Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine

Detalhes bibliográficos
Autor(a) principal: Guerra, Sylvia de Fátima dos Santos
Data de Publicação: 2019
Outros Autores: Fecury, Priscylla C. M. S, Bezerra, Delana Andreza Melo, Lobo, Patricia S., Penha Júnior, Edvaldo Tavares da, Sousa Júnior, Edivaldo Costa, Mascarenhas, Joana D'Arc Pereira, Soares, Luana da Silva, Justino, Maria Cleonice Aguiar, Linhares, Alexandre da Costa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3758
Resumo: Species A rotavirus still remains a major cause of acute gastroenteritis in infants and young children. Globally, six genotypes (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]) account for >90% of circulating strains; however, genotype G12 in combination with P[6] or P[9] has been detected at increasing rates. We sought to broaden our knowledge about the rotavirus strains circulating during the early post-vaccine-introduction period. Stool samples were obtained from children hospitalised for acute gastroenteritis in Belém, Northern Brazil, from May 2008 to May 2011 and examined by reverse transcription polymerase chain reaction and nucleotide sequencing. A total of 122 out of the original 1076 rotavirus strains were judged to be non-typeable in the first analysis and were therefore re-examined. G2P[4] was the most prevalent genotype (58.0%), followed by G1P[8] (16.9%), and G12P[6] (7.5%). G12P[6] strains were identified at similar rates during the first (2.5%) and second (3.9%) years, and the rate jumped to 15.6% in the third year. Analysis of VP7 sequences of the G12P[6] strains showed that they belonged to lineage III. In addition, co-circulating G12P[6] strains displaying long and short RNA patterns were found to belong to the Wa-like and DS-1-like constellation, respectively. Additional unusual circulating strains G12P[9] and G3P[9] were also identified. This hospital-based study showed a high prevalence of G12P[6] strains in the third year of surveillance. Our results highlight the need for continuous longitudinal monitoring of circulating rotavirus strains after introduction of rotavirus vaccines in Brazil and elsewhere.
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spelling Guerra, Sylvia de Fátima dos SantosFecury, Priscylla C. M. SBezerra, Delana Andreza MeloLobo, Patricia S.Penha Júnior, Edvaldo Tavares daSousa Júnior, Edivaldo CostaMascarenhas, Joana D'Arc PereiraSoares, Luana da SilvaJustino, Maria Cleonice AguiarLinhares, Alexandre da Costa2019-06-18T18:22:05Z2019-06-18T18:22:05Z2019GUERRA, Sylvia de Fátima dos Santos et al. Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine. Archives of Virology, v. 164, n. 8, p. 2107-2117, Aug. 2019. DOI: https://doi.org/10.1007/s00705-019-04295-w1432-8798https://patua.iec.gov.br/handle/iec/375810.1007/s00705-019-04295-wSpecies A rotavirus still remains a major cause of acute gastroenteritis in infants and young children. Globally, six genotypes (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]) account for >90% of circulating strains; however, genotype G12 in combination with P[6] or P[9] has been detected at increasing rates. We sought to broaden our knowledge about the rotavirus strains circulating during the early post-vaccine-introduction period. Stool samples were obtained from children hospitalised for acute gastroenteritis in Belém, Northern Brazil, from May 2008 to May 2011 and examined by reverse transcription polymerase chain reaction and nucleotide sequencing. A total of 122 out of the original 1076 rotavirus strains were judged to be non-typeable in the first analysis and were therefore re-examined. G2P[4] was the most prevalent genotype (58.0%), followed by G1P[8] (16.9%), and G12P[6] (7.5%). G12P[6] strains were identified at similar rates during the first (2.5%) and second (3.9%) years, and the rate jumped to 15.6% in the third year. Analysis of VP7 sequences of the G12P[6] strains showed that they belonged to lineage III. In addition, co-circulating G12P[6] strains displaying long and short RNA patterns were found to belong to the Wa-like and DS-1-like constellation, respectively. Additional unusual circulating strains G12P[9] and G3P[9] were also identified. This hospital-based study showed a high prevalence of G12P[6] strains in the third year of surveillance. Our results highlight the need for continuous longitudinal monitoring of circulating rotavirus strains after introduction of rotavirus vaccines in Brazil and elsewhere.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engSpringer VerlagEmergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInfecções por Rotavirus / virologiaVacinas contra Rotavirus / uso terapêuticoGastroenterite / virologiaReação em Cadeia da Polimerase / métodosMonitoramento Epidemiológicoinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALEmergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine.pdfEmergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/e2aea38d-d8bb-41cc-aeb4-58bfe2cd366e/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
title Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
spellingShingle Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
Guerra, Sylvia de Fátima dos Santos
Infecções por Rotavirus / virologia
Vacinas contra Rotavirus / uso terapêutico
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Monitoramento Epidemiológico
title_short Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
title_full Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
title_fullStr Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
title_full_unstemmed Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
title_sort Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine
author Guerra, Sylvia de Fátima dos Santos
author_facet Guerra, Sylvia de Fátima dos Santos
Fecury, Priscylla C. M. S
Bezerra, Delana Andreza Melo
Lobo, Patricia S.
Penha Júnior, Edvaldo Tavares da
Sousa Júnior, Edivaldo Costa
Mascarenhas, Joana D'Arc Pereira
Soares, Luana da Silva
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
author_role author
author2 Fecury, Priscylla C. M. S
Bezerra, Delana Andreza Melo
Lobo, Patricia S.
Penha Júnior, Edvaldo Tavares da
Sousa Júnior, Edivaldo Costa
Mascarenhas, Joana D'Arc Pereira
Soares, Luana da Silva
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guerra, Sylvia de Fátima dos Santos
Fecury, Priscylla C. M. S
Bezerra, Delana Andreza Melo
Lobo, Patricia S.
Penha Júnior, Edvaldo Tavares da
Sousa Júnior, Edivaldo Costa
Mascarenhas, Joana D'Arc Pereira
Soares, Luana da Silva
Justino, Maria Cleonice Aguiar
Linhares, Alexandre da Costa
dc.subject.decsPrimary.pt_BR.fl_str_mv Infecções por Rotavirus / virologia
Vacinas contra Rotavirus / uso terapêutico
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Monitoramento Epidemiológico
topic Infecções por Rotavirus / virologia
Vacinas contra Rotavirus / uso terapêutico
Gastroenterite / virologia
Reação em Cadeia da Polimerase / métodos
Monitoramento Epidemiológico
description Species A rotavirus still remains a major cause of acute gastroenteritis in infants and young children. Globally, six genotypes (G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]) account for >90% of circulating strains; however, genotype G12 in combination with P[6] or P[9] has been detected at increasing rates. We sought to broaden our knowledge about the rotavirus strains circulating during the early post-vaccine-introduction period. Stool samples were obtained from children hospitalised for acute gastroenteritis in Belém, Northern Brazil, from May 2008 to May 2011 and examined by reverse transcription polymerase chain reaction and nucleotide sequencing. A total of 122 out of the original 1076 rotavirus strains were judged to be non-typeable in the first analysis and were therefore re-examined. G2P[4] was the most prevalent genotype (58.0%), followed by G1P[8] (16.9%), and G12P[6] (7.5%). G12P[6] strains were identified at similar rates during the first (2.5%) and second (3.9%) years, and the rate jumped to 15.6% in the third year. Analysis of VP7 sequences of the G12P[6] strains showed that they belonged to lineage III. In addition, co-circulating G12P[6] strains displaying long and short RNA patterns were found to belong to the Wa-like and DS-1-like constellation, respectively. Additional unusual circulating strains G12P[9] and G3P[9] were also identified. This hospital-based study showed a high prevalence of G12P[6] strains in the third year of surveillance. Our results highlight the need for continuous longitudinal monitoring of circulating rotavirus strains after introduction of rotavirus vaccines in Brazil and elsewhere.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-06-18T18:22:05Z
dc.date.available.fl_str_mv 2019-06-18T18:22:05Z
dc.date.issued.fl_str_mv 2019
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dc.identifier.citation.fl_str_mv GUERRA, Sylvia de Fátima dos Santos et al. Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine. Archives of Virology, v. 164, n. 8, p. 2107-2117, Aug. 2019. DOI: https://doi.org/10.1007/s00705-019-04295-w
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3758
dc.identifier.issn.-.fl_str_mv 1432-8798
dc.identifier.doi.-.fl_str_mv 10.1007/s00705-019-04295-w
identifier_str_mv GUERRA, Sylvia de Fátima dos Santos et al. Emergence of G12P[6] rotavirus strains among hospitalised children with acute gastroenteritis in Belém, Northern Brazil, following introduction of a rotavirus vaccine. Archives of Virology, v. 164, n. 8, p. 2107-2117, Aug. 2019. DOI: https://doi.org/10.1007/s00705-019-04295-w
1432-8798
10.1007/s00705-019-04295-w
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