Determination of IgG avidity in BALB/c mice experimentally infected with toxocara canis

• Autor(a) principal: Schoenardie, Elizandra Roselaine
• Data de Publicação: 2014
• Outros Autores: Scaini, Carlos James, Avila, Luciana Farias da Costa de, Sperotto, Rita Leal, Borsuk, Sibele, Felicetti, Cristine Dias Pires, Pepe, Michele, Berne, Maria Elisabeth Aires
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/7065
• topic: Visceral larva migrans; Toxocariasis; Acute and chronic infections; ELISA; Larva migrans visceral; Toxocaríase; Infecção aguda e crônica

Toxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples were collected, and sera treated with 6 M urea and assayed by ELISA every two weeks. The percent IgG avidity was determined using the mean absorbance of sera treated with urea, divided by the mean absorbance of untreated sera. In the first 15 days post-inoculation, was observed a low percentage, between 7.25 and 27.5%, IgG avidity, characteristic of an acute infection. After 60 days of infection, all the mice showed between 31.4 and 58% IgG avidity, indicating a chronic infection.