Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/1731 |
Resumo: | Recovery and alteration of fish protein from wastes and its use has been regarded as a promising alternative to develop useful products once polymer gels have a high capacity of water uptake. This study aims to produce hydrogel, a super absorbent biopolymer from modified fish protein, in order to evaluate the protein structure. In the modified proteins, analyses of the extent of modification of the lysine residues, electrophoresis, and electrometric titration were performed. In the hydrogels were realized assays of swelling water. The proteins with more modifications were shown as 63.5% and 75.9% of lysine residues, from fish protein isolate obtained with alkaline and acid solubilization, respectively. The modified protein in that same rate presented 332.0 and 311.4 carboxyl groups. Accordingly, the hydrogel produced from alkaline and acid isolates reached a maximum water uptake in 24 h of 79.42 and 103.25 gwater/gdry gel, respectively. |
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Martins, Vilásia GuimarãesCosta, Jorge Alberto VieiraDamodaran, SrinivasanHernandez, Carlos Prentice2012-01-08T00:00:29Z2012-01-08T00:00:29Z2011MARTINS, Vilásia Guimarães et al. Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes. Appl Biochem Biotechnol, v. 165, n. 1, p. 279–289, 2011. Disponível em: <http://www.springerlink.com/content/r51628p074460265/fulltext.pdf> Acesso em: 19 dez. 2011.0273-2289http://repositorio.furg.br/handle/1/173110.1007/s12010-011-9250-yRecovery and alteration of fish protein from wastes and its use has been regarded as a promising alternative to develop useful products once polymer gels have a high capacity of water uptake. This study aims to produce hydrogel, a super absorbent biopolymer from modified fish protein, in order to evaluate the protein structure. In the modified proteins, analyses of the extent of modification of the lysine residues, electrophoresis, and electrometric titration were performed. In the hydrogels were realized assays of swelling water. The proteins with more modifications were shown as 63.5% and 75.9% of lysine residues, from fish protein isolate obtained with alkaline and acid solubilization, respectively. The modified protein in that same rate presented 332.0 and 311.4 carboxyl groups. Accordingly, the hydrogel produced from alkaline and acid isolates reached a maximum water uptake in 24 h of 79.42 and 103.25 gwater/gdry gel, respectively.engFishWasteHydrogelBiopolymerSuper absorbentProtein isolateChemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGLICENSElicense.txtlicense.txttext/plain; charset=utf-81724https://repositorio.furg.br/bitstream/1/1731/2/license.txt5b92b9704b4f13242d70e45ddef35a68MD52open accessORIGINALChemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes.pdfChemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes.pdfapplication/pdf275088https://repositorio.furg.br/bitstream/1/1731/1/Chemical%20modification%20and%20structural%20analysis%20of%20%20protein%20isolates%20to%20produce%20hydrogel%20using%20whitemouth%20croaker%20%28micropogonias%20furnieri%29%20wastes.pdfd3842871c92cfbb3e19be8798c389ad7MD51open access1/17312012-01-07 22:00:29.691open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-01-08T00:00:29Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
title |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
spellingShingle |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes Martins, Vilásia Guimarães Fish Waste Hydrogel Biopolymer Super absorbent Protein isolate |
title_short |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
title_full |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
title_fullStr |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
title_full_unstemmed |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
title_sort |
Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes |
author |
Martins, Vilásia Guimarães |
author_facet |
Martins, Vilásia Guimarães Costa, Jorge Alberto Vieira Damodaran, Srinivasan Hernandez, Carlos Prentice |
author_role |
author |
author2 |
Costa, Jorge Alberto Vieira Damodaran, Srinivasan Hernandez, Carlos Prentice |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, Vilásia Guimarães Costa, Jorge Alberto Vieira Damodaran, Srinivasan Hernandez, Carlos Prentice |
dc.subject.por.fl_str_mv |
Fish Waste Hydrogel Biopolymer Super absorbent Protein isolate |
topic |
Fish Waste Hydrogel Biopolymer Super absorbent Protein isolate |
description |
Recovery and alteration of fish protein from wastes and its use has been regarded as a promising alternative to develop useful products once polymer gels have a high capacity of water uptake. This study aims to produce hydrogel, a super absorbent biopolymer from modified fish protein, in order to evaluate the protein structure. In the modified proteins, analyses of the extent of modification of the lysine residues, electrophoresis, and electrometric titration were performed. In the hydrogels were realized assays of swelling water. The proteins with more modifications were shown as 63.5% and 75.9% of lysine residues, from fish protein isolate obtained with alkaline and acid solubilization, respectively. The modified protein in that same rate presented 332.0 and 311.4 carboxyl groups. Accordingly, the hydrogel produced from alkaline and acid isolates reached a maximum water uptake in 24 h of 79.42 and 103.25 gwater/gdry gel, respectively. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2012-01-08T00:00:29Z |
dc.date.available.fl_str_mv |
2012-01-08T00:00:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MARTINS, Vilásia Guimarães et al. Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes. Appl Biochem Biotechnol, v. 165, n. 1, p. 279–289, 2011. Disponível em: <http://www.springerlink.com/content/r51628p074460265/fulltext.pdf> Acesso em: 19 dez. 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/1731 |
dc.identifier.issn.none.fl_str_mv |
0273-2289 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1007/s12010-011-9250-y |
identifier_str_mv |
MARTINS, Vilásia Guimarães et al. Chemical modification and structural analysis of protein isolates to produce hydrogel using whitemouth croaker (micropogonias furnieri) wastes. Appl Biochem Biotechnol, v. 165, n. 1, p. 279–289, 2011. Disponível em: <http://www.springerlink.com/content/r51628p074460265/fulltext.pdf> Acesso em: 19 dez. 2011. 0273-2289 10.1007/s12010-011-9250-y |
url |
http://repositorio.furg.br/handle/1/1731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
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Universidade Federal do Rio Grande (FURG) |
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FURG |
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FURG |
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Repositório Institucional da FURG (RI FURG) |
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Repositório Institucional da FURG (RI FURG) |
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