Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1

Detalhes bibliográficos
Autor(a) principal: Ferreira,Flaviane Lopes
Data de Publicação: 2018
Outros Autores: Dall'Antonia,Cesar Barretta, Shiga,Emerson Andrade, Alvim,Larissa Juliani, Pessoni,Rosemeire Aparecida Bom
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hoehnea
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134
Resumo: ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.
id IBT-1_6947470474d5abd3c540505a57003cfb
oai_identifier_str oai:scielo:S2236-89062018000100134
network_acronym_str IBT-1
network_name_str Hoehnea
repository_id_str
spelling Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1anamorphic fungiAspergillus japonicusendo-β-1,4-xylanaseenzymeslaccaseβ-xylosidaseABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.Instituto de Pesquisas Ambientais2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134Hoehnea v.45 n.1 2018reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-40/2017info:eu-repo/semantics/openAccessFerreira,Flaviane LopesDall'Antonia,Cesar BarrettaShiga,Emerson AndradeAlvim,Larissa JulianiPessoni,Rosemeire Aparecida Bomeng2018-04-23T00:00:00Zoai:scielo:S2236-89062018000100134Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2018-04-23T00:00Hoehnea - Instituto de Botânica (IBT)false
dc.title.none.fl_str_mv Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
title Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
spellingShingle Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
Ferreira,Flaviane Lopes
anamorphic fungi
Aspergillus japonicus
endo-β-1,4-xylanase
enzymes
laccase
β-xylosidase
title_short Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
title_full Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
title_fullStr Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
title_full_unstemmed Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
title_sort Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
author Ferreira,Flaviane Lopes
author_facet Ferreira,Flaviane Lopes
Dall'Antonia,Cesar Barretta
Shiga,Emerson Andrade
Alvim,Larissa Juliani
Pessoni,Rosemeire Aparecida Bom
author_role author
author2 Dall'Antonia,Cesar Barretta
Shiga,Emerson Andrade
Alvim,Larissa Juliani
Pessoni,Rosemeire Aparecida Bom
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Flaviane Lopes
Dall'Antonia,Cesar Barretta
Shiga,Emerson Andrade
Alvim,Larissa Juliani
Pessoni,Rosemeire Aparecida Bom
dc.subject.por.fl_str_mv anamorphic fungi
Aspergillus japonicus
endo-β-1,4-xylanase
enzymes
laccase
β-xylosidase
topic anamorphic fungi
Aspergillus japonicus
endo-β-1,4-xylanase
enzymes
laccase
β-xylosidase
description ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/2236-8906-40/2017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
publisher.none.fl_str_mv Instituto de Pesquisas Ambientais
dc.source.none.fl_str_mv Hoehnea v.45 n.1 2018
reponame:Hoehnea
instname:Instituto de Botânica (IBT)
instacron:IBT
instname_str Instituto de Botânica (IBT)
instacron_str IBT
institution IBT
reponame_str Hoehnea
collection Hoehnea
repository.name.fl_str_mv Hoehnea - Instituto de Botânica (IBT)
repository.mail.fl_str_mv ||hoehneaibt@gmail.com
_version_ 1754193647894528000