Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/7181 http://dx.doi.org/10.1590/S0036-46652015000300003 |
Resumo: | Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitroactivity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds. |
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Mata-Santos, TaísPinto, Nitza FrançaMata-Santos, Hilton AntônioMoura, Kelly Gallan deCarneiro, Paula FernandesCarvalho, Tatiane dos SantosDel Rio, Karina PenaPinto, Maria do Carmo Freire RibeiroMartins, Lourdes Helena RodriguesFenalti, Juliana MontelliSilva, Pedro Eduardo Almeida daScaini, Carlos James2017-05-22T15:54:14Z2017-05-22T15:54:14Z2015MATA-SANTOS, Taís et al. Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 3, p. 197-204, 2015. Disponível em:< http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000300197>. Acesso em: 20 mar. 2017.0036-4665O1678-9946http://repositorio.furg.br/handle/1/7181http://dx.doi.org/10.1590/S0036-46652015000300003Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitroactivity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds.engToxocara canisQuinonesChemotherapyAnthelminticsAnthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINAL0036-4665-rimtsp-57-03-0197.pdf0036-4665-rimtsp-57-03-0197.pdfapplication/pdf176595https://repositorio.furg.br/bitstream/1/7181/1/0036-4665-rimtsp-57-03-0197.pdf03cbf4d42c80cc5a870b341e0b4b7005MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/7181/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/71812019-11-22 10:18:21.08open accessoai:repositorio.furg.br:1/7181Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-11-22T13:18:21Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
title |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
spellingShingle |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae Mata-Santos, Taís Toxocara canis Quinones Chemotherapy Anthelmintics |
title_short |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
title_full |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
title_fullStr |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
title_full_unstemmed |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
title_sort |
Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae |
author |
Mata-Santos, Taís |
author_facet |
Mata-Santos, Taís Pinto, Nitza França Mata-Santos, Hilton Antônio Moura, Kelly Gallan de Carneiro, Paula Fernandes Carvalho, Tatiane dos Santos Del Rio, Karina Pena Pinto, Maria do Carmo Freire Ribeiro Martins, Lourdes Helena Rodrigues Fenalti, Juliana Montelli Silva, Pedro Eduardo Almeida da Scaini, Carlos James |
author_role |
author |
author2 |
Pinto, Nitza França Mata-Santos, Hilton Antônio Moura, Kelly Gallan de Carneiro, Paula Fernandes Carvalho, Tatiane dos Santos Del Rio, Karina Pena Pinto, Maria do Carmo Freire Ribeiro Martins, Lourdes Helena Rodrigues Fenalti, Juliana Montelli Silva, Pedro Eduardo Almeida da Scaini, Carlos James |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mata-Santos, Taís Pinto, Nitza França Mata-Santos, Hilton Antônio Moura, Kelly Gallan de Carneiro, Paula Fernandes Carvalho, Tatiane dos Santos Del Rio, Karina Pena Pinto, Maria do Carmo Freire Ribeiro Martins, Lourdes Helena Rodrigues Fenalti, Juliana Montelli Silva, Pedro Eduardo Almeida da Scaini, Carlos James |
dc.subject.por.fl_str_mv |
Toxocara canis Quinones Chemotherapy Anthelmintics |
topic |
Toxocara canis Quinones Chemotherapy Anthelmintics |
description |
Anthelmintics used for intestinal helminthiasis treatment are generally effective; however, their effectiveness in tissue parasitosis (i.e. visceral toxocariasis) is moderate. The aim of this study was to evaluate the in vitroactivity of lapachol, β-lapachone and phenazines in relation to the viability of Toxocara canis larvae. A concentration of 2 mg/mL (in duplicate) of the compounds was tested using microculture plates containing Toxocara canis larvae in an RPMI-1640 environment, incubated at 37 °C in 5% CO2 tension for 48 hours. In the 2 mg/mL concentration, four phenazines, lapachol and three of its derivatives presented a larvicide/larvistatic activity of 100%. Then, the minimum larvicide/larvistatic concentration (MLC) test was conducted. The compounds that presented the best results were nor-lapachol (MLC, 1 mg/mL), lapachol (MLC 0.5 mg/mL), β-lapachone, and β-C-allyl-lawsone (MLC, 0.25 mg/mL). The larvae exposed to the compounds, at best MLC with 100% in vitro activity larvicide, were inoculated into healthy BALB/c mice and were not capable of causing infection, confirming the larvicide potential in vitro of these compounds. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2017-05-22T15:54:14Z |
dc.date.available.fl_str_mv |
2017-05-22T15:54:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MATA-SANTOS, Taís et al. Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 3, p. 197-204, 2015. Disponível em:< http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000300197>. Acesso em: 20 mar. 2017. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/7181 |
dc.identifier.issn.none.fl_str_mv |
0036-4665O 1678-9946 |
dc.identifier.doi.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/S0036-46652015000300003 |
identifier_str_mv |
MATA-SANTOS, Taís et al. Anthelmintic activity of lapachol, β-lapachone and its derivates againts Toxocara canis larvae. Revista do Instituto de Medicina Tropical de São Paulo, v. 57, n. 3, p. 197-204, 2015. Disponível em:< http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000300197>. Acesso em: 20 mar. 2017. 0036-4665O 1678-9946 |
url |
http://repositorio.furg.br/handle/1/7181 http://dx.doi.org/10.1590/S0036-46652015000300003 |
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eng |
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eng |
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openAccess |
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reponame:Repositório Institucional da FURG (RI FURG) instname:Universidade Federal do Rio Grande (FURG) instacron:FURG |
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Universidade Federal do Rio Grande (FURG) |
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