Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4544 |
Resumo: | Two species of manatees are found in Northern Brazil—the Antillean manatee (Trichechus manatus), which is found along the coast from Florida to Northeastern Brazil, and the Amazonian manatee (Trichechus inunguis), endemic to the Amazon drainage basin. These species show a sympatric distribution in the region of the Marajó Archipelago, an estuarine area surrounding the Amazon River mouth. There is evidence of the occurrence of interspecific hybrids in this area, based on mitochondrial DNA analyses, although the use of nuclear markers has not corroborated this proposal. Considering that these species show very distinct karyotypes, despite being closely related (2n = 48 in T. manatus and 2n = 56 in T. inunguis), hybrids would present distinct chromosome numbers. Based on this, we conducted cytogenetic analyses using classic and molecular techniques in three calves found stranded in the Marajó Island and Amapá coast. The results showed that one of them, morphologically classified as T. inunguis, presented the correspondent karyotype, with 2n = 56. However, the other two, which were phenotypically similar to T. manatus, showed 2n = 49. Despite the same diploid number, their G-banding patterns revealed some differences. The results of the distribution of some microsatellite sequences have also confirmed the heterozygosity of some chromosomal pairs in these two individuals. These results are the first indubitable confirmation of the occurrence of natural hybrids between T. manatus and T. inunguis, and also brings about some issues concerning the viability of hybrids, considering that these two individuals do not correspond to an F1 hybrid, but instead, both presented a possible F2 karyotype. |
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Oliveira, Edivaldo Herculano Corrêa deGomes, Anderson J. BCosta, Alexandra FLima, Renata EminBonvicino, Cibele RViana, Maria CReis, Laura M. AVidal, Marcelo DCavalcanti, Mirella V, GAttademo, Fernanda L. NLuna, Fábio OSiciliano, Salvatore2022-06-03T12:39:25Z2022-06-03T12:39:25Z2022OLIVEIRA, Edivaldo Herculano Corrêa de et al. Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis. Life, v. 12, n. 5, p. 1-12, Apr. 2022.2075-1729https://patua.iec.gov.br/handle/iec/454410.3390/life12050616Two species of manatees are found in Northern Brazil—the Antillean manatee (Trichechus manatus), which is found along the coast from Florida to Northeastern Brazil, and the Amazonian manatee (Trichechus inunguis), endemic to the Amazon drainage basin. These species show a sympatric distribution in the region of the Marajó Archipelago, an estuarine area surrounding the Amazon River mouth. There is evidence of the occurrence of interspecific hybrids in this area, based on mitochondrial DNA analyses, although the use of nuclear markers has not corroborated this proposal. Considering that these species show very distinct karyotypes, despite being closely related (2n = 48 in T. manatus and 2n = 56 in T. inunguis), hybrids would present distinct chromosome numbers. Based on this, we conducted cytogenetic analyses using classic and molecular techniques in three calves found stranded in the Marajó Island and Amapá coast. The results showed that one of them, morphologically classified as T. inunguis, presented the correspondent karyotype, with 2n = 56. However, the other two, which were phenotypically similar to T. manatus, showed 2n = 49. Despite the same diploid number, their G-banding patterns revealed some differences. The results of the distribution of some microsatellite sequences have also confirmed the heterozygosity of some chromosomal pairs in these two individuals. These results are the first indubitable confirmation of the occurrence of natural hybrids between T. manatus and T. inunguis, and also brings about some issues concerning the viability of hybrids, considering that these two individuals do not correspond to an F1 hybrid, but instead, both presented a possible F2 karyotype.This research was funded by Projeto Piatam Mar and Piatam Oceano/Petrobras, Projeto Bicho D’água/Petrobras, Projeto Inova-Fiocruz, Vale/Fundação de Amparo à Pesquisa do Estado de São Paulo/Fundação de Amparo à Pesquisa do Estado do Pará/Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPESP/FAPESPA/FAPEMIG), grant number 038/2011, PAPQ-PROPESP/UFPA (Edital 02/2022). C.R.B. was funded by CNPq and FAPERJ (304498/2014-9, E26/201.200/2014), S.S. by CNPq (306076/2019-5), R.E.-L. by CNPqUniversidade Federal do Pará. Instituto de Ciências Exatas e Naturais. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilInstituto Federal do Pará. Abaetetuba, PA, BrazilMuseu Paraense Emílio Goeldi. Belém, PA, Brazil / Instituto Bicho D’água. Belém, PA, BrazilMuseu Paraense Emílio Goeldi. Belém, PA, Brazil / Instituto Bicho D’água. Belém, PA, BrazilInstituto Nacional do Câncer. Rio de Janeiro, RJ, BrazilInstituto Nacional do Câncer. Rio de Janeiro, RJ, Brazil / Universidade Estadual de Campinas. Instituto de Biologia. Departamento de Biologia Animal. Campinas, SP, BrazilNúcleo de Gestão Integrada. São Luís, MA, BrazilCentro Nacional de Pesquisa e Conservação da Sociobiodiversidade Associada a Povos e Comunidades Tradicionais. São Luís, MA, BrazilInstituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis. Macapá, AP, BrazilInstituto Chico Mendes de Conservação de Biodiversidade. Centro Nacional de Pesquisa e Conservação de Mamíferos Aquáticos. Santos, SP, BrazilInstituto Chico Mendes de Conservação de Biodiversidade. Centro Nacional de Pesquisa e Conservação de Mamíferos Aquáticos. Santos, SP, BrazilFundação Oswaldo Cruz, Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, BrazilengMDPIKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePeixes / anatomia & histologiaTrichechus manatus / genéticaTrichechus inunguis / genéticaCariotipagem / métodosIntrogressão Genética / genéticaHibridização GenéticaArquipélago do Marajó (PA)info:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECLICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/f26d62c8-1196-48da-b66f-8074ceaf8c8f/download11832eea31b16df8613079d742d61793MD52ORIGINALKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdfKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdfapplication/pdf3396068https://patua.iec.gov.br/bitstreams/ac5106e4-051d-4211-993b-5cdd363e5ff8/download16650dd19557f4adc3db352565181962MD51TEXTKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdf.txtKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdf.txtExtracted texttext/plain44148https://patua.iec.gov.br/bitstreams/9c8faba3-5f27-43f8-91f1-f9245b203326/download4ada86ea7fe24aca9ddcad1cc9e27092MD55THUMBNAILKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdf.jpgKaryotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis.pdf.jpgGenerated Thumbnailimage/jpeg5615https://patua.iec.gov.br/bitstreams/0dbd79ba-b5a4-47ca-affe-2fbf9cdc7b48/downloade42ac2fc3f5f83528ee27280f0aee8cbMD56iec/45442022-10-20 22:06:44.617oai:patua.iec.gov.br:iec/4544https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T22:06:44Repositório Digital do Instituto Evandro Chagas (Patuá) - 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dc.title.pt_BR.fl_str_mv |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
title |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
spellingShingle |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis Oliveira, Edivaldo Herculano Corrêa de Peixes / anatomia & histologia Trichechus manatus / genética Trichechus inunguis / genética Cariotipagem / métodos Introgressão Genética / genética Hibridização Genética Arquipélago do Marajó (PA) |
title_short |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
title_full |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
title_fullStr |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
title_full_unstemmed |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
title_sort |
Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis |
author |
Oliveira, Edivaldo Herculano Corrêa de |
author_facet |
Oliveira, Edivaldo Herculano Corrêa de Gomes, Anderson J. B Costa, Alexandra F Lima, Renata Emin Bonvicino, Cibele R Viana, Maria C Reis, Laura M. A Vidal, Marcelo D Cavalcanti, Mirella V, G Attademo, Fernanda L. N Luna, Fábio O Siciliano, Salvatore |
author_role |
author |
author2 |
Gomes, Anderson J. B Costa, Alexandra F Lima, Renata Emin Bonvicino, Cibele R Viana, Maria C Reis, Laura M. A Vidal, Marcelo D Cavalcanti, Mirella V, G Attademo, Fernanda L. N Luna, Fábio O Siciliano, Salvatore |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Edivaldo Herculano Corrêa de Gomes, Anderson J. B Costa, Alexandra F Lima, Renata Emin Bonvicino, Cibele R Viana, Maria C Reis, Laura M. A Vidal, Marcelo D Cavalcanti, Mirella V, G Attademo, Fernanda L. N Luna, Fábio O Siciliano, Salvatore |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Peixes / anatomia & histologia Trichechus manatus / genética Trichechus inunguis / genética Cariotipagem / métodos Introgressão Genética / genética Hibridização Genética Arquipélago do Marajó (PA) |
topic |
Peixes / anatomia & histologia Trichechus manatus / genética Trichechus inunguis / genética Cariotipagem / métodos Introgressão Genética / genética Hibridização Genética Arquipélago do Marajó (PA) |
description |
Two species of manatees are found in Northern Brazil—the Antillean manatee (Trichechus manatus), which is found along the coast from Florida to Northeastern Brazil, and the Amazonian manatee (Trichechus inunguis), endemic to the Amazon drainage basin. These species show a sympatric distribution in the region of the Marajó Archipelago, an estuarine area surrounding the Amazon River mouth. There is evidence of the occurrence of interspecific hybrids in this area, based on mitochondrial DNA analyses, although the use of nuclear markers has not corroborated this proposal. Considering that these species show very distinct karyotypes, despite being closely related (2n = 48 in T. manatus and 2n = 56 in T. inunguis), hybrids would present distinct chromosome numbers. Based on this, we conducted cytogenetic analyses using classic and molecular techniques in three calves found stranded in the Marajó Island and Amapá coast. The results showed that one of them, morphologically classified as T. inunguis, presented the correspondent karyotype, with 2n = 56. However, the other two, which were phenotypically similar to T. manatus, showed 2n = 49. Despite the same diploid number, their G-banding patterns revealed some differences. The results of the distribution of some microsatellite sequences have also confirmed the heterozygosity of some chromosomal pairs in these two individuals. These results are the first indubitable confirmation of the occurrence of natural hybrids between T. manatus and T. inunguis, and also brings about some issues concerning the viability of hybrids, considering that these two individuals do not correspond to an F1 hybrid, but instead, both presented a possible F2 karyotype. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-03T12:39:25Z |
dc.date.available.fl_str_mv |
2022-06-03T12:39:25Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Edivaldo Herculano Corrêa de et al. Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis. Life, v. 12, n. 5, p. 1-12, Apr. 2022. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4544 |
dc.identifier.issn.-.fl_str_mv |
2075-1729 |
dc.identifier.doi.-.fl_str_mv |
10.3390/life12050616 |
identifier_str_mv |
OLIVEIRA, Edivaldo Herculano Corrêa de et al. Karyotypical confirmation of natural hybridization between two manatee species, Trichechus manatus and Trichechus inunguis. Life, v. 12, n. 5, p. 1-12, Apr. 2022. 2075-1729 10.3390/life12050616 |
url |
https://patua.iec.gov.br/handle/iec/4544 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
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