Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3883 |
Resumo: | OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples. |
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Ribeiro, Ana Cláudia da SilvaCarvalho, Carlos Alberto Marques deCasseb, Samir Mansour MoraesRodrigues, Sueli GuerreiroVasconcelos, Pedro Fernando da CostaCarvalho, Valéria Lima2019-09-04T17:56:14Z2019-09-04T17:56:14Z2018RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019.2176-6223https://patua.iec.gov.br/handle/iec/388310.5123/s2176-62232018000400003OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples.This work was supported by grants from Ministério da Saúde, Brazil, Instituto Nacional de Ciência e Tecnologia de Febres Hemorrágicas Virais (grant no. 573739/2008-0), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant no. 457664/2013-4).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engMS/SVS/Instituto Evandro ChagasInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineagesPerfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleArbovirus / isolamento & purificaçãoAlphavirus / isolamento & purificaçãoVírus Chikungunya / isolamento & purificaçãoVírus MayaroLinhagem Celular / citologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages.pdfInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages.pdfapplication/pdf3677774https://patua.iec.gov.br/bitstreams/4d4c422d-d5f9-45c8-a6ef-f10eedb318c6/download507a412459e158d4756bcd5a71abd9b9MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
dc.title.alternative.pt_BR.fl_str_mv |
Perfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitos |
title |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
spellingShingle |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages Ribeiro, Ana Cláudia da Silva Arbovirus / isolamento & purificação Alphavirus / isolamento & purificação Vírus Chikungunya / isolamento & purificação Vírus Mayaro Linhagem Celular / citologia |
title_short |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
title_full |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
title_fullStr |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
title_full_unstemmed |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
title_sort |
Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages |
author |
Ribeiro, Ana Cláudia da Silva |
author_facet |
Ribeiro, Ana Cláudia da Silva Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Valéria Lima |
author_role |
author |
author2 |
Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Valéria Lima |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Ana Cláudia da Silva Carvalho, Carlos Alberto Marques de Casseb, Samir Mansour Moraes Rodrigues, Sueli Guerreiro Vasconcelos, Pedro Fernando da Costa Carvalho, Valéria Lima |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Arbovirus / isolamento & purificação Alphavirus / isolamento & purificação Vírus Chikungunya / isolamento & purificação Vírus Mayaro Linhagem Celular / citologia |
topic |
Arbovirus / isolamento & purificação Alphavirus / isolamento & purificação Vírus Chikungunya / isolamento & purificação Vírus Mayaro Linhagem Celular / citologia |
description |
OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-09-04T17:56:14Z |
dc.date.available.fl_str_mv |
2019-09-04T17:56:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3883 |
dc.identifier.issn.-.fl_str_mv |
2176-6223 |
dc.identifier.doi.-.fl_str_mv |
10.5123/s2176-62232018000400003 |
identifier_str_mv |
RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019. 2176-6223 10.5123/s2176-62232018000400003 |
url |
https://patua.iec.gov.br/handle/iec/3883 |
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eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MS/SVS/Instituto Evandro Chagas |
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MS/SVS/Instituto Evandro Chagas |
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