Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene

Detalhes bibliográficos
Autor(a) principal: Chapadense, Francesca
Data de Publicação: 2019
Outros Autores: Machado, Ricardo Luiz Dantas, Ventura, Ana Maria Revorêdo da Silva, Áreas, André, Machado, Renato Beilner, Viana, Giselle Maria Rachid, Zalis, Mariano Gustavo, Castro, Ana Maria de, Cravo, Pedro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/3621
Resumo: INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
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spelling Chapadense, FrancescaMachado, Ricardo Luiz DantasVentura, Ana Maria Revorêdo da SilvaÁreas, AndréMachado, Renato BeilnerViana, Giselle Maria RachidZalis, Mariano GustavoCastro, Ana Maria deCravo, Pedro2019-03-29T11:46:27Z2019-03-29T11:46:27Z2019CHAPADENSE, Francesca et al. Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. Revista da Sociedade Brasileira de Medicina Tropical, v. 52, e.20180225, 2019.0037-8682https://patua.iec.gov.br/handle/iec/362110.1590/0037-8682-0225-2018INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.This project received partial federal support from the Evandro Chagas Institute/Brazilian Ministry of Health. This research was also partially financed by FAPESP (São Paulo Research Foundation) through Grant 02/9546-1 and CNPq (National Council for Scientific and Technological Development) through Grant 302353/2003-8Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.Universidade Federal de Fluminense. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Lisboa, Portugal / UniEvangélica. Centro Universitário de Anápolis. Anápolis, GO, Brasil.engSociedade Brasileira de Medicina TropicalPlasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalária / parasitologiaPlasmodium falciparum / patogenicidadeReação em Cadeia da Polimerase / métodosResistência a Medicamentos / efeitos dos fármacosAntimaláricosParasitos / parasitologiainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALPlasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene.pdfPlasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene.pdfapplication/pdf1170779https://patua.iec.gov.br/bitstreams/5e2e82c7-0b4b-4d93-becd-ce8c6e8ff62f/download613cdc9b736a990f7e7c9deb8a643fccMD51LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
title Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
spellingShingle Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
Chapadense, Francesca
Malária / parasitologia
Plasmodium falciparum / patogenicidade
Reação em Cadeia da Polimerase / métodos
Resistência a Medicamentos / efeitos dos fármacos
Antimaláricos
Parasitos / parasitologia
title_short Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
title_full Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
title_fullStr Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
title_full_unstemmed Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
title_sort Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene
author Chapadense, Francesca
author_facet Chapadense, Francesca
Machado, Ricardo Luiz Dantas
Ventura, Ana Maria Revorêdo da Silva
Áreas, André
Machado, Renato Beilner
Viana, Giselle Maria Rachid
Zalis, Mariano Gustavo
Castro, Ana Maria de
Cravo, Pedro
author_role author
author2 Machado, Ricardo Luiz Dantas
Ventura, Ana Maria Revorêdo da Silva
Áreas, André
Machado, Renato Beilner
Viana, Giselle Maria Rachid
Zalis, Mariano Gustavo
Castro, Ana Maria de
Cravo, Pedro
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chapadense, Francesca
Machado, Ricardo Luiz Dantas
Ventura, Ana Maria Revorêdo da Silva
Áreas, André
Machado, Renato Beilner
Viana, Giselle Maria Rachid
Zalis, Mariano Gustavo
Castro, Ana Maria de
Cravo, Pedro
dc.subject.decsPrimary.pt_BR.fl_str_mv Malária / parasitologia
Plasmodium falciparum / patogenicidade
Reação em Cadeia da Polimerase / métodos
Resistência a Medicamentos / efeitos dos fármacos
Antimaláricos
Parasitos / parasitologia
topic Malária / parasitologia
Plasmodium falciparum / patogenicidade
Reação em Cadeia da Polimerase / métodos
Resistência a Medicamentos / efeitos dos fármacos
Antimaláricos
Parasitos / parasitologia
description INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-03-29T11:46:27Z
dc.date.available.fl_str_mv 2019-03-29T11:46:27Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAPADENSE, Francesca et al. Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. Revista da Sociedade Brasileira de Medicina Tropical, v. 52, e.20180225, 2019.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/3621
dc.identifier.issn.-.fl_str_mv 0037-8682
dc.identifier.doi.-.fl_str_mv 10.1590/0037-8682-0225-2018
identifier_str_mv CHAPADENSE, Francesca et al. Plasmodium falciparum malarial parasites from Brazil lack artemisinin resistance-associated mutations in the kelch13 gene. Revista da Sociedade Brasileira de Medicina Tropical, v. 52, e.20180225, 2019.
0037-8682
10.1590/0037-8682-0225-2018
url https://patua.iec.gov.br/handle/iec/3621
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical
dc.source.none.fl_str_mv reponame:Repositório Digital do Instituto Evandro Chagas (Patuá)
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reponame_str Repositório Digital do Instituto Evandro Chagas (Patuá)
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