Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Ana Cláudia da Silva
Data de Publicação: 2018
Outros Autores: Carvalho, Carlos Alberto Marques de, Casseb, Samir Mansour Moraes, Rodrigues, Sueli Guerreiro, Vasconcelos, Pedro Fernando da Costa, Carvalho, Valéria Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3883
Resumo: OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples.
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spelling Ribeiro, Ana Cláudia da SilvaCarvalho, Carlos Alberto Marques deCasseb, Samir Mansour MoraesRodrigues, Sueli GuerreiroVasconcelos, Pedro Fernando da CostaCarvalho, Valéria Lima2019-09-04T17:56:14Z2019-09-04T17:56:14Z2018RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019.2176-6223https://patua.iec.gov.br/handle/iec/388310.5123/s2176-62232018000400003OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples.This work was supported by grants from Ministério da Saúde, Brazil, Instituto Nacional de Ciência e Tecnologia de Febres Hemorrágicas Virais (grant no. 573739/2008-0), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (grant no. 457664/2013-4).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.engMS/SVS/Instituto Evandro ChagasInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineagesPerfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleArbovirus / isolamento & purificaçãoAlphavirus / isolamento & purificaçãoVírus Chikungunya / isolamento & purificaçãoVírus MayaroLinhagem Celular / citologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages.pdfInfection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages.pdfapplication/pdf3677774https://patua.iec.gov.br/bitstreams/4d4c422d-d5f9-45c8-a6ef-f10eedb318c6/download507a412459e158d4756bcd5a71abd9b9MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
dc.title.alternative.pt_BR.fl_str_mv Perfis de infecção do vírus Mayaro e do vírus Chikungunya em linhagens de células de mamíferos e mosquitos
title Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
spellingShingle Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
Ribeiro, Ana Cláudia da Silva
Arbovirus / isolamento & purificação
Alphavirus / isolamento & purificação
Vírus Chikungunya / isolamento & purificação
Vírus Mayaro
Linhagem Celular / citologia
title_short Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_full Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_fullStr Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_full_unstemmed Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
title_sort Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages
author Ribeiro, Ana Cláudia da Silva
author_facet Ribeiro, Ana Cláudia da Silva
Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
author_role author
author2 Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro, Ana Cláudia da Silva
Carvalho, Carlos Alberto Marques de
Casseb, Samir Mansour Moraes
Rodrigues, Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
Carvalho, Valéria Lima
dc.subject.decsPrimary.pt_BR.fl_str_mv Arbovirus / isolamento & purificação
Alphavirus / isolamento & purificação
Vírus Chikungunya / isolamento & purificação
Vírus Mayaro
Linhagem Celular / citologia
topic Arbovirus / isolamento & purificação
Alphavirus / isolamento & purificação
Vírus Chikungunya / isolamento & purificação
Vírus Mayaro
Linhagem Celular / citologia
description OBJECTIVE: To trace the infection profile of Mayaro virus (MAYV) and Chikungunya virus (CHIKV) in four different cell lineages. MATERIALS AND METHODS: The isolates of these viruses were used to infect cultures of the following cell lineages – C6/36, VERO, BHK-21, and LLC-MK2 – under the multiplicity of infection of 1 PFU/cell, which were monitored for up to 96 h post-infection (hpi) by phase-contrast microscopy to register the cytopathic effect (CPE); and in addition were subjected to indirect immunofluorescence (iIF) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays for virus antigen detection and RNA load determination, respectively. RESULTS: For both viruses, VERO and BHK-21 cells had CPE of 24 hpi, LLC-MK2 cells presented CPE from 48 hpi, and C6/36 did not present any CPE. In the iIF assays, all cells were already positive at 6 hpi, with the exception of CHIKV-infected LLC-MK2 and BHK-21 cells. Finally, qRT-PCR assays showed that in general cells infected with MAYV presented higher RNA load, although CHIKV-infected LLC-MK2 cells showed the highest RNA load. CONCLUSION: By evaluating the behavior of these arboviruses in different types of cell cultures, including some not commonly used in the laboratory diagnostic routine, the present study provides not only a better comprehension of the replication kinetics of MAYV and CHIKV, but also opportunities for the optimization of their diagnosis in biological samples.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-09-04T17:56:14Z
dc.date.available.fl_str_mv 2019-09-04T17:56:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3883
dc.identifier.issn.-.fl_str_mv 2176-6223
dc.identifier.doi.-.fl_str_mv 10.5123/s2176-62232018000400003
identifier_str_mv RIBEIRO, Ana Cláudia da Silva et al. Infection profiles of Mayaro virus and Chikungunya virus in mammalian and mosquito cell lineages. Revista Pan-Amazônica de Saúde, v. 9, n. 4, p. 25-35, dez. 2018. Disponivel em http://scielo.iec.gov.br/pdf/rpas/v9n4/2176-6223-rpas-9-04-25.pdf. Acesso em 04 set. 2019.
2176-6223
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