Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/2367 http://dx.doi.org/10.1007/s10811-009-9451-8 |
Resumo: | Cyanobacterial blooms and the accompanying production of cyanotoxins are a serious global problem. Toxic blooms of Anabaena species are common in lagoons and reservoirs of southern Brazil. Worldwide, species of the genus Anabaena produce the majority of the known hepatotoxins (microcystins) and neurotoxins [anatoxin-a, anatoxin-a(s), and saxitoxins]. This report links a bloom of Anabaena crassa in the Faxinal Reservoir, the main water supply for the city of Caxias do Sul (400,000 inhabitants) in southern Brazil, to the occurrence of anatoxin-a(s) in the water. During the bloom period, the reservoir was strongly stratified, with higher temperatures and a deep anoxic hypolimnion. Two methods for sample concentration (direct and complete extraction) were tested, and direct extraction of samples proved to be more efficient. Water samples collected during the bloom showed 9% acetylcholinesterase inhibition at 50 mg mL−1, corresponding to 0.61μg of anatoxin-a(s) per gram of lyophilized powder. At these concentrations, symptoms of neurotoxicity and mortality were not observed in tests with Swiss albino mice. Although the concentrations of anatoxin-a(s) in the Faxinal Reservoir were low, these results are important because this is the first record of the toxin for A. crassa. Furthermore, this cyanotoxin is not yet included in Brazilian legislation for drinking-water monitoring, because of the lack of information about toxicity levels and risk calculation for oral doses. The data presented here contribute to the basis for the future inclusion of this toxin in Brazilian legislation for drinking-water quality control, and for the development of analytical methods for this toxin. |
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Becker, VanessaIhara, PriscilaYunes, João SarkisHuszar, Vera Lúcia de Moraes2012-08-15T21:25:59Z2012-08-15T21:25:59Z2010BECKER, Vanessa et al. Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil. Journal of Applied Phycology, v.22, n. 3, p. 235-241, 2009. Disponível em: <http://www.springerlink.com/content/n135720126381k55/fulltext.pdf>. Acesso em: 08 jul. 2012.http://repositorio.furg.br/handle/1/2367http://dx.doi.org/10.1007/s10811-009-9451-8Cyanobacterial blooms and the accompanying production of cyanotoxins are a serious global problem. Toxic blooms of Anabaena species are common in lagoons and reservoirs of southern Brazil. Worldwide, species of the genus Anabaena produce the majority of the known hepatotoxins (microcystins) and neurotoxins [anatoxin-a, anatoxin-a(s), and saxitoxins]. This report links a bloom of Anabaena crassa in the Faxinal Reservoir, the main water supply for the city of Caxias do Sul (400,000 inhabitants) in southern Brazil, to the occurrence of anatoxin-a(s) in the water. During the bloom period, the reservoir was strongly stratified, with higher temperatures and a deep anoxic hypolimnion. Two methods for sample concentration (direct and complete extraction) were tested, and direct extraction of samples proved to be more efficient. Water samples collected during the bloom showed 9% acetylcholinesterase inhibition at 50 mg mL−1, corresponding to 0.61μg of anatoxin-a(s) per gram of lyophilized powder. At these concentrations, symptoms of neurotoxicity and mortality were not observed in tests with Swiss albino mice. Although the concentrations of anatoxin-a(s) in the Faxinal Reservoir were low, these results are important because this is the first record of the toxin for A. crassa. Furthermore, this cyanotoxin is not yet included in Brazilian legislation for drinking-water monitoring, because of the lack of information about toxicity levels and risk calculation for oral doses. The data presented here contribute to the basis for the future inclusion of this toxin in Brazilian legislation for drinking-water quality control, and for the development of analytical methods for this toxin.engAcetylcholinesteraseCyanobacteriaCyanotoxinSubtropicalOccurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALOccurrence of anatoxin-a s during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil.pdfOccurrence of anatoxin-a s during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil.pdfapplication/pdf212406https://repositorio.furg.br/bitstream/1/2367/1/Occurrence%20of%20anatoxin-a%20s%20%20during%20a%20bloom%20of%20Anabaena%20crassa%20in%20a%20water-supply%20reservoir%20in%20southern%20Brazil.pdfe2ee6f5485eb2e24ca7e0d50d88dfbd7MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81678https://repositorio.furg.br/bitstream/1/2367/2/license.txtd3be63d3b3eee02729709361dac69efeMD52open access1/23672012-08-15 18:25:59.69open accessoai:repositorio.furg.br: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ório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2012-08-15T21:25:59Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
title |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
spellingShingle |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil Becker, Vanessa Acetylcholinesterase Cyanobacteria Cyanotoxin Subtropical |
title_short |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
title_full |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
title_fullStr |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
title_full_unstemmed |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
title_sort |
Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil |
author |
Becker, Vanessa |
author_facet |
Becker, Vanessa Ihara, Priscila Yunes, João Sarkis Huszar, Vera Lúcia de Moraes |
author_role |
author |
author2 |
Ihara, Priscila Yunes, João Sarkis Huszar, Vera Lúcia de Moraes |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Becker, Vanessa Ihara, Priscila Yunes, João Sarkis Huszar, Vera Lúcia de Moraes |
dc.subject.por.fl_str_mv |
Acetylcholinesterase Cyanobacteria Cyanotoxin Subtropical |
topic |
Acetylcholinesterase Cyanobacteria Cyanotoxin Subtropical |
description |
Cyanobacterial blooms and the accompanying production of cyanotoxins are a serious global problem. Toxic blooms of Anabaena species are common in lagoons and reservoirs of southern Brazil. Worldwide, species of the genus Anabaena produce the majority of the known hepatotoxins (microcystins) and neurotoxins [anatoxin-a, anatoxin-a(s), and saxitoxins]. This report links a bloom of Anabaena crassa in the Faxinal Reservoir, the main water supply for the city of Caxias do Sul (400,000 inhabitants) in southern Brazil, to the occurrence of anatoxin-a(s) in the water. During the bloom period, the reservoir was strongly stratified, with higher temperatures and a deep anoxic hypolimnion. Two methods for sample concentration (direct and complete extraction) were tested, and direct extraction of samples proved to be more efficient. Water samples collected during the bloom showed 9% acetylcholinesterase inhibition at 50 mg mL−1, corresponding to 0.61μg of anatoxin-a(s) per gram of lyophilized powder. At these concentrations, symptoms of neurotoxicity and mortality were not observed in tests with Swiss albino mice. Although the concentrations of anatoxin-a(s) in the Faxinal Reservoir were low, these results are important because this is the first record of the toxin for A. crassa. Furthermore, this cyanotoxin is not yet included in Brazilian legislation for drinking-water monitoring, because of the lack of information about toxicity levels and risk calculation for oral doses. The data presented here contribute to the basis for the future inclusion of this toxin in Brazilian legislation for drinking-water quality control, and for the development of analytical methods for this toxin. |
publishDate |
2010 |
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2010 |
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2012-08-15T21:25:59Z |
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2012-08-15T21:25:59Z |
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dc.identifier.citation.fl_str_mv |
BECKER, Vanessa et al. Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil. Journal of Applied Phycology, v.22, n. 3, p. 235-241, 2009. Disponível em: <http://www.springerlink.com/content/n135720126381k55/fulltext.pdf>. Acesso em: 08 jul. 2012. |
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http://repositorio.furg.br/handle/1/2367 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1007/s10811-009-9451-8 |
identifier_str_mv |
BECKER, Vanessa et al. Occurrence of anatoxin-a(s) during a bloom of Anabaena crassa in a water-supply reservoir in southern Brazil. Journal of Applied Phycology, v.22, n. 3, p. 235-241, 2009. Disponível em: <http://www.springerlink.com/content/n135720126381k55/fulltext.pdf>. Acesso em: 08 jul. 2012. |
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