Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4712 |
Resumo: | The genus Ctenomys has been widely used in karyotype evolution studies due to the variation in their diploid numbers. Ctenomys minutus is characterized by intraspecific variation in diploid number (2n = 42, 46, 48, and 50), which makes it an interesting model to investigate genomic rearrangements mechanisms that could lead to different cytotypes in this species. Thereupon, it has been already shown that DNA methylation may participate in chromosome structure. Therefore, we aimed to investigate whether telomeres and global DNA methylation had a role in the genome rearrangements that led to this variation in C. minutus. We also realized an analysis for the presence of intrachromosomal telomeric repeats (ITRs) by fluorescence in situ hybridization. Our study demonstrated that neither telomere length nor DNA methylation had significant differences among the cytotypes. However, if only females were considered, there were significant differences for telomere length and methylation. Young individuals, regardless of their cytotypes, had the most methylated DNA. Regarding the ITRs, we found a signal on chromosome 1 in 2n = 50b. No evidence was found that telomere length or methylation could have influenced chromosomal rearrangements, although new cytotypes seem to have emerged within the distribution of parental cytotypes by the accumulation of different chromosomal rearrangements. |
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Matzenbacher, Cristina ASilva, Juliana daGarcia, Ana Leticia HKretschmer, RafaelCappetta, MónicaOliveira, Edivaldo Herculano Corrêa deFreitas, Thales R. O. de2022-11-08T12:23:01Z2022-11-08T12:23:01Z2022MATZENBACHER, Cristina A. et al. Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal). Genome, v. 65, n. 11, p. 563-572, Nov. 2022. DOI: https://doi.org/10.1139/gen-2022-0018.1480-3321https://patua.iec.gov.br/handle/iec/471210.1139/gen-2022-0018The genus Ctenomys has been widely used in karyotype evolution studies due to the variation in their diploid numbers. Ctenomys minutus is characterized by intraspecific variation in diploid number (2n = 42, 46, 48, and 50), which makes it an interesting model to investigate genomic rearrangements mechanisms that could lead to different cytotypes in this species. Thereupon, it has been already shown that DNA methylation may participate in chromosome structure. Therefore, we aimed to investigate whether telomeres and global DNA methylation had a role in the genome rearrangements that led to this variation in C. minutus. We also realized an analysis for the presence of intrachromosomal telomeric repeats (ITRs) by fluorescence in situ hybridization. Our study demonstrated that neither telomere length nor DNA methylation had significant differences among the cytotypes. However, if only females were considered, there were significant differences for telomere length and methylation. Young individuals, regardless of their cytotypes, had the most methylated DNA. Regarding the ITRs, we found a signal on chromosome 1 in 2n = 50b. No evidence was found that telomere length or methylation could have influenced chromosomal rearrangements, although new cytotypes seem to have emerged within the distribution of parental cytotypes by the accumulation of different chromosomal rearrangements.Universidade Federal do Rio Grande do Sul (UFRGS), Universidade Luterana do Brasil (ULBRA), Universidad de la República (UDELAR), and Brazilian agencies: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—finance code 001, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Proc. PDJ 153212/2018-7), and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS).Universidade Federal do Rio Grande do Sul. Departamento de Genética. Laboratório de Citogenética e Evolução. Porto Alegre, RS, Brazil.Universidade Luterana do Brasil. Laboratório de Genética Toxicológica. Canoas, RS, Brazil.Universidade Luterana do Brasil. Laboratório de Genética Toxicológica. Canoas, RS, Brazil.Universidade Federal de Pelotas. Departamento de Ecologia, Zoologia e Genética. Pelotas, RS, Brazil.Universidad de la República. Facultad de Medicina. Departamento de Genética. Laboratorio de Epidemiología Genética. Montevideo, Uruguay.Universidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Faculdade de Ciências Naturais. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Laboratório de Citogenética e Cultura de Tecidos. Ananindeua, PA, Brasil.Universidade Federal do Rio Grande do Sul. Departamento de Genética. Laboratório de Citogenética e Evolução. Porto Alegre, RS, Brazil.engCanadian Science PublishingUsing telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCariotipagem / métodosCariotipagem / veterináriaAnálise CitogenéticaTelômero / genéticaRoedor Fossorialinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALAcesso Embargado.pdfAcesso Embargado.pdfapplication/pdf551083https://patua.iec.gov.br/bitstreams/8d3b7bd6-c855-43e6-89f5-41ffb9d5f4f1/downloadc9a9c128e29cac82a5d7fdf3f4e6da73MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/fbdf79c9-ed3e-4503-8aae-860506024225/download11832eea31b16df8613079d742d61793MD52TEXTAcesso Embargado.pdf.txtAcesso Embargado.pdf.txtExtracted texttext/plain2https://patua.iec.gov.br/bitstreams/5f20b640-7e7e-4648-9fef-f7c39b59930c/downloade1c06d85ae7b8b032bef47e42e4c08f9MD53THUMBNAILAcesso Embargado.pdf.jpgAcesso Embargado.pdf.jpgGenerated Thumbnailimage/jpeg3095https://patua.iec.gov.br/bitstreams/1bbac846-47d2-46c8-a49c-ae1580a25d44/download71859d578212107f7f8c49a4ce09d9eeMD54iec/47122022-12-21 14:23:39.817oai:patua.iec.gov.br:iec/4712https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-12-21T14:23:39Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
title |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
spellingShingle |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) Matzenbacher, Cristina A Cariotipagem / métodos Cariotipagem / veterinária Análise Citogenética Telômero / genética Roedor Fossorial |
title_short |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
title_full |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
title_fullStr |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
title_full_unstemmed |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
title_sort |
Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal) |
author |
Matzenbacher, Cristina A |
author_facet |
Matzenbacher, Cristina A Silva, Juliana da Garcia, Ana Leticia H Kretschmer, Rafael Cappetta, Mónica Oliveira, Edivaldo Herculano Corrêa de Freitas, Thales R. O. de |
author_role |
author |
author2 |
Silva, Juliana da Garcia, Ana Leticia H Kretschmer, Rafael Cappetta, Mónica Oliveira, Edivaldo Herculano Corrêa de Freitas, Thales R. O. de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Matzenbacher, Cristina A Silva, Juliana da Garcia, Ana Leticia H Kretschmer, Rafael Cappetta, Mónica Oliveira, Edivaldo Herculano Corrêa de Freitas, Thales R. O. de |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Cariotipagem / métodos Cariotipagem / veterinária Análise Citogenética Telômero / genética Roedor Fossorial |
topic |
Cariotipagem / métodos Cariotipagem / veterinária Análise Citogenética Telômero / genética Roedor Fossorial |
description |
The genus Ctenomys has been widely used in karyotype evolution studies due to the variation in their diploid numbers. Ctenomys minutus is characterized by intraspecific variation in diploid number (2n = 42, 46, 48, and 50), which makes it an interesting model to investigate genomic rearrangements mechanisms that could lead to different cytotypes in this species. Thereupon, it has been already shown that DNA methylation may participate in chromosome structure. Therefore, we aimed to investigate whether telomeres and global DNA methylation had a role in the genome rearrangements that led to this variation in C. minutus. We also realized an analysis for the presence of intrachromosomal telomeric repeats (ITRs) by fluorescence in situ hybridization. Our study demonstrated that neither telomere length nor DNA methylation had significant differences among the cytotypes. However, if only females were considered, there were significant differences for telomere length and methylation. Young individuals, regardless of their cytotypes, had the most methylated DNA. Regarding the ITRs, we found a signal on chromosome 1 in 2n = 50b. No evidence was found that telomere length or methylation could have influenced chromosomal rearrangements, although new cytotypes seem to have emerged within the distribution of parental cytotypes by the accumulation of different chromosomal rearrangements. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-11-08T12:23:01Z |
dc.date.available.fl_str_mv |
2022-11-08T12:23:01Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MATZENBACHER, Cristina A. et al. Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal). Genome, v. 65, n. 11, p. 563-572, Nov. 2022. DOI: https://doi.org/10.1139/gen-2022-0018. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4712 |
dc.identifier.issn.-.fl_str_mv |
1480-3321 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1139/gen-2022-0018 |
identifier_str_mv |
MATZENBACHER, Cristina A. et al. Using telomeric length measurements and methylation to understand the karyotype diversification of Ctenomys minutus (a small fossorial mammal). Genome, v. 65, n. 11, p. 563-572, Nov. 2022. DOI: https://doi.org/10.1139/gen-2022-0018. 1480-3321 10.1139/gen-2022-0018 |
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https://patua.iec.gov.br/handle/iec/4712 |
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eng |
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eng |
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Canadian Science Publishing |
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Canadian Science Publishing |
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