Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/3824 |
Resumo: | The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free-born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi-nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi-nested PCR. The presence of infected non-human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species. |
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Silva, Tatiene R. MBarros, Flávia N. LBahia, MicheleSampaio Junior, Francisco DSantos, Sidney S. FInoue, Larissa SGonçalves, Thamirys SChiesorin Neto, LaerzioFaria, Diogo C. L. OTchetto, CamilaViana, Giselle Maria RachidMonteiro, Frederico Ozanan BarrosCavalcante, Gustavo GóesScofield, Alessandra2019-08-09T18:33:28Z2019-08-09T18:33:28Z2019SILVA, Tatiene R. M. et al. Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil. Zoonoses and Public Health, v. xx, n. xx,p. xx, 2019.1863-2378https://patua.iec.gov.br/handle/iec/3824The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free-born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi-nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi-nested PCR. The presence of infected non-human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal Rural University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Sauim Castanheiras Wildlife Refuges. Manaus, AM, Brazil.Chico Mendes Institute for Biodiversity Conservation. Manaus, AM, Brazil.Jungle Warfare Instruction Center, Manaus, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Laboratório de Pesquisa Básica em Malária. Ananindeua, PA, Brasil.Federal Rural University of the Amazon. Institute of Veterinary Medicine. Postgraduate Program in Health and Animal Production in the Amazon. Belém, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.Federal University of Pará. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.engWileyPlasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlasmodium vivax / patogenicidadePlasmodium falciparum / patogenicidadeMalária / veterináriaPrimatas / anatomia & histologiaPrimatas / parasitologiaMonitoramento Epidemiológico / veterináriaReação em Cadeia da Polimerase / métodosAmazônia Brasileira (BR)info:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPlasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil.pdfPlasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/fc74aa75-c81a-4df1-ac6d-3315cbc8909e/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
title |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
spellingShingle |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil Silva, Tatiene R. M Plasmodium vivax / patogenicidade Plasmodium falciparum / patogenicidade Malária / veterinária Primatas / anatomia & histologia Primatas / parasitologia Monitoramento Epidemiológico / veterinária Reação em Cadeia da Polimerase / métodos Amazônia Brasileira (BR) |
title_short |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
title_full |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
title_fullStr |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
title_full_unstemmed |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
title_sort |
Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil |
author |
Silva, Tatiene R. M |
author_facet |
Silva, Tatiene R. M Barros, Flávia N. L Bahia, Michele Sampaio Junior, Francisco D Santos, Sidney S. F Inoue, Larissa S Gonçalves, Thamirys S Chiesorin Neto, Laerzio Faria, Diogo C. L. O Tchetto, Camila Viana, Giselle Maria Rachid Monteiro, Frederico Ozanan Barros Cavalcante, Gustavo Góes Scofield, Alessandra |
author_role |
author |
author2 |
Barros, Flávia N. L Bahia, Michele Sampaio Junior, Francisco D Santos, Sidney S. F Inoue, Larissa S Gonçalves, Thamirys S Chiesorin Neto, Laerzio Faria, Diogo C. L. O Tchetto, Camila Viana, Giselle Maria Rachid Monteiro, Frederico Ozanan Barros Cavalcante, Gustavo Góes Scofield, Alessandra |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Tatiene R. M Barros, Flávia N. L Bahia, Michele Sampaio Junior, Francisco D Santos, Sidney S. F Inoue, Larissa S Gonçalves, Thamirys S Chiesorin Neto, Laerzio Faria, Diogo C. L. O Tchetto, Camila Viana, Giselle Maria Rachid Monteiro, Frederico Ozanan Barros Cavalcante, Gustavo Góes Scofield, Alessandra |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Plasmodium vivax / patogenicidade Plasmodium falciparum / patogenicidade Malária / veterinária Primatas / anatomia & histologia Primatas / parasitologia Monitoramento Epidemiológico / veterinária Reação em Cadeia da Polimerase / métodos Amazônia Brasileira (BR) |
topic |
Plasmodium vivax / patogenicidade Plasmodium falciparum / patogenicidade Malária / veterinária Primatas / anatomia & histologia Primatas / parasitologia Monitoramento Epidemiológico / veterinária Reação em Cadeia da Polimerase / métodos Amazônia Brasileira (BR) |
description |
The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free-born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi-nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi-nested PCR. The presence of infected non-human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-08-09T18:33:28Z |
dc.date.available.fl_str_mv |
2019-08-09T18:33:28Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Tatiene R. M. et al. Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil. Zoonoses and Public Health, v. xx, n. xx,p. xx, 2019. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/3824 |
dc.identifier.issn.-.fl_str_mv |
1863-2378 |
identifier_str_mv |
SILVA, Tatiene R. M. et al. Plasmodium vivax and Plasmodium falciparum infection in Neotropical primates in the western Amazon, Brazil. Zoonoses and Public Health, v. xx, n. xx,p. xx, 2019. 1863-2378 |
url |
https://patua.iec.gov.br/handle/iec/3824 |
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eng |
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eng |
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info:eu-repo/semantics/embargoedAccess |
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Wiley |
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Wiley |
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