Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4601 |
Resumo: | Present work aimed to identify blood feeding sources and attempt to detect Leishmania DNA in Nyssomyia antunesi, suspected vector of Leishmania sp., from a park in the urban center of Belém, the capital of Pará State, in the Brazilian Amazon. Entire bodies and gut contents of Ny. antunesi engorged females, previously captured in the urban park with Centers for Disease Control (CDC) light traps and aspiration on tree bases, were subjected to Leishmania and vertebrate DNA detection through amplification of the Leishmania mini-exon and vertebrate cytochrome b (cyt b) gene regions, respectively. The quality of DNA extraction from entire bodies was ensured through amplification of the dipteran cyt b region. The vertebrate cyt b amplicons were sequenced and compared with those available on GenBank. A maximum likelihood phylogenetic tree was constructed to assess the clustering patterns of these sequences. Leishmania DNA was not detected. The sequences of 13 vertebrate cyt b amplicons were considered informative, exhibiting similarity and clustering with the following six vertebrate species: Dasyprocta leporina (1), Cuniculus paca (1), Tamandua tetradactyla (4), Choloepus didactylus (4), Pteroglossus aracari aracari (2), Homo sapiens (1). The samples of D. leporina and C. paca were obtained from the CDC canopy, whereas the others were by aspiration from tree bases. The present results revealed the eclectic and opportunist blood-feeding behavior of Ny. antunesi, with birds and mammals, these last ones acting as potential reservoirs for Leishmania species, distributed throughout the vertical forest strata. |
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Pimentel, Amanda CostaUzcátegui, Yetsenia Del Valle SánchezLima, Ana Carolina Stocco deSilveira, Fernando TobiasSantos, Thiago Vasconcelos dosIshikawa, Edna Aoba Yassui2022-08-16T16:11:47Z2022-08-16T16:11:47Z2022PIMENTEL, Amanda Costa et al. Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon. Journal of Medical Entomology, v. xx, n. xx, p. 1-6. 2022. DOI: https://doi.org/10.1093/jme/tjac108. Disponível em: https://academic.oup.com/jme/advance-article/doi/10.1093/jme/tjac108/6651047.1938-2928https://patua.iec.gov.br/handle/iec/460110.1093/jme/tjac108Present work aimed to identify blood feeding sources and attempt to detect Leishmania DNA in Nyssomyia antunesi, suspected vector of Leishmania sp., from a park in the urban center of Belém, the capital of Pará State, in the Brazilian Amazon. Entire bodies and gut contents of Ny. antunesi engorged females, previously captured in the urban park with Centers for Disease Control (CDC) light traps and aspiration on tree bases, were subjected to Leishmania and vertebrate DNA detection through amplification of the Leishmania mini-exon and vertebrate cytochrome b (cyt b) gene regions, respectively. The quality of DNA extraction from entire bodies was ensured through amplification of the dipteran cyt b region. The vertebrate cyt b amplicons were sequenced and compared with those available on GenBank. A maximum likelihood phylogenetic tree was constructed to assess the clustering patterns of these sequences. Leishmania DNA was not detected. The sequences of 13 vertebrate cyt b amplicons were considered informative, exhibiting similarity and clustering with the following six vertebrate species: Dasyprocta leporina (1), Cuniculus paca (1), Tamandua tetradactyla (4), Choloepus didactylus (4), Pteroglossus aracari aracari (2), Homo sapiens (1). The samples of D. leporina and C. paca were obtained from the CDC canopy, whereas the others were by aspiration from tree bases. The present results revealed the eclectic and opportunist blood-feeding behavior of Ny. antunesi, with birds and mammals, these last ones acting as potential reservoirs for Leishmania species, distributed throughout the vertical forest strata.Universidade Federal do Pará and Instituto Evandro Chagas/ Ministry of Health, Brazil / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brasil’ (CAPES) - Financial code 001Universidade Federal do Pará. Núcleo de Medicina Tropical. Programa de Pós Graduação em Doenças Tropicais. Belém, PA, Brazil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidad de Los Andes. Facultad de Ciencias. Departamento de Biología. Mérida, Venezuela.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Pará. Núcleo de Medicina Tropical. Programa de Pós Graduação em Doenças Tropicais. Belém, PA, Brazil / Universidad de Los Andes. Facultad de Ciencias. Departamento de Biología. Mérida, Venezuela.Universidade Federal do Pará. Instituto de Ciências Biológicas. Programa de Pós Graduação em Biologia de Agentes Infecciosos e Parasitários. Belém, PA, Brazil / Universidad de Los Andes. Facultad de Ciencias. Departamento de Biología. Mérida, Venezuela.Universidade Federal do Pará. Núcleo de Medicina Tropical. Programa de Pós Graduação em Doenças Tropicais. Belém, PA, Brazil.engAcesso AbertoOxford University PressBlood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhlebotomus / anatomia & histologiaSangue / parasitologiaLeishmania / parasitologiaVetores de Doençasreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECinfo:eu-repo/semantics/openAccessORIGINALBlood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon.pdfBlood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon.pdfapplication/pdf716146https://patua.iec.gov.br/bitstreams/7cb23724-13e7-4eb8-b3f4-cb27e0291b0c/downloadfd8665c555f0549b72b1b6a17d56a904MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
title |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
spellingShingle |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon Pimentel, Amanda Costa Phlebotomus / anatomia & histologia Sangue / parasitologia Leishmania / parasitologia Vetores de Doenças |
title_short |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
title_full |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
title_fullStr |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
title_full_unstemmed |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
title_sort |
Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon |
author |
Pimentel, Amanda Costa |
author_facet |
Pimentel, Amanda Costa Uzcátegui, Yetsenia Del Valle Sánchez Lima, Ana Carolina Stocco de Silveira, Fernando Tobias Santos, Thiago Vasconcelos dos Ishikawa, Edna Aoba Yassui |
author_role |
author |
author2 |
Uzcátegui, Yetsenia Del Valle Sánchez Lima, Ana Carolina Stocco de Silveira, Fernando Tobias Santos, Thiago Vasconcelos dos Ishikawa, Edna Aoba Yassui |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pimentel, Amanda Costa Uzcátegui, Yetsenia Del Valle Sánchez Lima, Ana Carolina Stocco de Silveira, Fernando Tobias Santos, Thiago Vasconcelos dos Ishikawa, Edna Aoba Yassui |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Phlebotomus / anatomia & histologia Sangue / parasitologia Leishmania / parasitologia Vetores de Doenças |
topic |
Phlebotomus / anatomia & histologia Sangue / parasitologia Leishmania / parasitologia Vetores de Doenças |
description |
Present work aimed to identify blood feeding sources and attempt to detect Leishmania DNA in Nyssomyia antunesi, suspected vector of Leishmania sp., from a park in the urban center of Belém, the capital of Pará State, in the Brazilian Amazon. Entire bodies and gut contents of Ny. antunesi engorged females, previously captured in the urban park with Centers for Disease Control (CDC) light traps and aspiration on tree bases, were subjected to Leishmania and vertebrate DNA detection through amplification of the Leishmania mini-exon and vertebrate cytochrome b (cyt b) gene regions, respectively. The quality of DNA extraction from entire bodies was ensured through amplification of the dipteran cyt b region. The vertebrate cyt b amplicons were sequenced and compared with those available on GenBank. A maximum likelihood phylogenetic tree was constructed to assess the clustering patterns of these sequences. Leishmania DNA was not detected. The sequences of 13 vertebrate cyt b amplicons were considered informative, exhibiting similarity and clustering with the following six vertebrate species: Dasyprocta leporina (1), Cuniculus paca (1), Tamandua tetradactyla (4), Choloepus didactylus (4), Pteroglossus aracari aracari (2), Homo sapiens (1). The samples of D. leporina and C. paca were obtained from the CDC canopy, whereas the others were by aspiration from tree bases. The present results revealed the eclectic and opportunist blood-feeding behavior of Ny. antunesi, with birds and mammals, these last ones acting as potential reservoirs for Leishmania species, distributed throughout the vertical forest strata. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-08-16T16:11:47Z |
dc.date.available.fl_str_mv |
2022-08-16T16:11:47Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PIMENTEL, Amanda Costa et al. Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon. Journal of Medical Entomology, v. xx, n. xx, p. 1-6. 2022. DOI: https://doi.org/10.1093/jme/tjac108. Disponível em: https://academic.oup.com/jme/advance-article/doi/10.1093/jme/tjac108/6651047. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4601 |
dc.identifier.issn.pt_BR.fl_str_mv |
1938-2928 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1093/jme/tjac108 |
identifier_str_mv |
PIMENTEL, Amanda Costa et al. Blood feeding sources of Nyssomyia antunesi (Diptera: Psychodidae): a suspected vector of Leishmania (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon. Journal of Medical Entomology, v. xx, n. xx, p. 1-6. 2022. DOI: https://doi.org/10.1093/jme/tjac108. Disponível em: https://academic.oup.com/jme/advance-article/doi/10.1093/jme/tjac108/6651047. 1938-2928 10.1093/jme/tjac108 |
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https://patua.iec.gov.br/handle/iec/4601 |
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eng Acesso Aberto |
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eng |
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Oxford University Press |
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Oxford University Press |
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