Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Hoehnea |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134 |
Resumo: | ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon. |
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Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1anamorphic fungiAspergillus japonicusendo-β-1,4-xylanaseenzymeslaccaseβ-xylosidaseABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon.Instituto de Pesquisas Ambientais2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134Hoehnea v.45 n.1 2018reponame:Hoehneainstname:Instituto de Botânica (IBT)instacron:IBT10.1590/2236-8906-40/2017info:eu-repo/semantics/openAccessFerreira,Flaviane LopesDall'Antonia,Cesar BarrettaShiga,Emerson AndradeAlvim,Larissa JulianiPessoni,Rosemeire Aparecida Bomeng2018-04-23T00:00:00Zoai:scielo:S2236-89062018000100134Revistahttp://www.ibot.sp.gov.br/publicacoes/hoehnea/sobre_hoehnea.phpPUBhttps://old.scielo.br/oai/scielo-oai.php||hoehneaibt@gmail.com2236-89060073-2877opendoar:2018-04-23T00:00Hoehnea - Instituto de Botânica (IBT)false |
dc.title.none.fl_str_mv |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
title |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
spellingShingle |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 Ferreira,Flaviane Lopes anamorphic fungi Aspergillus japonicus endo-β-1,4-xylanase enzymes laccase β-xylosidase |
title_short |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
title_full |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
title_fullStr |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
title_full_unstemmed |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
title_sort |
Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi1 |
author |
Ferreira,Flaviane Lopes |
author_facet |
Ferreira,Flaviane Lopes Dall'Antonia,Cesar Barretta Shiga,Emerson Andrade Alvim,Larissa Juliani Pessoni,Rosemeire Aparecida Bom |
author_role |
author |
author2 |
Dall'Antonia,Cesar Barretta Shiga,Emerson Andrade Alvim,Larissa Juliani Pessoni,Rosemeire Aparecida Bom |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Flaviane Lopes Dall'Antonia,Cesar Barretta Shiga,Emerson Andrade Alvim,Larissa Juliani Pessoni,Rosemeire Aparecida Bom |
dc.subject.por.fl_str_mv |
anamorphic fungi Aspergillus japonicus endo-β-1,4-xylanase enzymes laccase β-xylosidase |
topic |
anamorphic fungi Aspergillus japonicus endo-β-1,4-xylanase enzymes laccase β-xylosidase |
description |
ABSTRACT The aim of the present work was to assess the enzymatic activity of six strains of filamentous fungi grown in liquid media containing 1% sugarcane bagasse as the sole carbon source. All fungal strains were able to use this agro-industrial residue, producing various types of enzymes, such as cellulases, xylanases, amylases, pectinases, and laccases. However, Aspergillus japonicus Saito was the most efficient producer, showing the highest enzymatic activity for laccase (395.73 U L-1), endo-β-1,4-xylanase (3.55 U mL-1) and β-xylosidase (9.74 U mL-1) at seven, fourteen and twenty-one days in culture, respectively. Furthermore, the endo-β-1,4-xylanases and β-xylosidases of A. japonicus showed maximum activity at 50°C, and pH 5.5 and pH 3.5-4.5, respectively. Thus, these results indicate that A. japonicus has a great biotechnological potential for the production of these enzymes using sugarcane bagasse as the sole source of carbon. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2236-89062018000100134 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2236-8906-40/2017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais |
publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais |
dc.source.none.fl_str_mv |
Hoehnea v.45 n.1 2018 reponame:Hoehnea instname:Instituto de Botânica (IBT) instacron:IBT |
instname_str |
Instituto de Botânica (IBT) |
instacron_str |
IBT |
institution |
IBT |
reponame_str |
Hoehnea |
collection |
Hoehnea |
repository.name.fl_str_mv |
Hoehnea - Instituto de Botânica (IBT) |
repository.mail.fl_str_mv |
||hoehneaibt@gmail.com |
_version_ |
1754193647894528000 |