Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3166 |
Resumo: | The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete anti-saliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response. |
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Batista, Luís F. SUtsunomiya, Yuri TSilva, Thaís B. FCarneiro, Mariana MPaiva, Joyr S. FSilva, Rafaela BTomokane, Thaíse YRossi, Claudio NPacheco, Acácio DTorrecilha, Rafaela B. PSilveira, Fernando TobiasMarcondes, MaryNunes, Cáris MLaurenti, Márcia Dalastra2018-06-14T11:11:46Z2018-06-14T11:11:46Z2018BATISTA, Luís F. S. et al. Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva. PLoS ONE, v. 13, n. 5, e0197215, May 2018.1932-6203https://patua.iec.gov.br/handle/iec/316610.1371/journal.pone.0197215The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete anti-saliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response.Fundação de Amparo à Pesquisa do Estado de São Paulo, Brazil (FAPESP) grants no. 2012/50285-9 (M.D.L.), no. 2012/05847-9 (L.F.S.B.), and no. 2014/01095-8 (Y.T.U.); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil grant no. 476479/2012-6 (M.D.L.); and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes).Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Patologia Veterinária. São Paulo, SP, Brazil / Universidade Salvador. Escola de Saúde. Salvador, BA, Brazil.Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal. Jaboticabal, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Doenças Infecciosas. São Paulo, SP, Brazil.Universidade Salvador. Escola de Saúde. Salvador, BA, Brazil.Universidade Salvador. Escola de Saúde. Salvador, BA, Brazil.Universidade Salvador. Escola de Saúde. Salvador, BA, Brazil.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Doenças Infecciosas. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departmento de Clínica. São Paulo, SP, Brazil.Universidade Estadual Paulista. Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brazil.Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Medicina Veterinária Preventiva e Reprodução Animal. Jaboticabal, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Estadual Paulista. Faculdade de Medicina Veterinária. Departamento de Clínica, Cirurgia e Reprodução Animal. Araçatuba, SP, Brazil.Universidade Estadual Paulista. Faculdade de Medicina Veterinária. Departmento de Saúde Animal e Produção. Araçatuba, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Laboratório de Patologia de Doenças Infecciosas. São Paulo, SP, Brazil.engPublic Library of ScienceCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis salivainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniose / parasitologiaLeishmania infantum / parasitologiaCães / lesõesSaliva / secreçãoImunoglobulina G / genéticaLeishmaniose caninainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdfCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdfapplication/pdf2157979https://patua.iec.gov.br/bitstreams/23d6901b-3e22-41a9-9130-74690f00bf6a/download9956680d87d53dc737cdf4eb06f651ecMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/b319722f-0839-4de6-b387-fbd30cd39227/download52f1732ea66fbd1123abe39f5373b797MD52TEXTCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdf.txtCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdf.txtExtracted texttext/plain53315https://patua.iec.gov.br/bitstreams/f50c9051-2967-4175-92cf-f88979dc8d97/downloadbf10fa9891c908f757e65ac5e90b9c3bMD55THUMBNAILCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdf.jpgCanine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva.pdf.jpgGenerated Thumbnailimage/jpeg6162https://patua.iec.gov.br/bitstreams/bd85dcf1-e1e0-4273-abe1-13668bc2251a/download77149165bf9f61469b25b516aa732d56MD56iec/31662023-03-10 13:04:54.45oai:patua.iec.gov.br:iec/3166https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2023-03-10T13:04:54Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
spellingShingle |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva Batista, Luís F. S Leishmaniose / parasitologia Leishmania infantum / parasitologia Cães / lesões Saliva / secreção Imunoglobulina G / genética Leishmaniose canina |
title_short |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_full |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_fullStr |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_full_unstemmed |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_sort |
Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
author |
Batista, Luís F. S |
author_facet |
Batista, Luís F. S Utsunomiya, Yuri T Silva, Thaís B. F Carneiro, Mariana M Paiva, Joyr S. F Silva, Rafaela B Tomokane, Thaíse Y Rossi, Claudio N Pacheco, Acácio D Torrecilha, Rafaela B. P Silveira, Fernando Tobias Marcondes, Mary Nunes, Cáris M Laurenti, Márcia Dalastra |
author_role |
author |
author2 |
Utsunomiya, Yuri T Silva, Thaís B. F Carneiro, Mariana M Paiva, Joyr S. F Silva, Rafaela B Tomokane, Thaíse Y Rossi, Claudio N Pacheco, Acácio D Torrecilha, Rafaela B. P Silveira, Fernando Tobias Marcondes, Mary Nunes, Cáris M Laurenti, Márcia Dalastra |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Batista, Luís F. S Utsunomiya, Yuri T Silva, Thaís B. F Carneiro, Mariana M Paiva, Joyr S. F Silva, Rafaela B Tomokane, Thaíse Y Rossi, Claudio N Pacheco, Acácio D Torrecilha, Rafaela B. P Silveira, Fernando Tobias Marcondes, Mary Nunes, Cáris M Laurenti, Márcia Dalastra |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmaniose / parasitologia Leishmania infantum / parasitologia Cães / lesões Saliva / secreção Imunoglobulina G / genética Leishmaniose canina |
topic |
Leishmaniose / parasitologia Leishmania infantum / parasitologia Cães / lesões Saliva / secreção Imunoglobulina G / genética Leishmaniose canina |
description |
The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete anti-saliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-06-14T11:11:46Z |
dc.date.available.fl_str_mv |
2018-06-14T11:11:46Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BATISTA, Luís F. S. et al. Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva. PLoS ONE, v. 13, n. 5, e0197215, May 2018. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3166 |
dc.identifier.issn.-.fl_str_mv |
1932-6203 |
dc.identifier.doi.-.fl_str_mv |
10.1371/journal.pone.0197215 |
identifier_str_mv |
BATISTA, Luís F. S. et al. Canine leishmaniasis: genome-wide analysis and antibody response to Lutzomyia longipalpis saliva. PLoS ONE, v. 13, n. 5, e0197215, May 2018. 1932-6203 10.1371/journal.pone.0197215 |
url |
https://patua.iec.gov.br/handle/iec/3166 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
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