Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Summa phytopathologica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052019000100033 |
Resumo: | ABSTRACT The fungi Colletotrichum truncatum and Macrophomina phaseolina are among the main pathogens associated with lima bean seeds in Brazil, and they are the causal agents of anthracnose and charcoal rot, respectively. The aim of this study was to verify the seed-seedling transmission of C. truncatum and M. phaseolina in artificially inoculated lima bean seeds. Potato-dextrose-agar (PDA) media with different hydric potentials were obtained after the addition of mannitol. Isolates of C. truncatum and M. phaseolina were peaked to these media. Forty grams of seeds were distributed over the fungal mycelium, remaining there for different exposure periods, as a function of each treatment: T1: 0.0 MPa without inoculum for 48 h; T2: 0.0 MPa for 48 h; T3: -0.2 MPa for 48 h; T4: -0.4 MPa for 48 h; T5: -0.6 MPa for 72 h; T6: -0.8 MPa for 96 h and T7: -1.0 MPa for 120 h. The seeds were incubated at 25 ºC under a 12-hour photoperiod. Germination in a paper roll, emergence of seedlings in sand and seed health in PDA were evaluated. T4 provided the best conditions to temporarily prevent seed germination, promoting a greater incidence of seeds infected by C. truncatum and a greater percentage of diseased plants. M. phaseolina reduced seed germination, independently of the inoculated treatment. Both fungi were transmitted from the seeds to the seedlings of lima bean, causing symptoms in various aerial parts. C. truncatum and M. phaseolina transmission rate was 83.1% and 76.46%, respectively. |
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Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seedsGerminationPhaseolus lunatusseed healthtransmission by seedsABSTRACT The fungi Colletotrichum truncatum and Macrophomina phaseolina are among the main pathogens associated with lima bean seeds in Brazil, and they are the causal agents of anthracnose and charcoal rot, respectively. The aim of this study was to verify the seed-seedling transmission of C. truncatum and M. phaseolina in artificially inoculated lima bean seeds. Potato-dextrose-agar (PDA) media with different hydric potentials were obtained after the addition of mannitol. Isolates of C. truncatum and M. phaseolina were peaked to these media. Forty grams of seeds were distributed over the fungal mycelium, remaining there for different exposure periods, as a function of each treatment: T1: 0.0 MPa without inoculum for 48 h; T2: 0.0 MPa for 48 h; T3: -0.2 MPa for 48 h; T4: -0.4 MPa for 48 h; T5: -0.6 MPa for 72 h; T6: -0.8 MPa for 96 h and T7: -1.0 MPa for 120 h. The seeds were incubated at 25 ºC under a 12-hour photoperiod. Germination in a paper roll, emergence of seedlings in sand and seed health in PDA were evaluated. T4 provided the best conditions to temporarily prevent seed germination, promoting a greater incidence of seeds infected by C. truncatum and a greater percentage of diseased plants. M. phaseolina reduced seed germination, independently of the inoculated treatment. Both fungi were transmitted from the seeds to the seedlings of lima bean, causing symptoms in various aerial parts. C. truncatum and M. phaseolina transmission rate was 83.1% and 76.46%, respectively.Grupo Paulista de Fitopatologia2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052019000100033Summa Phytopathologica v.45 n.1 2019reponame:Summa phytopathologica (Online)instname:Grupo Paulista de Fitopatologiainstacron:GPF10.1590/0100-5405/180868info:eu-repo/semantics/openAccessMota,José Monteiro daMelo,Maruzanete Pereira deGarcía,Mynor Efraín MunguíaSousa,Enayra SilvaSousa,Emanoela Maria de JesusBarguil,Beatriz MeirelesBeserra Jr,José Evando Aguiareng2019-03-29T00:00:00Zoai:scielo:S0100-54052019000100033Revistahttp://www.scielo.br/sphttps://old.scielo.br/oai/scielo-oai.phpsumma@fca.unesp.br1980-54540100-5405opendoar:2019-03-29T00:00Summa phytopathologica (Online) - Grupo Paulista de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
title |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
spellingShingle |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds Mota,José Monteiro da Germination Phaseolus lunatus seed health transmission by seeds |
title_short |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
title_full |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
title_fullStr |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
title_full_unstemmed |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
title_sort |
Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds |
author |
Mota,José Monteiro da |
author_facet |
Mota,José Monteiro da Melo,Maruzanete Pereira de García,Mynor Efraín Munguía Sousa,Enayra Silva Sousa,Emanoela Maria de Jesus Barguil,Beatriz Meireles Beserra Jr,José Evando Aguiar |
author_role |
author |
author2 |
Melo,Maruzanete Pereira de García,Mynor Efraín Munguía Sousa,Enayra Silva Sousa,Emanoela Maria de Jesus Barguil,Beatriz Meireles Beserra Jr,José Evando Aguiar |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Mota,José Monteiro da Melo,Maruzanete Pereira de García,Mynor Efraín Munguía Sousa,Enayra Silva Sousa,Emanoela Maria de Jesus Barguil,Beatriz Meireles Beserra Jr,José Evando Aguiar |
dc.subject.por.fl_str_mv |
Germination Phaseolus lunatus seed health transmission by seeds |
topic |
Germination Phaseolus lunatus seed health transmission by seeds |
description |
ABSTRACT The fungi Colletotrichum truncatum and Macrophomina phaseolina are among the main pathogens associated with lima bean seeds in Brazil, and they are the causal agents of anthracnose and charcoal rot, respectively. The aim of this study was to verify the seed-seedling transmission of C. truncatum and M. phaseolina in artificially inoculated lima bean seeds. Potato-dextrose-agar (PDA) media with different hydric potentials were obtained after the addition of mannitol. Isolates of C. truncatum and M. phaseolina were peaked to these media. Forty grams of seeds were distributed over the fungal mycelium, remaining there for different exposure periods, as a function of each treatment: T1: 0.0 MPa without inoculum for 48 h; T2: 0.0 MPa for 48 h; T3: -0.2 MPa for 48 h; T4: -0.4 MPa for 48 h; T5: -0.6 MPa for 72 h; T6: -0.8 MPa for 96 h and T7: -1.0 MPa for 120 h. The seeds were incubated at 25 ºC under a 12-hour photoperiod. Germination in a paper roll, emergence of seedlings in sand and seed health in PDA were evaluated. T4 provided the best conditions to temporarily prevent seed germination, promoting a greater incidence of seeds infected by C. truncatum and a greater percentage of diseased plants. M. phaseolina reduced seed germination, independently of the inoculated treatment. Both fungi were transmitted from the seeds to the seedlings of lima bean, causing symptoms in various aerial parts. C. truncatum and M. phaseolina transmission rate was 83.1% and 76.46%, respectively. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052019000100033 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052019000100033 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0100-5405/180868 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
publisher.none.fl_str_mv |
Grupo Paulista de Fitopatologia |
dc.source.none.fl_str_mv |
Summa Phytopathologica v.45 n.1 2019 reponame:Summa phytopathologica (Online) instname:Grupo Paulista de Fitopatologia instacron:GPF |
instname_str |
Grupo Paulista de Fitopatologia |
instacron_str |
GPF |
institution |
GPF |
reponame_str |
Summa phytopathologica (Online) |
collection |
Summa phytopathologica (Online) |
repository.name.fl_str_mv |
Summa phytopathologica (Online) - Grupo Paulista de Fitopatologia |
repository.mail.fl_str_mv |
summa@fca.unesp.br |
_version_ |
1754193419260919808 |