Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4842 |
Resumo: | ASA (acetylsalicylic acid) is an NSAID (non-steroidal anti-inflammatory drug). ASA has gained attention as a potential chemopreventive and chemotherapeutic agent for several neoplasms. The aim of this study was to analyse the possible antitumoural effects of ASA in two erythroleukaemic cell lines, with or without the MDR (multidrug resistance) phenotype. The mechanism of action of different concentrations of ASA were compared in K562 (non-MDR) and Lucena (MDR) cells by analysing cell viability, apoptosis and necrosis, intracellular ROS (reactive oxygen species) formation and bcl-2, p53 and cox-2 gene expression. ASA inhibited the cellular proliferation or induced toxicity in K562 and Lucena cell lines, irrespective of the MDR phenotype. The ASA treatment provoked death by apoptosis and necrosis in K562 cells and only by necrosis in Lucena cells. ASA also showed antioxidant activity in both cell lines. The bcl-2, p53 and cox-2 genes in both cell lines treated with ASA seem to exhibit different patterns of expression. However, normal lymphocytes treated with the same ASA concentrations were more resistant than tumoral cells. The results of this work show that both cell lines responded to treatment with ASA, demonstrating a possible antitumoral and anti-MDR role for this drug |
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Carrett-Dias, MicheleVotto, Ana Paula de SouzaFilgueira, Daza de Moraes Vaz BatistaAlmeida, Daniela VolcanVallochi, Adriana LimaD'Oca, Marcelo Gonçalves MontesMarins, Luis Fernando FernandesTrindade, Gilma Santos2015-05-14T20:22:35Z2015-05-14T20:22:35Z2011CARRETT-DIAS, Michele et al. Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells. Bioscience Reports, v. 31 n. 5, p. 391-398, 2011. Disponível em: <http://www.bioscirep.org/bsr/031/0391/0310391.pdf>. Acesso em 07 mai. 20151573-4935http://repositorio.furg.br/handle/1/484210.1042/BSR20100115ASA (acetylsalicylic acid) is an NSAID (non-steroidal anti-inflammatory drug). ASA has gained attention as a potential chemopreventive and chemotherapeutic agent for several neoplasms. The aim of this study was to analyse the possible antitumoural effects of ASA in two erythroleukaemic cell lines, with or without the MDR (multidrug resistance) phenotype. The mechanism of action of different concentrations of ASA were compared in K562 (non-MDR) and Lucena (MDR) cells by analysing cell viability, apoptosis and necrosis, intracellular ROS (reactive oxygen species) formation and bcl-2, p53 and cox-2 gene expression. ASA inhibited the cellular proliferation or induced toxicity in K562 and Lucena cell lines, irrespective of the MDR phenotype. The ASA treatment provoked death by apoptosis and necrosis in K562 cells and only by necrosis in Lucena cells. ASA also showed antioxidant activity in both cell lines. The bcl-2, p53 and cox-2 genes in both cell lines treated with ASA seem to exhibit different patterns of expression. However, normal lymphocytes treated with the same ASA concentrations were more resistant than tumoral cells. The results of this work show that both cell lines responded to treatment with ASA, demonstrating a possible antitumoral and anti-MDR role for this drugengAcetylsalicylic acid (ASA)AntioxidantDeath cellGene expressionLeukaemiaMultidrug resistance (MDR)Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALnti-MDR and antitumoral action of acetylsalicylic.pdfnti-MDR and antitumoral action of acetylsalicylic.pdfapplication/pdf596975https://repositorio.furg.br/bitstream/1/4842/1/nti-MDR%20and%20antitumoral%20action%20of%20acetylsalicylic.pdf35fdc269c355bb40181c525cd5f505e4MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/4842/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/48422019-11-22 10:04:36.48open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-11-22T13:04:36Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
title |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
spellingShingle |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells Carrett-Dias, Michele Acetylsalicylic acid (ASA) Antioxidant Death cell Gene expression Leukaemia Multidrug resistance (MDR) |
title_short |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
title_full |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
title_fullStr |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
title_full_unstemmed |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
title_sort |
Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells |
author |
Carrett-Dias, Michele |
author_facet |
Carrett-Dias, Michele Votto, Ana Paula de Souza Filgueira, Daza de Moraes Vaz Batista Almeida, Daniela Volcan Vallochi, Adriana Lima D'Oca, Marcelo Gonçalves Montes Marins, Luis Fernando Fernandes Trindade, Gilma Santos |
author_role |
author |
author2 |
Votto, Ana Paula de Souza Filgueira, Daza de Moraes Vaz Batista Almeida, Daniela Volcan Vallochi, Adriana Lima D'Oca, Marcelo Gonçalves Montes Marins, Luis Fernando Fernandes Trindade, Gilma Santos |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Carrett-Dias, Michele Votto, Ana Paula de Souza Filgueira, Daza de Moraes Vaz Batista Almeida, Daniela Volcan Vallochi, Adriana Lima D'Oca, Marcelo Gonçalves Montes Marins, Luis Fernando Fernandes Trindade, Gilma Santos |
dc.subject.por.fl_str_mv |
Acetylsalicylic acid (ASA) Antioxidant Death cell Gene expression Leukaemia Multidrug resistance (MDR) |
topic |
Acetylsalicylic acid (ASA) Antioxidant Death cell Gene expression Leukaemia Multidrug resistance (MDR) |
description |
ASA (acetylsalicylic acid) is an NSAID (non-steroidal anti-inflammatory drug). ASA has gained attention as a potential chemopreventive and chemotherapeutic agent for several neoplasms. The aim of this study was to analyse the possible antitumoural effects of ASA in two erythroleukaemic cell lines, with or without the MDR (multidrug resistance) phenotype. The mechanism of action of different concentrations of ASA were compared in K562 (non-MDR) and Lucena (MDR) cells by analysing cell viability, apoptosis and necrosis, intracellular ROS (reactive oxygen species) formation and bcl-2, p53 and cox-2 gene expression. ASA inhibited the cellular proliferation or induced toxicity in K562 and Lucena cell lines, irrespective of the MDR phenotype. The ASA treatment provoked death by apoptosis and necrosis in K562 cells and only by necrosis in Lucena cells. ASA also showed antioxidant activity in both cell lines. The bcl-2, p53 and cox-2 genes in both cell lines treated with ASA seem to exhibit different patterns of expression. However, normal lymphocytes treated with the same ASA concentrations were more resistant than tumoral cells. The results of this work show that both cell lines responded to treatment with ASA, demonstrating a possible antitumoral and anti-MDR role for this drug |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2015-05-14T20:22:35Z |
dc.date.available.fl_str_mv |
2015-05-14T20:22:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARRETT-DIAS, Michele et al. Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells. Bioscience Reports, v. 31 n. 5, p. 391-398, 2011. Disponível em: <http://www.bioscirep.org/bsr/031/0391/0310391.pdf>. Acesso em 07 mai. 2015 |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/4842 |
dc.identifier.issn.none.fl_str_mv |
1573-4935 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1042/BSR20100115 |
identifier_str_mv |
CARRETT-DIAS, Michele et al. Anti-MDR and antitumoral action of acetylsalicylic acid on leukaemic cells. Bioscience Reports, v. 31 n. 5, p. 391-398, 2011. Disponível em: <http://www.bioscirep.org/bsr/031/0391/0310391.pdf>. Acesso em 07 mai. 2015 1573-4935 10.1042/BSR20100115 |
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http://repositorio.furg.br/handle/1/4842 |
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