Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4311 |
Resumo: | Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.) shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication, we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between L. (V.) guyanensis and L. (V.) shawi. |
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Lima, Ana Carolina SGomes, Claudia Maria de CastroTomokane, Thaise YumieCampos, Marliane BatistaZampieri, Ricardo AJorge, Carolina LLaurenti, Márcia DalastraSilveira, Fernando TobiasCorbett, Carlos Eduardo PereiraFloeter-Winter, Lucile Maria2021-05-24T16:54:00Z2021-05-24T16:54:00Z2021LIMA, Ana Carolina S. et al. Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil. Genetics and Molecular Biology, v. 44, n. 2, p. 1-8, 2021.1678-4685https://patua.iec.gov.br/handle/iec/431110.1590/1678-4685-GMB-2020-0123Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.) shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication, we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between L. (V.) guyanensis and L. (V.) shawi.FAPESP (2012/08338-8; 14/50315-0).Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo, SP, Brazil.Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo, SP, Brazil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia. São Paulo, SP, Brazil.Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo, SP, Brazil.engSociedade Brasileira de GenéticaMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniose Cutânea / patologiaReação em Cadeia da Polimerase / métodosEcossistema Amazônicoinfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/7184757c-742e-427d-acd7-2dedfa6c77fe/download11832eea31b16df8613079d742d61793MD52ORIGINALMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil.pdfMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil.pdfapplication/pdf1099665https://patua.iec.gov.br/bitstreams/23716179-c104-4614-8ae6-19ee4f56e6e6/downloadf92771f675682cdf51932d2c5ead94b3MD51TEXTMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil.pdf.txtMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil.pdf.txtExtracted texttext/plain40613https://patua.iec.gov.br/bitstreams/34cfd9f8-74fa-4c17-9826-0e1f029ae384/downloadb2672b4d02909fbdb9e3a32460ab648dMD55THUMBNAILMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil.pdf.jpgMolecular tools confirm natural Leishmania (Viannia) guyanensis/L. 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dc.title.pt_BR.fl_str_mv |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
title |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
spellingShingle |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil Lima, Ana Carolina S Leishmaniose Cutânea / patologia Reação em Cadeia da Polimerase / métodos Ecossistema Amazônico |
title_short |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
title_full |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
title_fullStr |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
title_full_unstemmed |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
title_sort |
Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil |
author |
Lima, Ana Carolina S |
author_facet |
Lima, Ana Carolina S Gomes, Claudia Maria de Castro Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Márcia Dalastra Silveira, Fernando Tobias Corbett, Carlos Eduardo Pereira Floeter-Winter, Lucile Maria |
author_role |
author |
author2 |
Gomes, Claudia Maria de Castro Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Márcia Dalastra Silveira, Fernando Tobias Corbett, Carlos Eduardo Pereira Floeter-Winter, Lucile Maria |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Ana Carolina S Gomes, Claudia Maria de Castro Tomokane, Thaise Yumie Campos, Marliane Batista Zampieri, Ricardo A Jorge, Carolina L Laurenti, Márcia Dalastra Silveira, Fernando Tobias Corbett, Carlos Eduardo Pereira Floeter-Winter, Lucile Maria |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Leishmaniose Cutânea / patologia Reação em Cadeia da Polimerase / métodos Ecossistema Amazônico |
topic |
Leishmaniose Cutânea / patologia Reação em Cadeia da Polimerase / métodos Ecossistema Amazônico |
description |
Seven isolates from patients with American cutaneous leishmaniasis in the Amazon region of Brazil were phenotypically suggestive of Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids. In this work, two molecular targets were employed to check the hybrid identity of the putative hybrids. Heat shock protein 70 (hsp70) gene sequences were analyzed by three different polymerase chain reaction (PCR) approaches, and two different patterns of inherited hsp70 alleles were found. Three isolates presented heterozygous L. (V.) guyanensis/L. (V.) shawi patterns, and four presented homozygous hsp70 patterns involving only L. (V.) shawi alleles. The amplicon sequences confirmed the RFLP patterns. The high-resolution melting method detected variant heterozygous and homozygous profiles. Single-nucleotide polymorphism genotyping/cleaved amplified polymorphic site analysis suggested a higher contribution from L. (V.) guyanensis in hsp70 heterozygous hybrids. Additionally, PCR-RFLP analysis targeting the enzyme mannose phosphate isomerase (mpi) gene indicated heterozygous and homozygous cleavage patterns for L. (V.) shawi and L. (V.) guyanensis, corroborating the hsp70 findings. In this communication, we present molecular findings based on partial informative regions of the coding sequences of hsp70 and mpi as markers confirming that some of the parasite strains from the Brazilian Amazon region are indeed hybrids between L. (V.) guyanensis and L. (V.) shawi. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-05-24T16:54:00Z |
dc.date.available.fl_str_mv |
2021-05-24T16:54:00Z |
dc.date.issued.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LIMA, Ana Carolina S. et al. Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil. Genetics and Molecular Biology, v. 44, n. 2, p. 1-8, 2021. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4311 |
dc.identifier.issn.-.fl_str_mv |
1678-4685 |
dc.identifier.doi.-.fl_str_mv |
10.1590/1678-4685-GMB-2020-0123 |
identifier_str_mv |
LIMA, Ana Carolina S. et al. Molecular tools confirm natural Leishmania (Viannia) guyanensis/L. (V.) shawi hybrids causing cutaneous leishmaniasis in the Amazon region of Brazil. Genetics and Molecular Biology, v. 44, n. 2, p. 1-8, 2021. 1678-4685 10.1590/1678-4685-GMB-2020-0123 |
url |
https://patua.iec.gov.br/handle/iec/4311 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Sociedade Brasileira de Genética |
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Sociedade Brasileira de Genética |
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