S1519-69842002000500017
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