Arboviruses in free-ranging birds and hematophagous arthropods (Diptera, Nematocera) from forest remnants and urbanized areas of an environmental protection area in the amazon biome

• Autor(a) principal: Ramos, Bruna Alves
• Data de Publicação: 2022
• Outros Autores: Chagas, Liliane Leal das, Silva, Franko de Arruda e, Santos, Eder Barros dos, Chiang, Jannifer Oliveira, Nunes Neto, Joaquim Pinto, Vieira, Durval Bertram Rodrigues, Rosa Junior, José Wilson, Silva, Eliana Vieira Pinto da, Freitas, Maria de Nazaré Oliveira, Santos, Maissa Maia, Pantoja, Jamilla Augusta de Sousa, Gonçalves, Ercília de Jesus, Barros, Landeson Junior Leopoldino, Silva, Sandro Patroca da, Aragão, Carine Fortes, Cruz, Ana Cecília Ribeiro, Casseb, Lívia Medeiros Neves, Souto, Lizandra Caroline dos Santos, Mascarenhas, Joana D'Arc Pereira, Furtado, Erilene Cristina da Silva, Silva, Raimundo Nelson Souza da, Casseb, Alexandre do Rosário, Martins, Lívia Caricio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
• Texto Completo: https://patua.iec.gov.br/handle/iec/4708
• topic: Arbovírus / isolamento & purificação; Infecções por Arbovirus / transmissão; Conservação dos Recursos Naturais; Urbanização; Aves; Artrópodes

The rapid and disorderly urbanization in the Amazon has resulted in the insertion of forest fragments into cities, causing the circulation of arboviruses, which can involve hematophagous arthropods and free-ranging birds in the transmission cycles in urban environments. This study aimed to evaluate the circulation of arboviruses in free ranging birds and hematophagous arthropods captured in an Environmental Protection Area in the Belem metropolitan area, Brazil. Birds were captured using mist nets, and hematophagous arthropods were collected using a human protected attraction technique and light traps. The birds’ sera were subjected to a hemagglutination inhibition test to detect antibodies against 29 arbovirus antigens. Arthropod macerates were inoculated into C6/36 and VERO cell cultures to attempt viral isolation and were tested using indirect immunofluorescence, subsequent genetic sequencing and submitted for phylogenetic analysis. Four bird sera were positive for arbovirus, and one batch of Psorophora ferox was positive for Flavivirus on viral isolation and indirect immunofluorescence. In addition, the Ilheus virus was detected in the sequencing and phylogenetic analysis. The presence of antibodies in sera from free-ranging birds and the isolation of Ilheus virus in Psorophora ferox indicate the circulation of arboviruses in forest remnants in the urban center of Belem.