In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae)
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Iheringia. Série Botânica |
Texto Completo: | https://isb.emnuvens.com.br/iheringia/article/view/128 |
Resumo: | Vriesea scalaris is an ornamental bromeliad. The overall aim of this research was to establish conditions for in vitro multiplication and growth. Seeds were disinfected and in vitro germinated on MS medium containing 0, 0.5, 1, 2, or 4.5 ìM BAP (6-benzylaminopurine) or KIN (6-furfurylaminopurine or kinetin). Rooting was achieved on half strength MS supplemented with 0, 2.5, 5, or 10 ìM IBA (indole-3-butyric acid). Some seedlings (thirty-day-old) were kept on MS medium in the presence of 10 mL.L-1 B5 vitamins and others in the absence. Leaf explants were cultured on MSL medium (MS salts and vitamins, 30 g.L-1 sucrose, 0.15 g.L-1 ascorbic acid, 0.4 ìM BAP, 0.5 ìM NAA (alpha-naphthaleneacetic acid) and solidifi ed with 6 g.L-1 agar) and submitted o the treatments: MSL medium (control); MSL + 0.06g.L-1 NH4NO3; MSL + 0.25g.L-1 hydrolyzed casein or MSL + 2mL.L-1 Fuji vitamins. BAP was more suitable than KIN for shoot multiplication. IBA at the concentrations used induced low rooting, the absence of IBA promoted a better rooting rate. The B5 vitamins promoted an increase in multiplication rate. The results of these experiments demonstrated the viability of the in vitro multiplication for this species. Seedlings and leaf explants showed the same multiplication rate (ca. 8 shoots per explant). These protocols can be used for in vitro propagation and for in vitro germplasm conservation. |
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In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae)Multiplicação in vitro de Vriesea scalaris E. Morrem (Bromeliaceae).organogenesisbromeliadstissue cultureplant growth regulators.organogenêsebroméliascultivo de tecidosreguladores de crescimento.Vriesea scalaris is an ornamental bromeliad. The overall aim of this research was to establish conditions for in vitro multiplication and growth. Seeds were disinfected and in vitro germinated on MS medium containing 0, 0.5, 1, 2, or 4.5 ìM BAP (6-benzylaminopurine) or KIN (6-furfurylaminopurine or kinetin). Rooting was achieved on half strength MS supplemented with 0, 2.5, 5, or 10 ìM IBA (indole-3-butyric acid). Some seedlings (thirty-day-old) were kept on MS medium in the presence of 10 mL.L-1 B5 vitamins and others in the absence. Leaf explants were cultured on MSL medium (MS salts and vitamins, 30 g.L-1 sucrose, 0.15 g.L-1 ascorbic acid, 0.4 ìM BAP, 0.5 ìM NAA (alpha-naphthaleneacetic acid) and solidifi ed with 6 g.L-1 agar) and submitted o the treatments: MSL medium (control); MSL + 0.06g.L-1 NH4NO3; MSL + 0.25g.L-1 hydrolyzed casein or MSL + 2mL.L-1 Fuji vitamins. BAP was more suitable than KIN for shoot multiplication. IBA at the concentrations used induced low rooting, the absence of IBA promoted a better rooting rate. The B5 vitamins promoted an increase in multiplication rate. The results of these experiments demonstrated the viability of the in vitro multiplication for this species. Seedlings and leaf explants showed the same multiplication rate (ca. 8 shoots per explant). These protocols can be used for in vitro propagation and for in vitro germplasm conservation.Vriesea scalaris é uma bromélia ornamental. O objetivo desta pesquisa foi estabelecer condições para multiplicação e crescimento in vitro. Sementes foram desinfestadas e germinadas in vitro em meio MS contendo 0; 0,5; 1; 2 ou 4,5 ìM de BAP (6-benzilaminopurina) ou KIN (6-furfurilaminopurina ou cinetina). O enraizamento in vitro foi induzido em meio MS adicionado de 0; 2,5; 5 ou 10 ìM de IBA (ácido indolbutírico). Plântulas com 30 dias foram cultivadas na presença ou ausência de 10mL.L-1 de vitaminas B5. Explantes foliares foram cultivados em meio MSL (MS sais e vitaminhas, 30 g.L-1 de sacarose, 0,15 g.L-1 de ácido ascórbico, 0,4 ìM de BAP, 0,5 ìM de ANA (ácido naftalenoacéito) e solidifi cado com 6 g.L-1 agar), meio MSL + 0,06g.L-1 de NH4NO3; MSL + 0,25g.L-1 de caseína hidrolisada ou MSL + 2mL.L-1 de vitaminas Fuji. O BAP foi mais satisfatório do que KIN para a multiplicação dos brotos. O enraizamento in vitro foi obtido na ausência de IBA. As vitaminas B5 promoveram um aumento na taxa de multiplicação de brotos. Plântulas e explantes foliares apresentaram a mesma taxa de multiplicação, cerca de 8 brotos por explante. Os resultados destes experimentos demonstraram a viabilidade da multiplicação in vitro para esta espécie, podendo ser utilizados para a propagação in vitro e conservação de germoplasma in vitro.Iheringia. Série Botânica.Iheringia, Série Botânica.2009-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://isb.emnuvens.com.br/iheringia/article/view/128Iheringia. Série Botânica.; Vol. 64 No. 2 (2009); 151-155Iheringia, Série Botânica.; v. 64 n. 2 (2009); 151-1552446-82310073-4705reponame:Iheringia. Série Botânicainstname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)instacron:FZB/RSenghttps://isb.emnuvens.com.br/iheringia/article/view/128/135Copyright (c) 2015 Iheringia. Série Botânica.info:eu-repo/semantics/openAccessSilva, André Luís Lopes daFranco, Elci Terezinha HenzDornelles, Eduardo BortoluzziBortoli, Caroline Larissa ReichertQuoirin, Marguerite2016-07-06T20:02:12Zoai:ojs.emnuvens.com.br:article/128Revistahttps://isb.emnuvens.com.br/iheringiaONGhttps://isb.emnuvens.com.br/iheringia/oai||iheringia-bot@fzb.rs.gov.br2446-82310073-4705opendoar:2016-07-06T20:02:12Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)false |
dc.title.none.fl_str_mv |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) Multiplicação in vitro de Vriesea scalaris E. Morrem (Bromeliaceae). |
title |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
spellingShingle |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) Silva, André Luís Lopes da organogenesis bromeliads tissue culture plant growth regulators. organogenêse bromélias cultivo de tecidos reguladores de crescimento. |
title_short |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
title_full |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
title_fullStr |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
title_full_unstemmed |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
title_sort |
In vitro multiplication of Vriesea scalaris E. Morren (Bromeliaceae) |
author |
Silva, André Luís Lopes da |
author_facet |
Silva, André Luís Lopes da Franco, Elci Terezinha Henz Dornelles, Eduardo Bortoluzzi Bortoli, Caroline Larissa Reichert Quoirin, Marguerite |
author_role |
author |
author2 |
Franco, Elci Terezinha Henz Dornelles, Eduardo Bortoluzzi Bortoli, Caroline Larissa Reichert Quoirin, Marguerite |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, André Luís Lopes da Franco, Elci Terezinha Henz Dornelles, Eduardo Bortoluzzi Bortoli, Caroline Larissa Reichert Quoirin, Marguerite |
dc.subject.por.fl_str_mv |
organogenesis bromeliads tissue culture plant growth regulators. organogenêse bromélias cultivo de tecidos reguladores de crescimento. |
topic |
organogenesis bromeliads tissue culture plant growth regulators. organogenêse bromélias cultivo de tecidos reguladores de crescimento. |
description |
Vriesea scalaris is an ornamental bromeliad. The overall aim of this research was to establish conditions for in vitro multiplication and growth. Seeds were disinfected and in vitro germinated on MS medium containing 0, 0.5, 1, 2, or 4.5 ìM BAP (6-benzylaminopurine) or KIN (6-furfurylaminopurine or kinetin). Rooting was achieved on half strength MS supplemented with 0, 2.5, 5, or 10 ìM IBA (indole-3-butyric acid). Some seedlings (thirty-day-old) were kept on MS medium in the presence of 10 mL.L-1 B5 vitamins and others in the absence. Leaf explants were cultured on MSL medium (MS salts and vitamins, 30 g.L-1 sucrose, 0.15 g.L-1 ascorbic acid, 0.4 ìM BAP, 0.5 ìM NAA (alpha-naphthaleneacetic acid) and solidifi ed with 6 g.L-1 agar) and submitted o the treatments: MSL medium (control); MSL + 0.06g.L-1 NH4NO3; MSL + 0.25g.L-1 hydrolyzed casein or MSL + 2mL.L-1 Fuji vitamins. BAP was more suitable than KIN for shoot multiplication. IBA at the concentrations used induced low rooting, the absence of IBA promoted a better rooting rate. The B5 vitamins promoted an increase in multiplication rate. The results of these experiments demonstrated the viability of the in vitro multiplication for this species. Seedlings and leaf explants showed the same multiplication rate (ca. 8 shoots per explant). These protocols can be used for in vitro propagation and for in vitro germplasm conservation. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://isb.emnuvens.com.br/iheringia/article/view/128 |
url |
https://isb.emnuvens.com.br/iheringia/article/view/128 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://isb.emnuvens.com.br/iheringia/article/view/128/135 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Iheringia. Série Botânica. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Iheringia. Série Botânica. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Iheringia. Série Botânica. Iheringia, Série Botânica. |
publisher.none.fl_str_mv |
Iheringia. Série Botânica. Iheringia, Série Botânica. |
dc.source.none.fl_str_mv |
Iheringia. Série Botânica.; Vol. 64 No. 2 (2009); 151-155 Iheringia, Série Botânica.; v. 64 n. 2 (2009); 151-155 2446-8231 0073-4705 reponame:Iheringia. Série Botânica instname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS) instacron:FZB/RS |
instname_str |
Fundação Zoobotânica do Rio Grande do Sul (FZB/RS) |
instacron_str |
FZB/RS |
institution |
FZB/RS |
reponame_str |
Iheringia. Série Botânica |
collection |
Iheringia. Série Botânica |
repository.name.fl_str_mv |
Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS) |
repository.mail.fl_str_mv |
||iheringia-bot@fzb.rs.gov.br |
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1798948134761529344 |