Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/2890 |
Resumo: | Plasmodium vivax is the most widely distributed malaria species and the most prevalent species of malaria in America and Asia. Vaccine development against P. vivax is considered a priority in the global program for the eradication of malaria. Earlier studies have characterized the Apical Membrane Antigen 1 (AMA-1) ectodomain and the C-terminal region (19 kDa) of the Merozoite Surface Protein 1 (MSP-1) of P. vivax as immunodominant antigens. Based on this characterization, we designed a chimeric recombinant protein containing both merozoite immunodominant domains (PvAMA1(66)-MSP1(19)). The recombinant PvAMA166-MSP119 was successfully expressed in Pichia pastoris and used to immunize two different mouse strains (BALB/c and C57BL/6) in the presence of the Poly (I:C) as an adjuvant. Immunization with the chimeric protein induced high antibody titers against both proteins in both strains of mice as detected by ELISA. Antisera also recognized the native proteins expressed on the merozoites of mature P. vivax schizonts. Moreover, this antigen was able to induce IFN-gamma-secreting cells in C57BL/6 mice. These findings indicate that this novel yeast recombinant protein containing PvAMA1(66) and PvMSP1(19) is advantageous, because of improved antibody titers and cellular immune response. Therefore, this formulation should be further developed for pre-clinical trials in non-human primates as a potential candidate for a P. vivax vaccine. |
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Rocha, Mariana VilelaFrancoso, Katia SanchesLima, Luciana ChagasCamargo, Tarsila MendesMachado, Ricardo Luis DantasCosta, Fabio T. MRenia, LaurentNosten, FrancoisRussell, BruceRodrigues, Mauricio MSoares, Irene S2017-11-28T18:28:31Z2017-11-28T18:28:31Z2017ROCHA, Mariana Vilela et al. Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19). Vaccine, v. 35, n. 18, p. 2463-2472, Apr. 2017.0264-410Xhttps://patua.iec.gov.br/handle/iec/289010.1016/j.vaccine.2017.03.023Plasmodium vivax is the most widely distributed malaria species and the most prevalent species of malaria in America and Asia. Vaccine development against P. vivax is considered a priority in the global program for the eradication of malaria. Earlier studies have characterized the Apical Membrane Antigen 1 (AMA-1) ectodomain and the C-terminal region (19 kDa) of the Merozoite Surface Protein 1 (MSP-1) of P. vivax as immunodominant antigens. Based on this characterization, we designed a chimeric recombinant protein containing both merozoite immunodominant domains (PvAMA1(66)-MSP1(19)). The recombinant PvAMA166-MSP119 was successfully expressed in Pichia pastoris and used to immunize two different mouse strains (BALB/c and C57BL/6) in the presence of the Poly (I:C) as an adjuvant. Immunization with the chimeric protein induced high antibody titers against both proteins in both strains of mice as detected by ELISA. Antisera also recognized the native proteins expressed on the merozoites of mature P. vivax schizonts. Moreover, this antigen was able to induce IFN-gamma-secreting cells in C57BL/6 mice. These findings indicate that this novel yeast recombinant protein containing PvAMA1(66) and PvMSP1(19) is advantageous, because of improved antibody titers and cellular immune response. Therefore, this formulation should be further developed for pre-clinical trials in non-human primates as a potential candidate for a P. vivax vaccine.This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 475500/2012-1), Instituto Nacional de Ciência e Tecnologia de Vacinas (INCTV), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2012/13032-5). MVR was sponsored by FAPESP fellowships (2011/23278-9 and 2013/01487-0). RLDM, FTMC and ISS are recipients of fellowships from CNPq.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Genética, Evolução e Bioagentes. Laboratório de Doenças Tropicais. Campinas, SP, Brazil.Biopolis, Agency for Science Technology and Research. Singapore Immunology Network. Singapore, SG.Mahidol University, Mae Sot. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit. Shoklo Malaria Research Unit. Thailand, TH / University of Oxford Old Road Campus. Department of Medicine Research Building. Centre for Tropical Medicine and Global Health, Nuffield. Oxford, UK.Agency for Science Technology and Research. Biopolis. Singapore Immunology Network. Singapore, SG / University of Otago. Department of Microbiology and Immunology. Dunedin, NZ, New Zealand.Universidade Federal de São Paulo. Escola Paulista de Medicina. Centro de Terapia Celular e Molecular. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brazil.engElsevierGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMaláriaPlasmodium vivaxVacinasVacinas de DNAEpitopos ImunodominantesVacinas Atenuadas / uso terapêuticoinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-871https://patua.iec.gov.br/bitstreams/51f25acb-0154-4bfb-9c35-0b923b85521c/download52f1732ea66fbd1123abe39f5373b797MD52ORIGINALGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdfGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdfapplication/pdf189983https://patua.iec.gov.br/bitstreams/eb0ea72b-ae9a-4d8b-83ea-5fb7e3c204f8/download70c9bc1f4ee8fdcf112f916003bac471MD53TEXTGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdf.txtGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdf.txtExtracted texttext/plain5082https://patua.iec.gov.br/bitstreams/75f98324-45d4-4e5c-a4ae-de8bf943fcdf/download05197cd49408933f80365599a69c6015MD56THUMBNAILGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdf.jpgGeneration, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19).pdf.jpgGenerated Thumbnailimage/jpeg5790https://patua.iec.gov.br/bitstreams/96868dd4-8fa9-45d6-a6cc-106d5653d952/downloadeeb15c054a2dc71517b6d8781ddbd233MD57iec/28902022-10-20 23:31:29.916oai:patua.iec.gov.br:iec/2890https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:31:29Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)falseVG9kb3Mgb3MgZG9jdW1lbnRvcyBkZXNzYSBjb2xlw6fDo28gc2VndWVtIGEgTGljZW7Dp2EgQ3JlYXRpdmUgY29tbW9ucy4= |
dc.title.pt_BR.fl_str_mv |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
title |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
spellingShingle |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) Rocha, Mariana Vilela Malária Plasmodium vivax Vacinas Vacinas de DNA Epitopos Imunodominantes Vacinas Atenuadas / uso terapêutico |
title_short |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
title_full |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
title_fullStr |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
title_full_unstemmed |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
title_sort |
Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19) |
author |
Rocha, Mariana Vilela |
author_facet |
Rocha, Mariana Vilela Francoso, Katia Sanches Lima, Luciana Chagas Camargo, Tarsila Mendes Machado, Ricardo Luis Dantas Costa, Fabio T. M Renia, Laurent Nosten, Francois Russell, Bruce Rodrigues, Mauricio M Soares, Irene S |
author_role |
author |
author2 |
Francoso, Katia Sanches Lima, Luciana Chagas Camargo, Tarsila Mendes Machado, Ricardo Luis Dantas Costa, Fabio T. M Renia, Laurent Nosten, Francois Russell, Bruce Rodrigues, Mauricio M Soares, Irene S |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rocha, Mariana Vilela Francoso, Katia Sanches Lima, Luciana Chagas Camargo, Tarsila Mendes Machado, Ricardo Luis Dantas Costa, Fabio T. M Renia, Laurent Nosten, Francois Russell, Bruce Rodrigues, Mauricio M Soares, Irene S |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Malária Plasmodium vivax Vacinas Vacinas de DNA Epitopos Imunodominantes Vacinas Atenuadas / uso terapêutico |
topic |
Malária Plasmodium vivax Vacinas Vacinas de DNA Epitopos Imunodominantes Vacinas Atenuadas / uso terapêutico |
description |
Plasmodium vivax is the most widely distributed malaria species and the most prevalent species of malaria in America and Asia. Vaccine development against P. vivax is considered a priority in the global program for the eradication of malaria. Earlier studies have characterized the Apical Membrane Antigen 1 (AMA-1) ectodomain and the C-terminal region (19 kDa) of the Merozoite Surface Protein 1 (MSP-1) of P. vivax as immunodominant antigens. Based on this characterization, we designed a chimeric recombinant protein containing both merozoite immunodominant domains (PvAMA1(66)-MSP1(19)). The recombinant PvAMA166-MSP119 was successfully expressed in Pichia pastoris and used to immunize two different mouse strains (BALB/c and C57BL/6) in the presence of the Poly (I:C) as an adjuvant. Immunization with the chimeric protein induced high antibody titers against both proteins in both strains of mice as detected by ELISA. Antisera also recognized the native proteins expressed on the merozoites of mature P. vivax schizonts. Moreover, this antigen was able to induce IFN-gamma-secreting cells in C57BL/6 mice. These findings indicate that this novel yeast recombinant protein containing PvAMA1(66) and PvMSP1(19) is advantageous, because of improved antibody titers and cellular immune response. Therefore, this formulation should be further developed for pre-clinical trials in non-human primates as a potential candidate for a P. vivax vaccine. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-11-28T18:28:31Z |
dc.date.available.fl_str_mv |
2017-11-28T18:28:31Z |
dc.date.issued.fl_str_mv |
2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ROCHA, Mariana Vilela et al. Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19). Vaccine, v. 35, n. 18, p. 2463-2472, Apr. 2017. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/2890 |
dc.identifier.issn.-.fl_str_mv |
0264-410X |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.vaccine.2017.03.023 |
identifier_str_mv |
ROCHA, Mariana Vilela et al. Generation, characterization and immunogenicity of a novel chimeric recombinant protein based on Plasmodium vivax AMA-1 and MSP1(19). Vaccine, v. 35, n. 18, p. 2463-2472, Apr. 2017. 0264-410X 10.1016/j.vaccine.2017.03.023 |
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Elsevier |
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Elsevier |
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