Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds

Detalhes bibliográficos
Autor(a) principal: Bandeira, Juliana de Magalhães
Data de Publicação: 2014
Outros Autores: Marini, Patrícia, Borba, Isabel Cristina Gouvea de, Martins, Andrea Bicca Noguez, Amarante, Luciano do, Moraes, Dario Munt de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Iheringia. Série Botânica
Texto Completo: https://isb.emnuvens.com.br/iheringia/article/view/92
Resumo: We aimed to differentiate the vigor of soybean seeds through antioxidant enzyme activity (superoxide dismutase-SOD, ascorbate peroxidase-APX, and catalase- CAT) and relate them to the viability and vigor standard tests to verify if these enzymes detect tenuous differences in seed vigor. The following tests were performed: germination, fi rst germination count, germination speed index, initial growth of seedlings, electrical conductivity, and respiratory and enzymatic activity. It was only possible to differentiate just two lots of soybean seeds by vigor and germination test, considering that viability had higher germination in lot 2 (91%), which did not differ from lot 3 (87.17%) nor from lot 1 (85.17%), while SOD and APX enzymes activity, in the roots, differed amongst the three lots. Thus, it was concluded that the viability and vigor standard tests relate to antioxidant enzyme activity, characterizing this method as an effi cient tool for detecting small differences in the vigor of soybean seeds.
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spelling Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seedsMetabolismo antioxidativo: uma ferramenta para detectar pequenas diferenças no vigor de sementes de soja.ascorbate peroxidasecatalasephysiological qualitysuperoxide dismutaseascorbato peroxidasecatalasequalidade fi siológicasuperóxido dismutaseWe aimed to differentiate the vigor of soybean seeds through antioxidant enzyme activity (superoxide dismutase-SOD, ascorbate peroxidase-APX, and catalase- CAT) and relate them to the viability and vigor standard tests to verify if these enzymes detect tenuous differences in seed vigor. The following tests were performed: germination, fi rst germination count, germination speed index, initial growth of seedlings, electrical conductivity, and respiratory and enzymatic activity. It was only possible to differentiate just two lots of soybean seeds by vigor and germination test, considering that viability had higher germination in lot 2 (91%), which did not differ from lot 3 (87.17%) nor from lot 1 (85.17%), while SOD and APX enzymes activity, in the roots, differed amongst the three lots. Thus, it was concluded that the viability and vigor standard tests relate to antioxidant enzyme activity, characterizing this method as an effi cient tool for detecting small differences in the vigor of soybean seeds.Objetivou-se diferenciar o vigor de sementes de soja por meio da atividade das enzimas antioxidantes (superóxido dismutase-SOD, ascorbato peroxidase-APX e catalase-CAT) e relacioná-las aos testes padrão de viabilidade e vigor, tendo por fi nalidade verifi car se estas enzimas detectam diferenças tênues no vigor das sementes. Foram realizados os seguintes testes: germinação, primeira contagem e índice de velocidade de germinação, crescimento inicial de plântulas, condutividade elétrica, atividade respiratória e enzimática. Foi possível diferenciar apenas dois lotes de sementes de soja pelos testes de vigor e germinação, sendo que a viabilidade apresentou maior germinação para o lote 2 (91%), não diferindo do lote 3 (87,17%), diferindo do lote 1 (85,17%), enquanto que a atividade das enzimas SOD e APX, nas raízes, diferenciou as sementes em três lotes. Portanto, conclui-se que os testes padrão de viabilidade e vigor têm relação com a atividade das enzimas antioxidantes, caracterizando este método como uma ferramenta efi ciente para detectar pequenas diferenças no vigor de sementes de soja.Iheringia. Série Botânica.Iheringia, Série Botânica.2014-12-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://isb.emnuvens.com.br/iheringia/article/view/92Iheringia. Série Botânica.; Vol. 69 No. 2 (2014); 285-292Iheringia, Série Botânica.; v. 69 n. 2 (2014); 285-2922446-82310073-4705reponame:Iheringia. Série Botânicainstname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)instacron:FZB/RSenghttps://isb.emnuvens.com.br/iheringia/article/view/92/99Copyright (c) 2015 Iheringia. Série Botânica.info:eu-repo/semantics/openAccessBandeira, Juliana de MagalhãesMarini, PatríciaBorba, Isabel Cristina Gouvea deMartins, Andrea Bicca NoguezAmarante, Luciano doMoraes, Dario Munt de2016-07-06T20:02:07Zoai:ojs.emnuvens.com.br:article/92Revistahttps://isb.emnuvens.com.br/iheringiaONGhttps://isb.emnuvens.com.br/iheringia/oai||iheringia-bot@fzb.rs.gov.br2446-82310073-4705opendoar:2016-07-06T20:02:07Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)false
dc.title.none.fl_str_mv Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
Metabolismo antioxidativo: uma ferramenta para detectar pequenas diferenças no vigor de sementes de soja.
title Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
spellingShingle Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
Bandeira, Juliana de Magalhães
ascorbate peroxidase
catalase
physiological quality
superoxide dismutase
ascorbato peroxidase
catalase
qualidade fi siológica
superóxido dismutase
title_short Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
title_full Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
title_fullStr Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
title_full_unstemmed Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
title_sort Antioxidative metabolism: a tool to detect small differences in the vigor of soybean seeds
author Bandeira, Juliana de Magalhães
author_facet Bandeira, Juliana de Magalhães
Marini, Patrícia
Borba, Isabel Cristina Gouvea de
Martins, Andrea Bicca Noguez
Amarante, Luciano do
Moraes, Dario Munt de
author_role author
author2 Marini, Patrícia
Borba, Isabel Cristina Gouvea de
Martins, Andrea Bicca Noguez
Amarante, Luciano do
Moraes, Dario Munt de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bandeira, Juliana de Magalhães
Marini, Patrícia
Borba, Isabel Cristina Gouvea de
Martins, Andrea Bicca Noguez
Amarante, Luciano do
Moraes, Dario Munt de
dc.subject.por.fl_str_mv ascorbate peroxidase
catalase
physiological quality
superoxide dismutase
ascorbato peroxidase
catalase
qualidade fi siológica
superóxido dismutase
topic ascorbate peroxidase
catalase
physiological quality
superoxide dismutase
ascorbato peroxidase
catalase
qualidade fi siológica
superóxido dismutase
description We aimed to differentiate the vigor of soybean seeds through antioxidant enzyme activity (superoxide dismutase-SOD, ascorbate peroxidase-APX, and catalase- CAT) and relate them to the viability and vigor standard tests to verify if these enzymes detect tenuous differences in seed vigor. The following tests were performed: germination, fi rst germination count, germination speed index, initial growth of seedlings, electrical conductivity, and respiratory and enzymatic activity. It was only possible to differentiate just two lots of soybean seeds by vigor and germination test, considering that viability had higher germination in lot 2 (91%), which did not differ from lot 3 (87.17%) nor from lot 1 (85.17%), while SOD and APX enzymes activity, in the roots, differed amongst the three lots. Thus, it was concluded that the viability and vigor standard tests relate to antioxidant enzyme activity, characterizing this method as an effi cient tool for detecting small differences in the vigor of soybean seeds.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/92
url https://isb.emnuvens.com.br/iheringia/article/view/92
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://isb.emnuvens.com.br/iheringia/article/view/92/99
dc.rights.driver.fl_str_mv Copyright (c) 2015 Iheringia. Série Botânica.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Iheringia. Série Botânica.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
publisher.none.fl_str_mv Iheringia. Série Botânica.
Iheringia, Série Botânica.
dc.source.none.fl_str_mv Iheringia. Série Botânica.; Vol. 69 No. 2 (2014); 285-292
Iheringia, Série Botânica.; v. 69 n. 2 (2014); 285-292
2446-8231
0073-4705
reponame:Iheringia. Série Botânica
instname:Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron:FZB/RS
instname_str Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
instacron_str FZB/RS
institution FZB/RS
reponame_str Iheringia. Série Botânica
collection Iheringia. Série Botânica
repository.name.fl_str_mv Iheringia. Série Botânica - Fundação Zoobotânica do Rio Grande do Sul (FZB/RS)
repository.mail.fl_str_mv ||iheringia-bot@fzb.rs.gov.br
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